Long Noncoding RNA PVT1 Facilitates Cervical Cancer Progression via Negative Regulating of miR-424

Gao, Yali; Zhao, Zishen; Zhang, Mingyun; Liang, Han; Dong, Yaping; Xu, Bo

doi:10.3727/096504017x14881559833562

Cited by 125 publications

(89 citation statements)

References 18 publications

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“…Clinically, the aberrant overexpression of CASC9 was correlated with the clinical of glioma patients, implying the tumour promoting biomarker of CASC9. More than CASC9, numerous of lncRNAs have been identified to be aberrantly overexpressed in human tumourous tissue, acting as the oncogenic molecular, such as PVT1, UCA1, CCAT2 et al Similar to this finding, our results also discover this worthy conclusion.…”

Section: Discussionsupporting

confidence: 85%

Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis

Liu

Yang

et al. 2018

J Cellular Molecular Medi

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Emerging evidence have illustrated the vital roles of long noncoding RNAs (lncRNAs) in glioma. Nevertheless, the majority of their roles and mechanisms in gliomagenesis are still largely unclear. In this study, we investigate the roles of lncRNA CASC9 on glioma tumourigenesis and authenticate its potential mechanisms. Results manifested that CASC9 was highly expressed in glioma specimens and cells, moreover, the ectopic overexpression was correlated with glioma patients’ clinic. Functional studies found that siRNA‐mediated CASC9 silencing inhibited the proliferative ability, invasion in vitro, and impaired the tumour growth in vivo. Mechanical studies revealed that miR‐519d both targeted the 3′‐UTR of CASC9 and STAT3 mRNA, which was identified by luciferase reporter assay and RNA immunoprecipitation (RIP). Moreover, chromatin immunoprecipitation (ChIP) and luciferase reporter assay revealed that STAT3, an oncogenic transcription factor, could bind with the promoter of CASC9 and activate its transcriptional level. In conclusion, our results concluded that CASC9 promotes STAT3 expression via sponging miR‐519d, in return, STAT3 activate CASC9 transcription, forming a positive feedback loop of CASC9/miR‐519d/STAT3. The novel finding provides a potential therapeutic target for glioma.

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Section: Discussionsupporting

confidence: 85%

Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis

Liu

Yang

et al. 2018

J Cellular Molecular Medi

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“…Various miRNA have been demonstrated to be involved in a variety of cellular biological processes, including cell proliferation, differentiation, survival, migration and invasion (8,(11)(12)(13). Additionally, a large number of miRNA, including miR-181 (14), -186 (15), -126 (16) and -424 (17), have been reported to serve promoting or suppressive roles in tumor growth and metastasis of various human malignances, such as cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

Jiang

Hou

et al. 2018

Exp Ther Med

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Abstract. MicroRNA serve crucial roles in a variety of human cancer types. The miR-302-367 cluster has been reported to suppress the proliferation of cervical carcinoma cells through the novel target AKT1; however, the molecular mechanism of miR-302 in cervical cancer metastasis remains unclear. The present study aimed to investigate the clinical significance of miR-302-3p expression in cervical cancer, and to examine the regulatory mechanism of miR-302-3p in the malignant phenotypes of cervical cancer cells. The present data indicated that miR-302-3p was significantly downregulated in cervical cancer tissues compared with the level in adjacent non-tumor tissues, and low expression of miR-302-3p was significantly associated with node metastasis, advanced clinical stage, and poor prognosis in patients with cervical cancer. Restoration of miR-302-3p expression caused a significant reduction in cervical cancer cell migration and invasion. Defective in cullin neddylation 1 domain containing 1 (DCUN1D1) was identified as a novel target gene of miR-302-3p, and miR-302-3p negatively regulated the mRNA and protein expression of DCUN1D1 in cervical cancer HeLa cells. Additionally, overexpression of DCUN1D1 rescued the effects of miR-302-3p on the migration and invasion of cervical cancer cells. Furthermore, DCUN1D1 was upregulated in cervical cancer tissues compared with the levels in adjacent tissues, and its high expression was associated with node metastasis, advanced clinical stage, and shorter survival time in patients with cervical cancer. Notably, a negative correlation between miR-302-3p and DCUN1D1 expression in cervical cancer tissues was observed. Taken together, the present study suggests that miR-302-3p serves a suppressive role in cervical cancer metastasis, partly at least, via directly targeting DCUN1D1. Therefore, miR-302-3p/DCUN1D1 may be a potential therapeutic target for cervical cancer treatment.

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“…Among the candidate biomarkers we obtained, some of which have been confirmed, might serve as fingerprint for CC diagnosis. For example, serum PVT1 level is significantly increased in CC patients and correlated with tumor size, clinical stage, and lymph node metastasis of CC [26]. Circulating PVT1 could be used as a novel noninvasive biomarker for CC with the AUC range from 0.758 to 0.892 in different cohort [27].…”

Section: Discussionmentioning

confidence: 99%

Four Circulating Long Non-Coding RNAs Act as Biomarkers for Predicting Cervical Cancer

Sun

Wang

Zhao

et al. 2018

Gynecol Obstet Invest

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Background/Aims: Circulation long non-coding RNAs (lncRNAs) have emerged recently as major players in tumor biology and might be applied for cancer diagnosis, prognosis, or potential therapeutic targets. In this study, we aimed to explore whether the circulation lncRNA could predict the tumorigenesis of surgical squamous cervical cancer (CC). Methods: In this study, we applied the lncRNA microarray to screen the potential biomarker for CC. Real-time quantitate polymerase chain reaction was conducted for further validation in a larger sample size. The multi-stage validation and risk score formula analysis were used to examine the sensitivity and specificity. Results: We discovered 4 lncRNAs including HOTAIR, PVT1, XLOC_000303, and AL592284.1, which were upregulated in CC comparing with the cancer-free controls. Further, the receiver operating characteristic curve (ROC) analysis by risk score formula revealed the combined 4 factors with a high diagnostic ability with the area under ROC curve value of 0.875 and 0.958 in training set and validation set respectively. We finally confirmed the stable detection of the 4 lncRNAs by 5 cycles of freezing and thawing. Conclusion: HOTAIR, PVT1, XLOC_000303, and AL592284.1 might be the potential biomarkers for predicting the tumorigenesis of CC in the future.

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Long Noncoding RNA PVT1 Facilitates Cervical Cancer Progression via Negative Regulating of miR-424

Cited by 125 publications

References 18 publications

Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis

Long noncoding RNA CASC9/miR‐519d/STAT3 positive feedback loop facilitate the glioma tumourigenesis

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

Four Circulating Long Non-Coding RNAs Act as Biomarkers for Predicting Cervical Cancer

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