Long noncoding RNA PVT1 promotes tumour progression via the miR-128/ZEB1 axis and predicts poor prognosis in esophageal cancer

Hu, Jing; Gao, Weifang

doi:10.1016/j.clinre.2021.101701

Cited by 14 publications

(9 citation statements)

References 17 publications

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“…The up-regulation of XIST expression promoted the proliferation of ESCC cells [ 48 ]. Besides, PVT1 and NEAT1 were also verified to be related to ESCC [ 49 , 50 , 51 , 52 ]. BCYRN1 has not been confirmed to be associated with ESCC.…”

Section: Resultsmentioning

confidence: 99%

MSF-UBRW: An Improved Unbalanced Bi-Random Walk Method to Infer Human lncRNA-Disease Associations

Dai¹,

Zhu²,

Liu³

et al. 2022

Genes

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Long-non-coding RNA (lncRNA) is a transcription product that exerts its biological functions through a variety of mechanisms. The occurrence and development of a series of human diseases are closely related to abnormal expression levels of lncRNAs. Scientists have developed many computational models to identify the lncRNA-disease associations (LDAs). However, many potential LDAs are still unknown. In this paper, a novel method, namely MSF-UBRW (multiple similarities fusion based on unbalanced bi-random walk), is designed to explore new LDAs. First, two similarities (functional similarity and Gaussian Interaction Profile kernel similarity) of lncRNAs are calculated and fused linearly, also for disease data. Then, the known association matrix is preprocessed. Next, the linear neighbor similarities of lncRNAs and diseases are calculated, respectively. After that, the potential associations are predicted based on unbalanced bi-random walk. The fusion of multiple similarities improves the prediction performance of MSF-UBRW to a large extent. Finally, the prediction ability of the MSF-UBRW algorithm is measured by two statistical methods, leave-one-out cross-validation (LOOCV) and 5-fold cross-validation (5-fold CV). The AUCs of 0.9391 in LOOCV and 0.9183 (±0.0054) in 5-fold CV confirmed the reliable prediction ability of the MSF-UBRW method. Case studies of three common diseases also show that the MSF-UBRW method can infer new LDAs effectively.

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Section: Resultsmentioning

confidence: 99%

MSF-UBRW: An Improved Unbalanced Bi-Random Walk Method to Infer Human lncRNA-Disease Associations

Dai¹,

Zhu²,

Liu³

et al. 2022

Genes

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“…This indicated that LATS1 and YAP1 are downstream effectors of PVT1 , and YAP1 has a positive feedback effect on PVT1 ( 26 ). Hu et al ( 27 ) suggested that PVT1 promotes the downstream expression of ZEB1 and E-cadherin via miR-128, promoting ESCC progression.…”

Section: Long-chain Non-coding Rnas Promote the Occurrence And Develo...mentioning

confidence: 99%

“…Multiple LncRNAs are also differentially expressed in serum in patients with cancer. Hu et al ( 27 ) analyzed sera from 76 ESCC patients and found that serum PVT1 levels were related to lymph node metastasis, TNM staging, and postoperative metastasis. High levels of PVT1 often indicate a worse overall survival, suggesting that PVT1 could be used as a valuable serum marker for ESCC diagnosis and prognostication.…”

Section: Liquid Biopsy Long-chain Non-coding Rna Is Conducive To the ...mentioning

confidence: 99%

Long Non-Coding RNA in Esophageal Cancer: A Review of Research Progress

Yang¹,

Chen²

2022

Pathol. Oncol. Res.

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In recent years, there has been significant progress in the diagnosis and treatment of esophageal cancer. However, owing to the lack of early diagnosis strategies and treatment targets, the prognosis of patients with esophageal cancer remains unsatisfactory. There is an urgent need to identify novel biomarkers and treatment targets for esophageal cancer. With the development of genomics, long-chain non-coding RNAs (LncRNAs), which were once considered transcriptional “noise,” are being identified and characterized rapidly in large numbers. Recent research shows that LncRNAs are closely related to a series of steps in tumor development and play an important regulatory role in DNA replication, transcription, and post-transcriptional regulation. The abnormal expression of LncRNAs leads to tumor cell proliferation, migration, invasion, and treatment resistance. This review focuses on the latest progress in research on the abnormal expression and functional mechanisms of LncRNAs in esophageal cancer. Further, it discusses the potential applications of these findings towards achieving an early diagnosis, improving treatment efficacy, and evaluating the prognosis of esophageal cancer.

