Long noncoding RNA XIST participates in bladder cancer by downregulating p53 via binding to TET1

Hu, Bo; Shi, Guowei; Li, Qian; Li, Wei; Zhou, Hui

doi:10.1002/jcb.27920

Cited by 31 publications

(22 citation statements)

References 32 publications

(80 reference statements)

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“…Compared with hepatocellular carcinoma, our results indicated that increased TET1 expression induced apoptosis and inhibited the growth of U2OS cells, consistent with previous data obtained from colon cancer cells [27]. TET1 expression was recently reported to be associated with apoptosis in bladder cancer cells [28]. As shown in the present study, TET1 overexpression induced apoptosis in HU-treated cells.…”

Section: Discussionsupporting

confidence: 93%

Hydroxyurea promotes TET1 expression and induces apoptosis in osteosarcoma cells

Teng

et al. 2019

Bioscience Reports

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Ten-eleven translocation (TET) proteins are abnormally expressed in various cancers. Osteosarcoma cells were treated with hydroxyurea to investigate the expression pattern of TET proteins in these cells. The expression of TET1 was increased in U2OS cells after treatment with hydroxyurea. In addition, hydroxyurea increased cell apoptosis and altered the cell cycle. TET proteins catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC); therefore, 5mC and 5hmC levels were evaluated. Increased 5hmC levels were observed after the hydroxyurea treatment. Experiments examining cell apoptosis and the cell cycle after knockdown and overexpression of TET1 were conducted to further investigate whether TET1 expression affected cell growth. The overexpression of TET1 increased cell apoptosis and inhibited cell growth. Taken together, TET1 expression regulated proliferation and apoptosis in U2OS cells, changes that were associated with 5hmC levels.

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Section: Discussionsupporting

confidence: 93%

Hydroxyurea promotes TET1 expression and induces apoptosis in osteosarcoma cells

Teng

et al. 2019

Bioscience Reports

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“…MAGI2-AS3 (ENST00000452320) was observed in hepatocellular carcinoma ( 52 ). Notably, it has been reported that lncRNA XIST ( 53 ), MEG3 ( 54 ), MAGI2-AS3 ( 55 ), FENDRR and ADAMTS9-AS2 ( 56 ) act as prognosis markers of patients with BC or exert significant roles in BC progression through different cancer-associated pathways. For instance, Kouhsar et al ( 56 ) revealed that based on the analysis of the ceRNA networks in the early stage of BC, the lncRNAs FENDRR, ADAMTS9-AS2 and CARMN may regulate MEG3 in NMIBC via harboring some critical miRNAs such as miR-143-3p, miR-34a-3p and miR-106a-5p.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNAs, ENST00000598996 and ENST00000524265, are correlated with favorable prognosis and act as potential tumor suppressors in bladder cancer

Shen

Wang

et al. 2020

Oncol Rep

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Bladder cancer (BC) is a serious malignancy worldwide due to its distant metastasis and high recurrence rates. Increasing evidence has indicated that dysregulated long non-coding RNAs (lncRNAs) are involved in tumorigenesis and progression in multiple malignancies. However, their clinical significances, biological functions and molecular mechanisms in BC remain poorly understood. Hence, the present study investigated the expression profile of lncRNAs and mRNAs in five BC tissues and the corresponding adjacent normal specimens using high-throughput RNA sequencing (RNA-seq). A total of 103 differentially expressed (DE) lncRNAs were identified, including 35 upregulated and 68 downregulated ones in BC tissues. Similarly, a total of 2,756 DE-mRNAs were detected, including 1,467 upregulated and 1,289 downregulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses, and lncRNA-miRNA-mRNA network analyses suggested that these dysregulated lncRNAs are potentially implicated in the onset and progression of BC. Subsequently, four lncRNAs (upregulated ENST00000433108; downregulated ENST00000598996, ENST00000524265 and ENST00000398461) and two mRNAs (upregulated CCNB1 and CDK1) in 64 pairs of BC and adjacent normal tissues and four BC cell lines were detected using reverse transcription-quantitative PCR and these results were consistent with the sequencing data. Additionally, Fisher's exact test, Kaplan-Meier plots, and Cox regression analyses were used for elucidating the clinical values of ENST00000598996 and ENST00000524265. Furthermore, a receiver operating characteristic curve was constructed to assess their diagnostic values. The low expression level of ENST00000598996 and ENST00000524265 was correlated with unfavorable clinicopathological parameters, and shorter progression-free and overall survival time, whereas, ENST00000433108 was not associated with either. The in vitro functional experiments also revealed that the overexpression of ENST00000598996 and ENST00000524265 decreased the proliferation, migration, and invasion abilities of BC cells. Collectively, the results of the present study provide a novel landscape of lncRNA and mRNA expression profiles in BC. In addition, the results also indicated that ENST00000598996 and ENST00000524265 may serve as tumor suppressors, potential diagnostic biomarkers and prognostic predictors for patients with BC.

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“…Reportedly, XIST has been shown to be associated with proliferation, apoptosis, tumor development, metastasis, patient prognosis, and response to chemo-and radio-therapies of cancer. In bladder cancer cells in vitro, targeting XIST suppressed cell invasion and proliferation by downregulation of p53 [30]. In nasopharyngeal carcinoma (NPC), targeting XIST inhibited NPC cell proliferation and invasion in vitro and NPC tumor growth in vivo [31].…”

Section: Discussionmentioning

confidence: 99%

Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal

Gao

Chen

et al. 2019

Bioengineered

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The long noncoding RNA X-inactive specific transcript (XIST) plays vital roles in tumor progression. However, the underlying mechanisms remain unclear. This study investigated the effects and mechanisms of targeting XIST on osteosarcoma (OS) cells in vitro and in vivo. We used shRNA to knockdown XIST to evaluate cell growth and apoptosis in U2OS cells in vitro and xenograft formation in vivo. An observed relationship between XIST and the p53 upregulated modulator of apoptosis (PUMA) and nuclear factor-kappa B (NF-kB) pathway was further explored by using small interfering RNA (siRNA). Our results showed that suppression of XIST by short hairpin RNA (shRNA) impeded U2OS cell growth, induced apoptosis and lessened OS xenograft tumor growth. Targeting XIST increased NF-kB-dependent PUMA upregulation in U2OS cells. Upregulation of PUMA is correlated with suppression of XIST-induced apoptosis in U2OS cells. Therefore, inhibition of XIST could promote U2OS cell death via activation of NF-kB/PUMA pathways.

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Long noncoding RNA XIST participates in bladder cancer by downregulating p53 via binding to TET1

Cited by 31 publications

References 32 publications

Hydroxyurea promotes TET1 expression and induces apoptosis in osteosarcoma cells

Hydroxyurea promotes TET1 expression and induces apoptosis in osteosarcoma cells

Long noncoding RNAs, ENST00000598996 and ENST00000524265, are correlated with favorable prognosis and act as potential tumor suppressors in bladder cancer

Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal

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