Long-term effects and parental RNAi in the blood feeder Rhodnius prolixus (Hemiptera; Reduviidae)

Paim, Rafaela M.M.; Araújo, Ricardo Nascimento; Lehane, Michael J.; Gontijo, Nelder F.; Pereira, Marcos H.

doi:10.1016/j.ibmb.2013.08.008

Cited by 46 publications

(40 citation statements)

References 28 publications

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“…Parental or transgenerational RNAi effects have been observed in both coleopteran (Bucher et al, 2002;Khajuria et al, 2015;Shukla and Palli, 2014) and hemipteran insects (Coleman et al, 2015;Liu and Kaufman, 2004;Mao et al, 2013;Paim et al, 2013). For example, Liu and Kaufman (2004) used pRNAi via injection of dsRNA into the adult female to investigate the developmental role of the hunchback gene in the milkweed bug, Oncopeltus fasciatus (Hemiptera: Lygaeidae).…”

Section: Introductionmentioning

confidence: 99%

Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)

Fishilevich¹,

Vélez

Khajuria

et al. 2016

Insect Biochemistry and Molecular Biology

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Section: Introductionmentioning

confidence: 99%

Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)

Fishilevich¹,

Vélez

Khajuria

et al. 2016

Insect Biochemistry and Molecular Biology

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“…In the brown planthopper, N. lugens , distal‐less dsRNA injections in fifth instar nymphs caused alteration of claws in less than 5% of the progeny (Xu et al, 2013). In the kissing bug, Rhodnius prolixus , on the other hand, giant dsRNA injections in adult virgin females affected 97.4% of the embryos (Lavore et al., ), and nitrophorins 1–4 and rhodniin dsRNA injections in fifth instar nymphs induced the respective phenotypes in more than 70% of the progeny (Paim et al., ). Thus, parental RNAi has been used to elucidate the functions of genes that are essential for embryogenesis.…”

Section: Discussionmentioning

confidence: 99%

GENE SILENCING BY PARENTAL RNA INTERFERENCE IN THE GREEN RICE LEAFHOPPER, Nephotettix cincticeps (HEMIPTERA: CICADELLIDAE)

Matsumoto

Hattori

2016

Arch Insect Biochem Physiol

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RNA interference (RNAi) has been widely used for investigating gene function in many nonmodel insect species. Parental RNAi causes gene knockdown in the next generation through the administration of double-strand RNA (dsRNA) to the mother generation. In this study, we demonstrate that parental RNAi mediated gene silencing is effective in determining the gene function of the cuticle and the salivary glands in green rice leafhopper (GRH), Nephotettix cincticeps (Uhler). Injection of dsRNA of NcLac2 (9 ng/female) to female parents caused a strong knockdown of laccase-2 gene of first instar nymphs, which eventually led to high mortality rates and depigmentation of side lines on the body. The effects of parental RNAi on the mortality of the nymphs were maintained through 12-14 days after the injections. We also confirmed the effectiveness of parental RNAi induced silencing on the gene expressed in the salivary gland, the gene product of which is passed from instar to instar. The parental RNAi method can be used to examine gene function by phenotyping many offspring nymphs with injection of dsRNA into a small number of parent females, and may be applicable to high-efficiency determination of gene functions in this species.

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“…Rhodnius has a genome size of 733 Mbp and a short life cycle (around 4 months) among the Triatominae (Gomes et al ., ; Mesquita et al ., ; Panzera et al ., ), Figure ). Gene function can be addressed by RNA interference (RNAi): the injection of dsRNA molecules in adults produces a systemic and long‐lasting downregulation of the cognate gene (Paim et al ., ). Likewise, bacterial strains engineered to express dsRNA moieties against Rhodnius genes can also induce efficient knockdown when fed to adult insects (Durvasula et al ., ; Matthews et al ., ).…”

Section: Historical Considerationsmentioning

confidence: 97%

“…the injection of dsRNA molecules in adults produces a systemic and long-lasting downregulation of the cognate gene (Paim et al, 2013).…”

Section: H I S T O R I C a L C O N S I D E R A T I O N Smentioning

confidence: 99%

Rhodnius prolixus: From classical physiology to modern developmental biology

Fonseca

Berni

Tobias-Santos

et al. 2017

Genesis

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The hemiptera Rhodnius prolixus is a blood-feeding insect and a primary vector of Trypanosoma cruzi, the etiological agent of the Chagas disease. Over the past century, Rhodnius has been the subject of intense investigations, which have contributed to unveil important aspects of metabolism and physiology in insects. Recent technological innovations are helping dissect the genetic and molecular underpinnings of Rhodnius embryogenesis and organogenesis, thus fostering the use of this important species in the fields of developmental and evolutionary biology. Rhodnius represents also an excellent system to study development under stressful conditions, since the embryo must develop in the presence of a large amount of blood-derived reactive oxygen species. With a recently sequenced genome, small among other Hemiptera, and the identification of basic elements for all classical development pathways, functional studies in this species are revealing novel aspects of insect development and evolution. Here we review early studies on this model insect and how this paved the way for recent functional studies using the kissing bug.

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Long-term effects and parental RNAi in the blood feeder Rhodnius prolixus (Hemiptera; Reduviidae)

Cited by 46 publications

References 28 publications

Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)

Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)

GENE SILENCING BY PARENTAL RNA INTERFERENCE IN THE GREEN RICE LEAFHOPPER, Nephotettix cincticeps (HEMIPTERA: CICADELLIDAE)

Rhodnius prolixus: From classical physiology to modern developmental biology

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