Long noncoding RNA SNHG10 promotes colorectal cancer cells malignant progression by targeting miR-3690

Abstract

Long noncoding RNA small nucleolar RNA host gene 10 (SNHG10) has been suggested to function as tumor promoter in various human cancer types. Herein, the role of SNHG10 in colorectal cancer (CRC) was explored. Expression levels of genes in colorectal cancer tissues and cell lines were detected by Starbase and reverse transcription quantitative PCR (RT-qPCR) Cell Counting Kit-8 (CCK-8), the BrdU incorporation assay and Transwell assays were explored to study the function of SNHG10 in HCT116 and DXH-1 cells. In addition, the interaction of SNHG10 and miR-3690 was analyzed by dual-luciferase reporter assays. SNHG10 had a high expression level in CRC tissues and cell lines. Meanwhile, knockdown of SNHG10 reduced cell viability, inhibited cell proliferation and decreased cell migration and invasion. Moreover, bioinformatics analysis revealed that one potential target gene of SNHG10 was miR-3690. Dual-luciferase reporter assay confirmed that miR-3690 directly targeted SNHG10. Importantly, SNHG10 could decrease the expression of miR-3690 in HCT116 and DXH-1 cells. More importantly, the silencing of miR-3690 reversed the effect of the SNHG10 knockdown on the cell viability, proliferation, migration and invasion of HCT116 and DXH-1 cells. The present results demonstrated that SNHG10 promotes colorectal cancer cells the malignant progression by targeting miR-3690.

RETRACTED ARTICLE: Knockdown of small nucleolar RNA host gene 10 (SNHG10) alleviates the injury of human neuroblastoma cells via the miR-1277-5p/insulin substrate receptor 2 axis

“…Recently many lncRNAs have been implicated in regulation of the pathogenesis of Parkinson’s disease [ 24 ]. LncRNA SNHG10 lncRNA was previously reported to contribute to colorectal cancer cells progression by targeting miR-3690 [ 25 ]. However, the potential roles of SNHG10 in Parkinson’s disease remain unknown.…”

Identification and Validation of Apparent Imbalanced Epi-lncRNAs Prognostic Model Based on Multi-Omics Data in Pancreatic Cancer

“…Abnormally expressed lncRNAs are key regulators of important biological functions in cancer cells ( Harries, 2012 ). For example, linc01207 regulates ARHGAP11A and promotes the progression of non-small cell lung cancer by secreting mir-525-5p ( Zhang et al, 2022 ); long noncoding RNA, SNHG10, promotes the malignant progression of colorectal cancer cells by targeting mir-3690 ( Zhang et al, 2021a ). The important functions of lncRNAs are also described in pancreatic cancer.…”

“…LINC00941 regulates VEGFA expression by adsorbing mir-877-3p and promotes the progression of non-small cell lung cancer ( Ren et al, 2021 ). SNHG10 can promote tumor progression in colorectal cancer ( Zhang et al, 2021a ) and acute myoid leukemia (AML) through microRNA interaction ( Xiao et al, 2021 ). However, as compared to non-tumor tissues, the expression of SNHG10 in non-small cell lung cancer (NSCLC) is down-regulated, and its underlying mechanism could be the regulation of miR-21 gene methylation to enhance NSCLC cell proliferation ( Liang et al, 2020 ).…”

Circular RNA circPRKDC promotes tumorigenesis of gastric cancer via modulating insulin receptor substrate 2 (IRS2) and mediating microRNA-493-5p

“…Dual-luciferase reporter assay (Promega Corporation) was performed to investigate the interaction between miR-493-5p and circPRKDC or IRS2 [ 24 ]. Site-directed mutagenesis was used to create the mutant 3ʹ-untranslated region (UTR) sequence of IRS2 and circPRKDC.…”