The challenges posed by the various factors that affect the loss of surface and underground aquifers worldwide, specifically in Mexico, are increasingly severe. This has forced scientists and technicians to look for alternatives to conserve, sustain, and ensure the sustainability of liquid deposits, whether surface or underground in nature. Based on the above, it was proposed to evaluate the technical and technological feasibility of constructing a well for the artificial injection of water into the subsoil in the Los Elizondo Gallery in the Huajuco area. The objective was to provide a technical and technological guide for the planning, design, and development of groundwater conservation by deep injection. The methodology corresponded to a feasible project, in which the procedures for the construction of the artificial water injection well to underground causes were followed. The technical and technological feasibility of the construction of the well was evaluated, including the examination of the geological and hydrological conditions of the area, as well as the available technologies and materials that can be used to build the well. The execution of the project led to the determination that the construction of a well for the artificial injection of water into the subsoil is technically and technologically feasible in the area of the Los Elizondo Gallery. Once the appropriate procedures were applied, the Guide for aquifer recharge through deep injection wells in karst environments was developed: a case study Los Elizondo Gallery, Mexico.