Long-term exposure to low doses of aluminum affects mineral content and microarchitecture of rats alveolar bone

Souza-Monteiro, Deiweson; Ferreira, Railson de Oliveira; Eiró, Luciana Guimarães; Lima, Leidiane Alencar de Oliveira; Balbinot, Gabriela de Souza; Paz, Simone Patrícia Aranha da; Albuquerque, Alan Rodrigo Leal; Collares, Fabrício Mezzomo; Angélica, Rômulo Simões; Pessanha, Sofia; Maia, Cristiane do Socorro Ferraz; Lima, Rafael Rodrigues

doi:10.1007/s11356-021-13937-z

Cited by 14 publications

(5 citation statements)

References 66 publications

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“…Based on this, the threshold was set from 120 to 255. Trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), and bone volume to tissue volume ratio (BV/TV) were measured with the BoneJ plugin [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

Açaí (Euterpe oleracea Mart.) Attenuates Oxidative Stress and Alveolar Bone Damage in Experimental Periodontitis in Rats

et al. 2022

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Açaí (Euterpe oleracea Mart.) juice is rich in phenolic compounds with high antioxidant capacity. It has been observed that the use of antioxidants may be an additional strategy to nonsurgical periodontal therapy as well as to prevent alveolar bone loss. Thus, the objective of this study was to investigate the effects of açaí supplementation on experimental periodontitis in rats. Twenty male Rattus norvegicus (Wistar) rats were assigned into control, açaí, experimental periodontitis, and experimental periodontitis with açaí supplementation groups. Periodontitis was induced by placing ligatures around the lower first molars. Animals in the açaí groups received 0.01 mL/g of clarified açaí juice for 14 days by intragastric gavage. At the end of the experimental period, blood was collected to assess the reduced glutathione (GSH), Trolox equivalent antioxidant capacity (TEAC), and lipid peroxidation (TBARS) levels. Moreover, hemimandibles were analyzed by micro-computed tomography (micro-CT) for alveolar bone loss and bone quality. Açaí supplementation increased blood total antioxidant capacity and decreased lipid peroxidation. It also reduced alveolar bone loss when compared to the experimental periodontitis group. Moreover, clarified açaí per se modulated the oxidative biochemistry and bone microstructure. Thus, açaí may be considered a viable alternative for managing periodontal oxidative stress and preventing alveolar bone loss.

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Section: Methodsmentioning

confidence: 99%

Açaí (Euterpe oleracea Mart.) Attenuates Oxidative Stress and Alveolar Bone Damage in Experimental Periodontitis in Rats

et al. 2022

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“…The dose used in the present study was established and described in previous studies by our group, corresponding to 1.5 mg/kg/day of the accepted dose for the human diet [ 16 , 29 , 81 , 82 ]. Allometric calculation conversion was necessary, considering the biological differences between humans and rodents [ 82 , 83 ].…”

Section: Methodsmentioning

confidence: 99%

Global Proteomic Profile of Aluminum-Induced Hippocampal Impairments in Rats: Are Low Doses of Aluminum Really Safe?

Bittencourt

Silva

Aragão

et al. 2022

IJMS

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Hippocampus is the brain area where aluminum (Al) accumulates in abundance and is widely associated with learning and memory. In the present study, we evaluate behavioral, tissue, and proteomic changes in the hippocampus of Wistar rats caused by exposure to doses that mimic human consumption of aluminum chloride (AlCl3) in urban areas. For this, male Wistar rats were divided into two groups: Control (distilled water) and AlCl3 (8.3 mg/kg/day), both groups were exposed orally for 60 days. After the Al exposure protocol, cognitive functions were assessed by the Water maze test, followed by a collection for analysis of the global proteomic profile of the hippocampus by mass spectrometry. Aside from proteomic analysis, we performed a histological analysis of the hippocampus, to the determination of cell body density by cresyl violet staining in Cornu Ammonis fields (CA) 1 and 3, and hilus regions. Our results indicated that exposure to low doses of aluminum chloride triggered a decreased cognitive performance in learning and memory, being associated with the deregulation of proteins expression, mainly those related to the regulation of the cytoskeleton, cellular metabolism, mitochondrial activity, redox regulation, nervous system regulation, and synaptic signaling, reduced cell body density in CA1, CA3, and hilus.

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“…To ensure Al consumption, AlCl 3 was administered by orogastric gavage. In previous studies, this method triggered harmful effects in the central nervous system [10] and in other oral structures such as alveolar bone [26], and the success of this method was validated by the quantification of the blood concentration of Al. In those cited studies, the animals exposed to AlCl 3 exhibited higher levels of Al in the blood in comparison with the non-exposed animals, without significant alterations in weight and mortality between the groups.…”

Section: Discussionmentioning

confidence: 98%

Salivary Glands after Prolonged Aluminum Exposure: Proteomic Approach Underlying Biochemical and Morphological Impairments in Rats

Souza-Monteiro

Bittencourt

et al. 2022

IJMS

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Aluminum (Al) is one of the most abundant elements on Earth, and its high extraction rate and industrial use make human exposure very common. As Al may be a human toxicant, it is important to investigate the effects of Al exposure, mainly at low doses and for prolonged periods, by simulating human exposure. This work aimed to study the effects of low-dose exposure to chloride aluminum (AlCl3) on the oxidative biochemistry, proteomic profile, and morphology of the major salivary glands. Wistar male rats were exposed to 8.3 mg/kg/day of AlCl3 via intragastric gavage for 60 days. Then, the parotid and submandibular glands were subjected to biochemical assays, proteomic evaluation, and histological analysis. Al caused oxidative imbalance in both salivary glands. Dysregulation of protein expression, mainly of those related to cytoarchitecture, energy metabolism and glandular function, was detected in both salivary glands. Al also promoted histological alterations, such as acinar atrophy and an increase in parenchymal tissue. Prolonged exposure to Al, even at low doses, was able to modulate molecular alterations associated with morphological impairments in the salivary glands of rats. From this perspective, prolonged Al exposure may be a risk to exposed populations and their oral health.

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Long-term exposure to low doses of aluminum affects mineral content and microarchitecture of rats alveolar bone

Cited by 14 publications

References 66 publications

Açaí (Euterpe oleracea Mart.) Attenuates Oxidative Stress and Alveolar Bone Damage in Experimental Periodontitis in Rats

Açaí (Euterpe oleracea Mart.) Attenuates Oxidative Stress and Alveolar Bone Damage in Experimental Periodontitis in Rats

Global Proteomic Profile of Aluminum-Induced Hippocampal Impairments in Rats: Are Low Doses of Aluminum Really Safe?

Salivary Glands after Prolonged Aluminum Exposure: Proteomic Approach Underlying Biochemical and Morphological Impairments in Rats

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