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“…For example, lncRNA POU3F3, which is upregulated in EC, facilitates radioresistance and expression of cancer stem cell markers in EC cells 8 . A high serum level of lncRNA PVT1 is related to poor prognosis and postoperative metastasis in EC 9 . The lncRNA cyclin‐dependent kinase inhibitor 2 B antisense RNA 1 (CDKN2B‐AS1, also referred to as antisense noncoding RNA in the INK4 locus) exerts a procarcinogenic effect in nasopharyngeal carcinoma, osteosarcoma, and renal cell carcinoma 10–12 .…”

Section: Introductionmentioning

confidence: 99%

“…8 A high serum level of lncRNA PVT1 is related to poor prognosis and postoperative metastasis in EC. 9 The lncRNA cyclindependent kinase inhibitor 2 B antisense RNA 1 (CDKN2B-AS1, also referred to as antisense noncoding RNA in the INK4 locus) exerts a procarcinogenic effect in nasopharyngeal carcinoma, osteosarcoma, and renal cell carcinoma. [10][11][12] Moreover, gene-level analyses have shown that CDKN2B-AS1 is associated with ESCC.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA CDKN2B‐AS1 silencing protects against esophageal cancer cell invasion and migration by inactivating the TFAP2A/FSCN1 axis

Zhu

Xue

Jiang

et al. 2022

The Kaohsiung J of Med Scie

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Esophageal cancer (EC) is the most aggressive malignancy in the gastrointestinal tract.Long noncoding RNA cyclin-dependent kinase inhibitor 2 B antisense RNA 1 (CDKN2B-AS1) is implicated in EC development. However, the specific mechanisms involved remain poorly defined. Therefore, this research aimed to explore the mechanism of action of CDKN2B-AS1 in EC. Quantitative real-time polymerase chain reaction was conducted to measure CDKN2B-AS1 expression in EC cells and western blotting was utilized to evaluate transcription factor AP-2 alpha (TFAP2A) and fascin actin-bundling protein 1 (FSCN1) expression. After gain-of-function and loss-of-function assays, cell proliferation, migration, invasion, apoptosis, and apoptosis-related protein expression were assessed using cell counting kit-8, scratch tests, Transwell assays, flow cytometry, and western blotting, respectively. The binding relationship between CDKN2B-AS1 and TFAP2A was assessed by RNA immunoprecipitation and RNA pull-down assays. The binding relationship between TFAP2A and FSCN1 was evaluated using dual-luciferase reporter and chromatin immunoprecipitation assays. Tumor xenografts from nude mice were used for in vivo verification. CDKN2B-AS1, TFAP2A, and FSCN1 were upregulated in EC cells. Mechanistically, CDKN2B-AS1 transcriptionally activated FSCN1 by recruiting TFAP2A to the FSCN1 promoter. Silencing CDKN2B-AS1 or TFAP2A suppressed EC cell proliferative, migrating, and invasive properties and augmented apoptosis. TFAP2A was bound to CDKN2B-AS1 and the FSCN1 promoter. Overexpression of TFAP2A or FSCN1 abolished the effects of CDKN2B-AS1-silencing on EC cell function. CDKN2B-AS1 silencing curtailed tumorigenesis in nude mice, which was nullified by the upregulation of TFAP2A or FSCN1. Our findings demonstrated the antioncogenic effects of silencing CDKN2B-AS1 in EC through inactivation of the TFAP2A/FSCN1 axis.

show abstract

Long noncoding RNA PVT1 promotes tumour progression via the miR-128/ZEB1 axis and predicts poor prognosis in esophageal cancer

Cited by 14 publications

References 17 publications

MSF-UBRW: An Improved Unbalanced Bi-Random Walk Method to Infer Human lncRNA-Disease Associations

MSF-UBRW: An Improved Unbalanced Bi-Random Walk Method to Infer Human lncRNA-Disease Associations

Long Non-Coding RNA in Esophageal Cancer: A Review of Research Progress

Long noncoding RNA CDKN2B‐AS1 silencing protects against esophageal cancer cell invasion and migration by inactivating the TFAP2A/FSCN1 axis

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