Long noncoding RNAs: new insights in modulating mammalian spermatogenesis

Sahlu, Bahlibi Weldegebriall; Zhao, Shanjiang; Wang, Xiuge; Umer, Saqib; Zou, Huiying; Huang, Jinming; Zhu, Huabin

doi:10.1186/s40104-019-0424-8

Cited by 28 publications

(24 citation statements)

References 93 publications

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“…Moreover, a majority of these transcripts do not get translated into proteins. These transcripts which are not translated into proteins are known as the noncoding RNAs (ncRNAs) [ 1 , 2 ]. Earlier, ncRNAs were considered as ‘junk’, but we realized the importance of this junk chunk only recently [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility

Joshi

Rajender

2020

Reprod Biol Endocrinol

100

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Background Long non-coding RNAs (lncRNAs) have a size of more than 200 bp and are known to regulate a host of crucial cellular processes like proliferation, differentiation and apoptosis by regulating gene expression. While small noncoding RNAs (ncRNAs) such as miRNAs, siRNAs, Piwi-interacting RNAs have been extensively studied in male germ cell development, the role of lncRNAs in spermatogenesis remains largely unknown. Objective In this article, we have reviewed the biology and role of lncRNAs in spermatogenesis along with the tools available for data analysis. Results and conclusions Till date, three microarray and four RNA-seq studies have been undertaken to identify lncRNAs in mouse testes or germ cells. These studies were done on pre-natal, post-natal, adult testis, and different germ cells to identify lncRNAs regulating spermatogenesis. In case of humans, five RNA-seq studies on different germ cell populations, including two on sperm, were undertaken. We compared three studies on human germ cells to identify common lncRNAs and found 15 lncRNAs (LINC00635, LINC00521, LINC00174, LINC00654, LINC00710, LINC00226, LINC00326, LINC00494, LINC00535, LINC00616, LINC00662, LINC00668, LINC00467, LINC00608, and LINC00658) to show consistent differential expression across these studies. Some of the targets of these lncRNAs included CENPB, FAM98B, GOLGA6 family, RPGR, TPM2, GNB5, KCNQ10T1, TAZ, LIN28A, CDKN2B, CDKN2A, CDKN1A, CDKN1B, CDKN1C, EZH2, SUZ12, VEGFA genes. A lone study on human male infertility identified 9879 differentially expressed lncRNAs with three (lnc32058, lnc09522, and lnc98497) of them showing specific and high expression in immotile sperm in comparison to normal motile sperm. A few lncRNAs (Mrhl, Drm, Spga-lncRNAs, NLC1-C, HongrES2, Tsx, LncRNA-tcam1, Tug1, Tesra, AK015322, Gm2044, and LncRNA033862) have been functionally validated for their roles in spermatogenesis. Apart from rodents and humans, studies on sheep and bull have also identified lncRNAs potentially important for spermatogenesis. A number of these non-coding RNAs are strong candidates for further research on their roles in spermatogenesis.

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Section: Introductionmentioning

confidence: 99%

“…Many potential lncRNAs have been identified in male germ cell development, some of them showing differential expression between fertile and infertile sperm. However, only a few have been functionally annotated and characterized [ 1 ] (Fig. 4 ).…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility

Joshi

Rajender

2020

Reprod Biol Endocrinol

100

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“…Recent research has focused increasingly on lncRNAs, which control many genetic pathways. 24,28,29 Studies from the last decade have increasingly highlighted the important roles of lncRNAs in GBM. 30,32 Dysregulated lncRNAs can modulate the malignant processes associated with GBM initiation and progression.…”

Section: Discussionmentioning

confidence: 99%

<p>Long Intergenic Non-Protein Coding RNA 1094 Promotes Initiation and Progression of Glioblastoma by Promoting microRNA-577-Regulated Stabilization of Brain-Derived Neurotrophic Factor</p>

