Long noncoding RNA NR2F1-AS1 enhances the malignant properties of osteosarcoma by increasing forkhead box A1 expression via sponging of microRNA-483-3p

Li, Shenglong; Zheng, Ke; Pei, Yi; Wang, Wei; Zhang, Xiaojing

doi:10.18632/aging.102563

Cited by 38 publications

(36 citation statements)

References 37 publications

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“…Therefore, these data demonstrated that NR2F1-AS1 facilitates the OS cell malignant phenotype via downregulating miR-485-5p and miR-218-5p, and then upregulating BIRC5 expression. Notably, NR2F1-AS1 was reported to enhance the malignant properties of OS by increasing FOXA1 expression via sponging of microRNA-483-3p (16). In our manuscript, we revealed that NR2F1-AS1 acted as an oncogene in osteosarcoma that facilitated osteosarcoma cell proliferation and migration through targeting miR-485-5p and miR-218-5p and then targeting BIRC5.…”

Section: Discussionmentioning

confidence: 67%

“…In addition, in endometrial cancer, NR2F1-AS1 has been reported to sponge miR-363 and target SOX4 to play a role in cell proliferation and migration (15). Notably, it has been validated that NR2F1-AS1 facilitates the malignant properties of OS via binding to miR-483-3p to enhance forkhead box A1 (FOXA1) expression (16). These findings demonstrate that NR2F1-AS1 acts as an oncogene in cancer.…”

Section: Introductionmentioning

confidence: 85%

“…In hepatocellular carcinoma, DLGAP1-AS1 has been confirmed to facilitate cell proliferation via the miR-486-5p/H3F3B axis (35). NR2F1-AS1 has been reported to play a role in papillary thyroid carcinoma (14), ESCC (12), endometrial cancer (15), hepatocellular carcinoma (36) and OS (16). In OS, NR2F1-AS1 has been identified to sponge miR-483-3p to upregulate FOXA1 and enhance the malignant properties of OS cells (16).…”

Section: Discussionmentioning

confidence: 99%

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Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis

Jia

Wang

et al. 2020

Oncol Rep

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Long non-coding RNA (lncRNA) NR2F1 antisense RNA 1 (NR2F1-AS1) has been reported to be an oncogene in several cancer types, including osteosarcoma (OS). However, the underlying fundamental molecular mechanism of NR2F1-AS1 in OS remains largely unknown, which the present study aimed to elucidate. The present study demonstrated that NR2F1-AS1 expression is markedly increased in OS, and NR2F1-AS1 was shown to exert oncogenic functions in OS. Further molecular mechanistic studies revealed that microRNA (miR)-485-5p and miR-218-5p were direct targets of NR2F1-AS1. More importantly, miR-485-5p and miR-218-5p exhibited low expression levels and were negatively correlated with NR2F1-AS1 expression in OS tissues. It was then identified that baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5) was a direct target of miR-485-5p and miR-218-5p in OS cells. Furthermore, a series of experiments suggested that NR2F1-AS1 affects the proliferation, migration, invasion and apoptosis of OS cells by regulating BIRC5. Finally, it was revealed that silencing of NR2F1-AS1 repressed the OS cell malignant phenotype by binding with miR-485-5p and miR-218-5p, and then downregulating BIRC5 expression, which suggests that the NR2F1-AS1/miR-485-5p/miR-218-5p/BIRC5 axis could be a potential target for treating OS.

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Section: Discussionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

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Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis

Jia

Wang

et al. 2020

Oncol Rep

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“…RT-qPCR was performed as described previously. 28 TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.) was employed for total-RNA extraction. The concentration and purity of total RNA were evaluated on a NanoDrop 2000 spectrophotometer (NanoDrop Technologies; Thermo Fisher Scientific, Inc.).…”

