Long noncoding RNA NEAT1 regulates the development of osteosarcoma through sponging miR‐34a‐5p to mediate HOXA13 expression as a competitive endogenous RNA

Ji, Shaolin; Wang, Shunsheng; Zhao, Xiaodan; Lv, Li

doi:10.1002/mgg3.673

Cited by 21 publications

(17 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…lncRNAs have been reported to sponge miRNAs via a competitive endogenous mechanism, thus antagonizing the function of miRNAs ( 52 ). NEAT1 upregulates Homeobox A13 by targeting miR-34a-5p and regulates the development of osteosarcoma ( 53 ). NEAT1 also promotes cervical cancer cell proliferation by sponging miR-9-5p ( 13 ).…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA NEAT1 promotes ovarian cancer cell invasion and migration by interacting with miR‑1321 and regulating tight junction protein 3 expression

et al. 2020

View full text Add to dashboard Cite

Previous studies have reported that long non-coding RNAs (lncRNAs) have a significant role in the metastasis of tumors, including ovarian cancer (OC). The aim of the present study was to demonstrate the function and working mechanism of lncRNA nuclear enriched abundant transcript 1 (NEAT1) in OC. The expressions of NEAT1 in OC were measured by reverse transcription-quantitativePCR (RT-qPCR). The effects of NEAT1 on cell proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) were detected by Cell Counting Kit-8, transwell and wound healing assays, and western blotting. Dual-luciferase reporter assays were performed to confirm the correlated between NEAT and miR-1321, miR-1321 and TJP3. The effect of NEAT1 on miR-1321 and TJP3 was confirmed by RT-qPCR and western blotting. Elevated expression of NEAT1 was observed in OC cell lines, and NEAT1 expression was found to be positively related to the expression of tight junction protein 3 (TJP3), which is important in cancer development. Moreover, the present results indicated that NEAT1 and TJP3 expression levels were negatively correlated with microRNA (miR)-1321 expression in OC. Knockdown of NEAT1 attenuated the migration and invasion of OC cells, as well as increased miR-1321 expression and in turn led to the reduction of TJP3. Thus, the present study demonstrated that NEAT1 regulates TJP3 expression by sponging miR-1321 and enhances the epithelial-mesenchymal transition, invasion and migration of OC cells. Overall, the present study identified the function and mechanism of NEAT1 in OC, suggesting that NEAT1 may be a promising therapeutic target for OC metastasis.

show abstract

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA NEAT1 promotes ovarian cancer cell invasion and migration by interacting with miR‑1321 and regulating tight junction protein 3 expression

et al. 2020

View full text Add to dashboard Cite

show abstract

“… 28 Dysregulated lncRNAs have been confirmed to be relevant to the disease development. 29 Recent studies suggested that lncRNAs might serve as effective therapeutic targets for OS treatment. 16 , 30 , 31 In this study, we found that lncRNA HCG11 was highly expressed in OS related to the counterparts.…”

Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNA HCG11 Aggravates Osteosarcoma Carcinogenesis via Regulating the microRNA-579/MMP13 Axis

Wang¹,

Zhou²,

Zhang³

et al. 2020

IJGM

View full text Add to dashboard Cite

Background: Previous studies have suggested that long non-coding RNAs (lncRNAs) were involved in tumorigenesis of various human carcinomas, including osteosarcoma (OS). However, the expression and specific role of lncRNA HLA complex group 11 (HCG11) in OS remain unknown. The current study aimed at revealing the role of lncRNA HCG11 and its related mechanism in OS. Methods: lncRNA HCG11 expression was verified with RT-qPCR followed by sublocalization determination. LncRNA-microRNA (miRNA) and miRNA-mRNA interactions were predicted by online bioinformatics websites. Validation was performed using dualluciferase reporter gene assays, and gain-and loss-of-function experiments. The effects of lncRNA HCG11, miR-579 and matrix metalloproteinase 13 (MMP13) on the proliferation, migration and invasion, epithelial-mesenchymal transition (EMT) of OS cells were detected using cell counting kit-8 (CCK-8), Transwell assays and Western blot analysis. Results: LncRNA HCG11 overexpression was observed in OS tissues and cell lines. Downregulation of lncRNA HCG11/MMP13 or overexpression of miR-579 blocked the progression of OS cells. LncRNA HCG11, which is located in the cytoplasm, promoted MMP13 expression through sponging miR-579. Conclusion: LncRNA HCG11 might be beneficial for OS aggravation via sponging miR-579 and facilitating MMP13 expression, which represents a candidate biomarker and target for OS therapy.

