Long noncoding RNA PAGBC contributes to nitric oxide (NO) production by sponging miR‐511 in airway hyperresponsiveness upon intubation

Abstract

Background and Objectives In this study, we aimed to study the molecular mechanisms underlying the symptoms of hyperresponsiveness during intubation. Method The value of circulating long noncoding RNA (lncRNA)‐prognosis‐associated gallbladder cancer (PAGBC) in the prediction of hyperresponsiveness upon intubation during general anesthesia was evaluated via the receiver operating characteristic analyses of serum miR‐511, serum PAGBC, and serum nitric oxide (NO). In addition, the possible association between lncRNA‐PAGBC/NOS1 messenger RNA (mRNA) and miR‐511 was further validated via real‐time quantitative polymerase chain reaction, immunohistochemistry assay, computational analysis, and luciferase assay. Enzyme‐linked immunosorbent assay and Western blot analysis were also conducted to establish the regulatory relationship among PAGBC, miR‐511, and NO synthase 1 (NOS1). Results Compared with circulating miR‐511 and serum NO, circulating PAGBC was associated with a higher predictive value. In addition, a negative correlation was found between serum miR‐511 and serum PAGBC (multicorrelation coefficient: −0.5) as well as between serum miR‐511 and serum NO (multicorrelation coefficient: −0.6). In addition, both lncRNA‐PAGBC and NO were decreased in patients with hyperresponsiveness, whereas the levels of miR‐511 and NOS1 in these patients were similar to those in normal patients. Furthermore, our computational analyses and luciferase assays validated the direct binding between miR‐511 and lncRNA‐PAGBC, whereas NOS1 mRNA was identified as a virtual target gene of miR‐511. Moreover, in the presence of lncRNA‐PAGBC, we also observed an evident increase in the levels of NOS1 and NO accompanied by an obvious decrease of miR‐511 expression. Conclusion LncRNA‐PAGBC downregulated the expression of miR‐511, which in turn upregulated the expression of NOS1 mRNA and led to the increase in NOS1 expression, thus leading to the inhibited responsiveness (normal‐responsiveness rather than hyperresponsiveness) to intubation in patients.

Geniposide Alleviates Oxidative Stress of Mice With Depression-Like Behaviors by Upregulating Six3os1

“…In human beings, miR-511 was found to be highly expressed as compared with controls in dead basolateral amygdala from suicided individuals due to depressive attack (Maheu et al, 2015). Notably, another lncRNA prognosis-associated gallbladder cancer competitively bound to miR-511 in both hyperresponsiveness and human gallbladder cancer has been previously highlighted (Wu et al, 2017;Li et al, 2018), which indicated that miR-511 could be regulated by lncRNAs.…”

MiR-511 mimic transfection inhibits the proliferation, invasion of osteosarcoma cells and reduces metastatic osteosarcoma tumor burden in nude mice via targeting MAPK1

“…Researches revealed different targets of miR-511, such as AKT1, Insulin-like growth factor-1, Hepatomaderived growth factor, TLR4, NO synthase 1 and TRIM24 [5,18,[22][23][24][25]. In present study, the interaction has been predicted in targetscan (http://www.…”

“…A lot of studies have demonstrated the importance of miRNA in multiple human cancers [15]. miR-511 is considered as a tumor suppressor in multiple cancers including lung cancer, prostate cancer [16], gastric cancer [17], gallbladder cancer [18,19], hepatocellular carcinoma [20] and colorectal cancer [5] and only few reports showed that miR-511 served as a carcinogenic factor in other cancers, such as HCV-associated diffuse large B-cell lymphoma as well as in human blood monocyte-derived dendritic cells and macrophages [21]. Interestingly, our study revealed that miR-511 was remarkably downregulated in osteosarcoma MG63, SaOS2 and U2OS cells.…”