Long-term consequences of bilateral cavernous crush injury in normal and diabetic rats: a functional study

Castiglione, Fabio; Albersen, Maarten; Fiorenzo, Salvatore; Hedlund, Petter; Çakır, Ömer Onur; Pavone, Carlo; Alnajjar, Hussain M.; Joniau, Steven; Muneer, Asıf; Kluth, Luis; Campos-Juanatey, Félix; Cocci, Andrea; Rosenbaum, Clemens M.; Vetterlein, Malte W.; Ascanio, Enrique Fes; Adamowicz, Jan

doi:10.1038/s41443-021-00474-z

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…A recent study by Castiglione et al [ 33 ] investigated the long-term effects of BNCI rats with type 1 diabetes and found that, unlike healthy rats, diabetic rats did not show natural recovery of erectile function at 4 months after BNCI. Zhang et al [ 28 ] suggested that stem cell injection is effective in treating diabetes-induced ED in a rat model.…”

Section: Discussionmentioning

confidence: 99%

Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model

Moon

Kim

et al. 2023

Bioengineering

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Erectile dysfunction (ED) is a common and feared complication of radical prostatectomy (RP) for prostate cancer. Recently, tissue engineering for post-prostatectomy ED has been attempted in which controlled interactions between cells, growth factors, and the extracellular matrix (ECM) are important for the structural integrity if nerve regeneration. In this study, we evaluated the effects of a biomechanical ECM patch on the morphology and behavior of human bone marrow-derived mesenchymal stem cells (hBMSCs) in a bilateral cavernous nerve injury (BCNI) rat model. The ECM patch, made of decellularized human fibroblast-derived ECM (hFDM) and a biocompatible polyvinyl alcohol (PVA) hydrogel, was tested with human bone marrow-derived mesenchymal stem cells (hBMSCs) on a bilateral cavernous nerve injury (BCNI) rat model. In vitro analysis showed that the hFDM/PVA + hBMSCs patches significantly increased neural development markers. In vivo experiments demonstrated that the rats treated with the hFDM/PVA patch had higher ICP/MAP ratios, higher ratios of smooth muscle to collagen, increased nNOS content, higher levels of eNOS protein expression, and higher cGMP levels compared to the BCNI group. These results indicate that the hFDM/PVA patch is effective in promoting angiogenesis, smooth muscle regeneration, and nitrergic nerve regeneration, which could contribute to improved erectile function in post-prostatectomy ED.

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Section: Discussionmentioning

confidence: 99%

Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model

Moon

Kim

et al. 2023

Bioengineering

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The Current Role and Implications of Stem Cell Therapy in Erectile Dysfunction: A Transformation from Caterpillar to Butterfly Is Required

Castiglione

Çakır

Satchi

et al. 2023

European Urology Focus

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An animal model induced by bilateral cavernous nerve crushing mimics post-radical prostatectomy erectile dysfunction in old rats

et al. 2023

Life Sciences

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Long-term consequences of bilateral cavernous crush injury in normal and diabetic rats: a functional study

Cited by 3 publications

References 48 publications

Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model

Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model

The Current Role and Implications of Stem Cell Therapy in Erectile Dysfunction: A Transformation from Caterpillar to Butterfly Is Required

An animal model induced by bilateral cavernous nerve crushing mimics post-radical prostatectomy erectile dysfunction in old rats

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