Long noncoding RNA NEAT1 promotes cell proliferation, migration, and invasion in hepatocellular carcinoma through interacting with miR‐384

Zhū, Lìyǐng; Yang, Ning; Li, Chengcheng; Liu, Guoqi; Pan, Wei; Li, Xing

doi:10.1002/jcb.27499

Cited by 22 publications

(16 citation statements)

References 25 publications

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“…affect the sensitivity to sorafenib and radiotherapy, as well as immune escape in HCC in vitro. Consistent with this role, aberrant expression of lncRNA-NEAT1 has been demonstrated in HCC and is associated with poor survival of HCC patients (11,(19)(20)(21). Liu et al demonstrated that high expression of lncRNA-NEAT1 in a Chinese population is an independent risk factor for poor survival of patients with HCC (22).…”

Section: Figure 5 |mentioning

confidence: 81%

“…The lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) has been reported as a novel player in the onset and progression of HCC. Overexpression of NEAT1 drives HCC progression and has been correlated with the poor prognosis of patients (11,12). Furthermore, Choudhry et al demonstrated that lncRNA-NEAT1 activation in response to hypoxia promotes the survival of breast cancer cells (13).…”

Section: Introductionmentioning

confidence: 99%

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Hypoxia-Induced lncRNA-NEAT1 Sustains the Growth of Hepatocellular Carcinoma via Regulation of miR-199a-3p/UCK2

et al. 2020

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The long noncoding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) has emerged as a novel player in hepatocellular carcinoma (HCC). Hypoxia is a common characteristic of the microenvironment of HCC. This study aimed to investigate whether lncRNA-NEAT1 is induced by hypoxia in HCC, and the mechanism that underlies LncRNA-NEAT1 function. Methods: The expression changes of lncRNA-NEAT1 in HCC cell lines under hypoxic conditions were examined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The regulatory effect of HIF-1α on lncRNA-NEAT1 was confirmed with chromatin immunoprecipitation (ChIP) and luciferase reporter assays. The function of lncRNA-NEAT1 on HCC cell growth under hypoxic conditions was determined by CCK-8 assay and flow cytometry. lncRNA-NEAT1 was predicted to serve as a competing endogenous RNA (ceRNA) within microRNA (miRNA)/mRNA axes based on microarray data, public HCC-related datasets and integrative bioinformatics analysis, and the miR-199a-3p/UCK2 axis was selected and validated by qRT-PCR, western blotting, RNA immunoprecipitation, and luciferase reporter analyses. The role of miR-199a-3p/UCK2 in HCC and its functional association with lncRNA-NEAT1 were assessed both in vitro and in vivo. Results: LncRNA-NEAT1 expression was significantly induced by hypoxia in SNU-182 and HUH7 cells. HIF-1α was shown to regulate lncRNA-NEAT1 transcription. Under hypoxic conditions, lncRNA-NEAT1 maintained the growth of HCC cells and inhibited apoptosis and cell cycle arrest. LncRNA-NEAT1 was predicted to regulate a panel of HCC-associated miRNA-mRNA pairs consisting of 8 miRNAs and 13 mRNAs. LncRNA-NEAT1 was shown to function as a ceRNA of miR-199a-3p/UCK2 both in HCC cells under hypoxic conditions and in an animal model. Conclusion: LncRNA-NEAT1 is a hypoxia-responsive lncRNA in HCC cell lines Insilico evidence suggested that LncRNA-NEAT1 may sustainthe growth of HCC cells by Zhang et al. LncRNA-NEAT1 in HCC Under Hypoxia regulating HCC-associated mRNAs that interact with tumor-suppressive miRNAs. The lncRNA-NEAT1/miR-199a-3p/UCK2 pathway may contribute to the progression of HCC cell lines in a hypoxic microenvironment and therefore may represent a novel therapeutic target for HCC.

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Section: Figure 5 |mentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Hypoxia-Induced lncRNA-NEAT1 Sustains the Growth of Hepatocellular Carcinoma via Regulation of miR-199a-3p/UCK2

et al. 2020

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“…Dysregulated lncRNAs have been identified to be related to the disease development (Yu, Chuang, & Kuo, ). NEAT1 has been confirmed to participate in cell proliferation (Wang, Wang, Zhang, Deng, & Long, ; Zhu et al., ), so we hypothesized that NEAT1 may be involved in the pathogenesis of osteosarcoma.…”

Section: Discussionmentioning

confidence: 93%

Long noncoding RNA NEAT1 regulates the development of osteosarcoma through sponging miR‐34a‐5p to mediate HOXA13 expression as a competitive endogenous RNA