Section: Reverse-transcription Quantitative Polymerase Chain Reactionmentioning

confidence: 99%

Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression

Dai

Wei

Zhang

et al. 2020

CMAR

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Purpose: A long noncoding RNA called ZFPM2 antisense RNA 1 (ZFPM2-AS1) has been verified as a key modulator in multiple human cancer types. Nonetheless, the expression and functions of ZFPM2-AS1 in cervical cancer remain poorly understood. Therefore, our purpose was to characterize the expression pattern, clinical value, and detailed roles of ZFPM2-AS1 in cervical cancer. Methods: Reverse-transcription quantitative PCR was carried out to measure ZFPM2-AS1 expression in cervical cancer. A Cell Counting Kit-8 assay, flow cytometry, Transwell migration and invasion assays, and a tumor xenograft experiment were conducted to determine the influence of ZFPM2-AS1 on cervical cancer cell proliferation, apoptosis, migration, and invasion in vitro and on tumor growth in vivo, respectively. Results: ZFPM2-AS1 was found to be aberrantly upregulated in cervical cancer, and its upregulation was associated with unfavorable values of clinical parameters. A ZFPM2-AS1 knockdown significantly reduced cervical cancer cell proliferation, migration, and invasion and increased apoptosis in vitro. The ZFPM2-AS1 knockdown decelerated tumor growth of cervical cancer cells in vivo. Molecular investigation indicated that ZFPM2-AS1 acts as a molecular sponge of microRNA-511-3p (miR-511-3p) in cervical cancer cells. Fibroblast growth factor receptor 2 (FGFR2) mRNA was validated as a direct target of miR-511-3p in cervical cancer, and its expression was positively modulated by ZFPM2-AS1. The effects of the ZFPM2-AS1 knockdown on malignant characteristics of cervical cancer cells were greatly attenuated by miR-511-3p inhibition. Conclusion: ZFPM2-AS1 promotes cervical cancer progression through upregulation of miR-511-3p-FGFR2 axis output, thereby pointing to possible diagnostics and therapeutics based on the ZFPM2-AS1-miR-511-3p-FGFR2 pathway.

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“…19 LncRNA NR2F1-AS1 acts as a ceRNA in OS and enhances cell growth by targeting miR-483-3p/FOXA1 axis. 20 Previously, LINC01535 functioned as a ceRNA in cervical cancer and accelerated occurrence and development. 21 However, the function and molecular mechanism of LINC01535 in OS have not been studied.…”

Section: Introductionmentioning

confidence: 99%

LINC01535 Promotes the Development of Osteosarcoma Through Modulating miR-214-3p/KCNC4 Axis

Yao¹,

Wu²,

Gu³

et al. 2020

CMAR

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Background: Osteosarcoma (OS) is the most common primary bone tumor in group of children and adolescents. Increasing studies showed that long non-coding RNAs (lncRNAs) exerted important functions in the development of tumors, including OS. LINC01535 is an lncRNA which has been studied in cervical cancer but not in OS. Aim of the Study: This study was aimed to explore the biological function and mechanism of LINC01535 in OS. Methods: LINC01535 expression was detected by qRT-PCR. Colony formation assay, EdU assay and CCK-8 assay were applied to check cell proliferation ability in OS. Flow cytometry analysis was conducted to measure cell apoptosis capacity. Wound healing assay and transwell assay were performed to assess cell migration and invasion. Luciferase reporter assay and RNA pull-down assay were carried out to verify the molecular mechanism. Results: The high expression of LINC01535 was presented in OS tissues and cell lines compared with adjacent normal tissues and human osteoblasts. Moreover, OS patients with high LINC01535 expression exhibited poor prognosis. Loss-of-function assay revealed that silenced LINC01535 significantly attenuated cell proliferation, migration and invasion, and enhanced cell apoptosis in OS. Through mechanistic exploration, we found that LINC01535 interacted with miR-214-3p, and KCNC4 was validated to be a target gene of miR-214-3p. The levels of KCNC4 mRNA and protein were positively modulated by LINC01535 and reversely mediated by miR-214-3p. Based on rescue experiments, KCNC4 overexpression reserved the suppressive function of silenced LINC01535 on OS cell growth, migration and invasion. Conclusion: LINC01535, miR-214-3p and KCNC4 constituted an effective axis that exerted a pregnant regulation in OS development, which is a quite meaningful discovery for exploring potential therapeutic methods for OS patients.

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Long noncoding RNA NR2F1-AS1 enhances the malignant properties of osteosarcoma by increasing forkhead box A1 expression via sponging of microRNA-483-3p

Cited by 38 publications

References 37 publications

Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis

Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis

<p>Long Noncoding RNA <em>ZFPM2-AS1</em> Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression</p>

<p>LINC01535 Promotes the Development of Osteosarcoma Through Modulating miR-214-3p/KCNC4 Axis</p>

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