show abstract

“…The cancer's large tumor heterogeneity and its low prevalence make it tough to gain meaningful progress in patient's survival [28]. Dysregulated lncRNAs have been confirmed to be relevant to the disease development [29]. Recent studies suggested that lncRNAs might serve as effective therapeutic targets for OS treatment [15,18,30].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, different studies indicated that lncRNA NEAT1 dysregulation boosted tumorigenesis in a variety of human malignancies, including OS. For example, Ji et al found that lncRNA NEAT1 participated in the development of OS as a ceRNA sponging to miR-34a-5p and thus regulate HOXA13 expression [29].…”

Section: Discussionmentioning

confidence: 99%

“…Through isolation of cytoplasm and nucleus, we confirmed that lncRNA NEAT1 was dominantly distributed in the cell cytoplasm, indicating that lncRNA NEAT1 might serve as a ceRNA. It is wellknown that abnormal expression of lncRNAs could work as ceRNAs for miRNA so as to modulate tumor progression [29]. Subsequently, bioinformatics and dual luciferase reporter gene assay clarified the binding relation between lncRNA NEAT1 and miR-579.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Long noncoding RNA NEAT1 aggravates osteosarcoma carcinogenesis via regulating the microRNA-579/MMP13 axis

Wang

Zhou

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Previous studies have suggested that long non-coding RNAs (lncRNAs) were involved in tumorigenesis in various human carcinomas, including osteosarcoma (OS). However, the expression and specific role of lncRNA NEAT1 in OS remain unknown. The current study aimed at revealing the role of lncRNA NEAT1 and its related mechanism in OS.Methods: Expression profiles of lncRNAs in OS tissues were constructed, and lncRNA NEAT1 expression was verified with RT-qPCR followed by sub-localization. LncRNA-microRNA (miRNA) and miRNA-mRNA interactions were predicted. Validation was performed using dual luciferase reporter gene assay, and gain-and loss-of-function experiments. The effects of lncRNA NEAT1, miR-579 and MMP13 on the proliferation, migration and invasion, epithelial-mesenchymal transition (EMT) of OS cells were detected using colony formation, cell counting kit-8 (CCK-8), Transwell assays and Western blot analysis. Results: LncRNA NEAT1 overexpression was observed in OS tissues and cell lines which located in the cytoplasm. Transfection-induced downregulation of lncRNA NEAT1/MMP13 or overexpression of miR-579 blocked the progression of OS cells. LncRNA NEAT1 promotes MMP13 through sponging miR-579.Conclusion: LncRNA NEAT1 might be beneficial for OS aggravation via sponging miR-579 and facilitating MMP13 expression, which represents a candidate marker and target for OS therapy.clarify the invisible mechanism underlying tumorigenesis and to develop novel molecular targets of OS.Long non-coding RNAs (lncRNAs), surpassing over 200 nucleotides in length, are a prominent class of RNAs exerting a crucial regulatory potential in tumor cell behaviors [6]. Increasing evidence has illustrated that aberrantly expressed lncRNAs were highly associated with the occurrence and development of many human malignancies, including OS [7-9]. Nuclear enriched abundant transcript 1 (NEAT1) is a lncRNA transcribed from the diverse endocrinal neoplasia locus [10]. The interaction of lncRNA NEAT1 has been documented in glioma, prostate cancer and lung adenocarcinoma [11][12][13].LncRNA NEAT1 was also suggested as a tumor promotor in OS by several studies [14][15][16].MicroRNAs (miRNAs) are tiny (~ 22 nucleotides) endogenous non-coding RNA molecules which act at the post-transcriptional level and regulate mRNA expression [17]. They are involved in multiple complicated cellular behaviors [18]. Importantly, emerging evidence has pointed out that miRNAs participate in OS, such as miR-18a-5p, miR-671-5p, miR-1301, and miR-212 [19-22]. MiR-579 was reported to be associated with malignancies, such as breast cancer, ovarian cancer, and human glioblastoma [23][24][25]. However, its function in OS deserves further studies.LncRNAs are proved to serve as competing endogenous RNAs (ceRNAs), which sponges certain miRNAs to mediate its target gene, thus changing the post-transcriptional regulation [1]. However, the concrete influence that lncRNA NEAT1 and miR-579 exerts on OS, especially their interaction is still to be elucidated. In this experimen...

show abstract

Long noncoding RNA NEAT1 regulates the development of osteosarcoma through sponging miR‐34a‐5p to mediate HOXA13 expression as a competitive endogenous RNA

Cited by 21 publications

References 31 publications

Long non‑coding RNA NEAT1 promotes ovarian cancer cell invasion and migration by interacting with miR‑1321 and regulating tight junction protein 3 expression

Long non‑coding RNA NEAT1 promotes ovarian cancer cell invasion and migration by interacting with miR‑1321 and regulating tight junction protein 3 expression

Long Non-Coding RNA HCG11 Aggravates Osteosarcoma Carcinogenesis via Regulating the microRNA-579/MMP13 Axis

Long noncoding RNA NEAT1 aggravates osteosarcoma carcinogenesis via regulating the microRNA-579/MMP13 axis

Contact Info

Product

Resources

About