Dong¹,

Fu²,

Yu³

et al. 2020

CMAR

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Purpose: The long intergenic non-protein coding RNA 1094 (LINC01094) plays a vital role in the oncogenicity of clear cell renal cell carcinoma. However, its expression profile and detailed roles in glioblastoma (GBM) remain unknown. In this study, we mainly investigated the expression and roles of LINC01094 in GBM and focused on the mechanism by which LINC01094 regulates the malignant characteristics of GBM. Patients and Methods: LINC01094 expression in GBM was determined with quantitative reverse transcription polymerase chain reaction. The proliferation, apoptosis, migration, invasion in vitro, and tumor growth in vivo of GBM cells were evaluated using Cell Counting Kit-8 assay, flow cytometry analysis, migration assay, invasion assay, and tumor xenograft models, respectively. Results: LINC01094 was overexpressed in GBM tissues and cell lines. Moreover, increased LINC01094 expression was associated with adverse clinicopathological parameters in patients with GBM. Loss of LINC01094 inhibited GBM cell proliferation, migration, and invasion; promoted cell apoptosis; and suppressed tumor growth in vivo. Mechanically, LINC01094 functioned as a molecular sponge for microRNA-577 (miR-577) and consequently enhanced the expression of brain-derived neurotrophic factor (BDNF) in GBM cells. Both miR-577 inhibition and BDNF expression enhancement reversed LINC01094 deficiency-mediated inhibition of malignant processes in GBM cells. Conclusion: Our results verified the involvement of the LINC01094/miR-577/BDNF pathway in GBM cells and its enhancing effects on the aggressive behaviors of GBM cells in vitro and in vivo. This pathway may be a novel and promising focus for the future development of targeted therapies for GBM.

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“…Spermatozoa comprise a wide repertoire of RNAs that are implicated in several biological processes associated with spermatogenesis, fertilization and embryo development [ 1 , 2 , 3 , 4 , 5 ]. In eukaryotes, the vast majority of gene transcripts are non-coding RNAs (ncRNAs) [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…While once thought to be “junk” in the sequences of genomes, accumulating evidence has shown that ncRNAs are implicated in numerous important biological processes, such as transcription, splicing, translation, epigenetic modifications, cell development and differentiation [ 3 , 5 , 6 , 7 , 8 ]. Recently, several reports have focused on the biological functions of long non-coding RNAs (lncRNAs) [ 4 , 6 , 8 ]. Evidence has shown that the lncRNAs represent the largest part of the transcriptome in mammals, but only a small proportion has been functionally characterized [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Regulatory Potential of Long Non-Coding RNAs (lncRNAs) in Boar Spermatozoa with Good and Poor Freezability

Fraser

Paukszto

Mańkowska

et al. 2020

Life

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Long non-coding RNAs (lncRNAs) are suggested to play an important role in the sperm biological processes. We performed de novo transcriptome assembly to characterize lncRNAs in spermatozoa, and to investigate the role of the potential target genes of the differentially expressed lncRNAs (DElncRNAs) in sperm freezability. We detected approximately 4007 DElncRNAs, which were differentially expressed in spermatozoa from boars classified as having good and poor semen freezability (GSF and PSF, respectively). Most of the DElncRNAs were upregulated in boars of the PSF group and appeared to significantly affect the sperm’s response to the cryopreservation conditions. Furthermore, we predicted that the potential target genes were regulated by DElncRNAs in cis or trans. It was found that DElncRNAs of both freezability groups had potential cis- and trans-regulatory effects on different protein-coding genes, such as COX7A2L, TXNDC8 and SOX-7. Gene Ontology (GO) enrichment revealed that the DElncRNA target genes are associated with numerous biological processes, including signal transduction, response to stress, cell death (apoptosis), motility and embryo development. Significant differences in the de novo assembled transcriptome expression profiles of the DElncRNAs between the freezability groups were confirmed by quantitative real-time PCR analysis. This study reveals the potential effects of protein-coding genes of DElncRNAs on sperm functions, which could contribute to further research on their relevance in semen freezability.

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Long noncoding RNAs: new insights in modulating mammalian spermatogenesis

Cited by 28 publications

References 93 publications

Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility

Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility

<p>Long Intergenic Non-Protein Coding RNA 1094 Promotes Initiation and Progression of Glioblastoma by Promoting microRNA-577-Regulated Stabilization of Brain-Derived Neurotrophic Factor</p>

Regulatory Potential of Long Non-Coding RNAs (lncRNAs) in Boar Spermatozoa with Good and Poor Freezability

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