Long noncoding RNA LSINCT5 promotes endometrial carcinoma cell proliferation, cycle, and invasion by promoting the Wnt/β-catenin signaling pathway via HMGA2

Abstract

Background: A review of the evidence has indicated the critical role of long noncoding RNA (lncRNA) LSINCT5 in a large number of human cancers. However, the mechanistic involvement of LSINCT5 in endometrial carcinoma (EC) is still unknown. Here the authors aim to characterize the expression status of LSINCT5 and elucidate its mechanistic relevance to EC. Methods: Relative expression of LSINCT5 and HMGA2 were quantified by a real-time polymerase chain reaction. SiRNAs were employed to specifically knockdown endogenous LSINCT5 in EC cells. Cell proliferation was measured with Cell Count Kit-8 kit (CCK-8, Dojindo, Kumamoto, Japan) and cell growth was assessed by a colony formation assay. The cell cycle was analyzed with propidium iodide (PI) staining. Apoptotic cells were determined by flow cytometry after Annexin V/PI double-staining. Cell migration was evaluated by a wound-healing assay, and cell invasion was assessed using a transwell migration assay. The protein levels of HMGA2, Wnt3a, p-β-catenin, c-myc, β-actin, and GAPDH were determined by western blot. Results: The authors observed positively correlated and aberrantly up-regulated LSINCT5 and HMGA2 in EC. LSINCT5 deficiency significantly inhibited cell proliferation, cell cycle progression, and induced apoptosis. Meanwhile, cell migration and invasion were greatly compromised by the LSINCT5 knockdown. LSINCT5 stabilized HMGA2, which subsequently stimulated activation of Wnt/β-catenin signaling and consequently contributed to the oncogenic properties of LSINCT5 in EC. Conclusions: Our data uncovered the oncogenic activities and highlighted the mechanistic contributions of the LSINCT5-HMGA2-Wnt/β-catenin signaling pathway in EC.

Identification of long noncoding RNA RP11-89K21.1 and RP11-357H14.17 as prognostic signature of endometrial carcinoma via integrated bioinformatics analysis

“…We further explored the potential role of ceRNA network mechanism regulated by RP11-89K21. promoted proliferation, invasion and metastasis of EC cells by regulating HMGA2/Wnt/β-catenin signaling pathway [45]. LncRNA OGFRP1 promoted the malignant progression of endometrial carcinoma by regulating the miR124-3p/SIRT1 axis and activating the PI3K/AKT/GSK-3β pathway [46].…”

Identification of long noncoding RNA RP11-89K21.1 and RP11-357H14.17 as prognostic signature of endometrial carcinoma via integrated bioinformatics analysis

“…Many studies have shown that lncRNAs affects the initiation and development of tumors by regulating a variety of molecular mechanisms and signaling pathways. Researchers observed lncRNA LSINCT5 promoted proliferation, invasion and metastasis of EC cells by regulating HMGA2/Wnt/β-catenin signaling pathway [45]. LncRNA OGFRP1 promoted the malignant progression of endometrial carcinoma by regulating the miR124-3p/SIRT1 axis and activating the PI3K/AKT/GSK-3β pathway [46].…”

Knockdown of ectodysplasin‑A receptor‑associated adaptor protein exerts a tumor‑suppressive effect in tongue squamous cell carcinoma cells

“…In addition, a study indicated that the expression of MYC target genes was inhibited in response to the downregulation of Gcn5 histone acetyltransferase activity, contributing to apoptosis of lymphoma cells (60). c-MYC was also found to be repressed in response to long stress-induced non-coding transcript 5 knockdown, which inhibited tumor cell proliferation, thereby stimulating apoptosis of cells (61).…”