Wang

Zhao

et al. 2019

Molec Gen & Gen Med

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Background Long noncoding RNA (lncRNA) exerts a potential regulatory role in tumorigenesis. LncRNA NEAT1 expression remains high in osteosarcoma tissues. However, its biological mechanism in osteosarcoma remains unknown. Methods In this study, NEAT1 expression in osteosarcoma cells was detected by qRT‐PCR. Proliferative and apoptosis potentials of osteosarcoma cells were determined by CCK‐8 assay and Flow Cytometry, respectively. We identified the potential target of NEAT1 through bioinformatics and dual‐luciferase reporter gene assay. Furthermore, their interaction and functions in regulating the development of osteosarcoma were clarified by Western blot and RIP assay. Results Our results demonstrated a high expression of NEAT1 in osteosarcoma tissues and cells. Overexpression of NEAT1 markedly accelerated proliferative and reduced apoptosis potentials of osteosarcoma cells. Besides, NEAT1 could positively regulate the expression of HOXA13 by competing with miR‐34a‐5p. Conclusion These results indicated that NEAT1 participated in the development of osteosarcoma as a ceRNA to competitively bind to miR‐34a‐5p and thus mediate HOXA13 expression.

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“…Furthermore, lncRNA-NEAT1 has been reported to affect the sensitivity to sorafenib and radiotherapy, as well as immune escape in HCC in vitro. Consistent with this role, aberrant expression of lncRNA-NEAT1 has been demonstrated in HCC and is associated with poor survival of HCC patients [11,[16][17][18]. Liu et al demonstrated that high expression of lncRNA-NEAT1 in a Chinese population is an independent risk factor for poor survival of patients with HCC [19].…”

Section: Lncrna-neat1 Promotes Hcc Tumor Growth Through Mir-199a-3p/umentioning

confidence: 81%

Hypoxia-induced lncRNA -NEAT1 sustains the growth of hepatocellular carcinoma via regulation of miR-199a-3p/UCK2: integrative bioinformatics analysis and experimental validation

Zhang

Qian²,

Xia

et al. 2020

Preprint

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Background: The long noncoding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) has emerged as a novel player in hepatocellular carcinoma (HCC). Hypoxia is a common characteristic of the microenvironment of HCC. However, it remains unclear whether lncRNA-NEAT1 is induced by hypoxia in HCC, and the mechanism that underlies LncRNA-NEAT1 function is not well characterized. Methods: The expression changes of lncRNA-NEAT1 in HCC cell lines under hypoxic conditions were examined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The regulatory effect of HIF-1α on lncRNA-NEAT1 was confirmed with chromatin immunoprecipitation (ChIP) and luciferase reporter assays. The function of lncRNA-NEAT1 on HCC cell growth under hypoxic conditions was determined by CCK-8 assay and flow cytometry. lncRNA -NEAT1 was predicted to serve as a competing endogenous RNA (ceRNA) within microRNA (miRNA)/mRNA axes based on microarray data, public HCC-related datasets and integrative bioinformatics analysis, and the miR-199a-3p/UCK2 axis was selected and validated by qRT-PCR, western blotting, RNA immunoprecipitation, and luciferase reporter analyses. The role of miR-199a-3p/UCK2 in HCC and its functional association with lncRNA-NEAT1 were assessed both in vitro and in vivo . Results : LncRNA-NEAT1 expression was significantly induced by hypoxia in SNU-182 and HUH7 cells. HIF-1α was shown to regulate lncRNA-NEAT1 transcription. Under hypoxic conditions, lncRNA-NEAT1 maintained the growth of HCC cells and inhibited apoptosis and cell cycle arrest. LncRNA-NEAT1 was predicted to regulate a panel of HCC-associated miRNA-mRNA pairs consisting of 8 miRNAs and 13 mRNAs. Furthermore, lncRNA-NEAT1 was demonstrated to serve as a miR-199a-3p sponge that regulates UCK2 expression and to play a tumor-suppressive role in HCC, while UCK2 promotes cell growth. LncRNA-NEAT1 was shown to function as a ceRNA of miR-199a-3p/UCK2 both in HCC cells under hypoxic conditions and in an animal model. Conclusion: LncRNA-NEAT1 is a hypoxia-responsive lncRNA in HCC that sustains the growth of HCC cells by regulating HCC-associated mRNAs that interact with tumor-suppressive miRNAs. The lncRNA-NEAT1/miR-199a-3p/UCK2 pathway may contribute to the progression of HCC in a hypoxic microenvironment and therefore may represent a novel therapeutic target for HCC.

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Long noncoding RNA NEAT1 promotes cell proliferation, migration, and invasion in hepatocellular carcinoma through interacting with miR‐384

Cited by 22 publications

References 25 publications

Hypoxia-Induced lncRNA-NEAT1 Sustains the Growth of Hepatocellular Carcinoma via Regulation of miR-199a-3p/UCK2

Hypoxia-Induced lncRNA-NEAT1 Sustains the Growth of Hepatocellular Carcinoma via Regulation of miR-199a-3p/UCK2

Long noncoding RNA NEAT1 regulates the development of osteosarcoma through sponging miR‐34a‐5p to mediate HOXA13 expression as a competitive endogenous RNA

Hypoxia-induced lncRNA -NEAT1 sustains the growth of hepatocellular carcinoma via regulation of miR-199a-3p/UCK2: integrative bioinformatics analysis and experimental validation

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