Long noncoding RNA NEAT1 promotes nasopharyngeal carcinoma progression through regulation of miR-124/NF-κB pathway

Abstract

Nasopharyngeal carcinoma (NPC) is one of the most common malignancies and seriously endangers people’s health. Recently, long noncoding RNA (lncRNA) NEAT1 has been determined as an oncogenic gene in a variety of cancers. However, the effect of NEAT1 in NPC and its underlying mechanism have not been well elaborated. In this study, the data showed that NEAT1 was upregulated and miR-124 was downregulated in NPC tissues and cells. Loss-of-function revealed that NEAT1 knockdown inhibited proliferation and promoted apoptosis of NPC cells while gain-of-function revealed that upregulated NEAT1 showed an opposite effect. Moreover, NEAT1 was demonstrated to suppress miR-124 expression by direct interaction in NPC cells. Additionally, miR-124 reversed NEAT1-mediated pro-proliferation and anti-apoptosis effect. Furthermore, miR-124 regulated NPC cell proliferation and apoptosis via NF-κB signal pathway. Mouse models of NPC confirmed that NEAT1 overexpression facilitated tumor growth by modulating miR-124 in vivo. Taken together, this study indicated that upregulated NEAT1 promoted the tumorigenesis and progression of NPC through regulating miR-124/NF-κB signaling pathway, suggesting an attractive therapy target for NPC patients.

STAT3-induced up-regulation of lncRNA NEAT1 as a ceRNA facilitates abdominal aortic aneurysm formation by elevating TULP3

“…For example, NEAT1 was reported to sponge miR-101 in non-small cell lung cancer [34], papillary thyroid carcinoma [35], hepatocellular carcinoma [36] and breast cancer [11]. Besides, NEAT1 sponged miR-124 in neuroblastoma [37], retinoblastoma [38], Alzheimer's disease [38] and nasopharyngeal carcinoma [39]. Furthermore, NEAT1 sponged miR-129 in esophageal squamous cell carcinoma [40] and hepatoblastoma [41].…”

<p>Long Non-Coding RNA-NEAT1 Promotes Cell Migration and Invasion via Regulating miR-124/NF-κB Pathway in Cervical Cancer</p>

“…LncRNA-NEAT1 functions as a ceRNA of miRNAs, which plays an important regulatory role in cancer by antagonizing miRNAs functions and modulating miRNAs targets. 20 For example, lncRNA-NEAT1 promotes cell growth and invasion through miR-211/HMGA2 axis in breast cancer. 36 Here, miR-124 was identified as a target of lncRNA-NEAT1.…”

“…16,17 LncRNAs can regulate the expression of target mRNAs by acting as molecular sponges for miRNAs, thereby influencing the development of tumor. 18,19 Cheng et al 20 have reported that lncRNA-NEAT1 promotes the progression of nasopharyngeal carcinoma by regulating miR-124/Nuclear factor-κB (NF-κB) pathway. However, the interaction between lncRNA-NEAT1 and miR-124 in CC remains unclear.…”

Integrative analysis of an lncRNA‑associated competing endogenous RNA network in human trabecular meshwork cells under oxidative stress

“…another study showed that neaT1 regulates the mir-107-mediated expression of cyclin-dependent kinase 6 in laryngeal squamous cell cancer (32). a number of studies have demonstrated that the expression of NEAT1 can be activated by NF-κB and p53, and activate PI3K/AKT pathways (33)(34)(35). However, the function of neaT1 in the pathogenesis of glaucoma has not yet been investigated.…”

“…When oxidative stress occurs, PI3K/Akt, NF-κB and p53 signaling pathways are directly activated by roS, thus causing damage to ocular structures (7,9). The roS regulate lncrnas, and some lncRNAs influence these pathways (33)(34)(35)(36)(37)(38)(39)(40). a total of 70 differentially expressed lncrnas were obtained, of which 24 participate in the lncrna-mirna-mrna interaction network.…”