Long term fermentation studies about the nutritional requirements for biogasification of fodder beet silage as mono-substrate

Scherer, Paul; Neumann, L.; Demirel, Burak; Schmidt, Olaf; Unbehauen, M.

doi:10.1016/j.biombioe.2009.01.011

Cited by 41 publications

(33 citation statements)

References 21 publications

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“…Our results seem to contradict these assumptions, as they clearly demonstrate that hydrogenotrophic methanogens can dominate during a thermophilic fermentation process with renewable biomass (16,(49)(50)(51)). It appears that temperature has a decisive influence on the type of archaeal morphotypes present, as rod-like methanogens dominated at 60°C periods, whereas different morphotypes of methanogens appeared when 55°C conditions were enabled.…”

mentioning

confidence: 58%

“…This work focused on the methanogens which directly reduce CO 2 to CH 4 or use acetate or methylated C 1 compounds as the main substrate to yield methane (35). However, about 65 to 70% of methane produced by methanogens is assumed to originate from acetate (4, 5), and the so-called acetoclastic Euryarchaeota are also dominant in many biogas fermentors used for anaerobic wastewater treatment and sewage sludge digestion (17,24,30,53).Our results seem to contradict these assumptions, as they clearly demonstrate that hydrogenotrophic methanogens can dominate during a thermophilic fermentation process with renewable biomass (16,(49)(50)(51)). It appears that temperature has a decisive influence on the type of archaeal morphotypes present, as rod-like methanogens dominated at 60°C periods, whereas different morphotypes of methanogens appeared when 55°C conditions were enabled.…”

mentioning

confidence: 74%

“…The supernatant was mixed 1:9 with 1,4-diazabicyclo[2.2.2]octane (DABCO; antifading reagent) (25 mg/ml) and examined microscopically on a slide coated with 2.5% methylcellulose. Bacterial cells were generally examined using phase-contrast microscopy, while cells of methanogens were specifically detected by fluorescence excitation at 420 nm (emission wavelength, 490 nm) at a magnification of ϫ640 (49). Five phasecontrast and fluorescence images of each sample were captured with a digital Leica DC300 microscope camera and evaluated using image analysis software (Image Pro Plus 6.0; Media Cybernetics, Bethesda, MD).…”

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confidence: 99%

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Anaerobic Digestion of Renewable Biomass: Thermophilic Temperature Governs Methanogen Population Dynamics

Krakat

Westphal

Schmidt

et al. 2010

Appl Environ Microbiol

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113

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Beet silage and beet juice were digested continuously as representative energy crops in a thermophilic biogas fermentor for more than 7 years. Fluorescence microscopy of 15 samples covering a period of 650 days revealed that a decrease in temperature from 60°C to 55°C converted a morphologically uniform archaeal population (rods) into a population of methanogens exhibiting different cellular morphologies (rods and coccoid cells). A subsequent temperature increase back to 60°C reestablished the uniform morphology of methanogens observed in the previous 60°C period. In order to verify these observations, representative samples were investigated by amplified rRNA gene restriction analysis (ARDRA) and fluorescence in situ hybridization (FISH). Both methods confirmed the temperature-dependent population shift observed by fluorescence microscopy. Moreover, all samples investigated demonstrated that hydrogenotrophic Methanobacteriales dominated in the fermentor, as 29 of 34 identified operational taxonomic units (OTUs) were assigned to this order. This apparent discrimination of acetoclastic methanogens contradicts common models for anaerobic digestion processes, such as anaerobic digestion model 1 (ADM1), which describes the acetotrophic Euryarchaeota as predominant organisms.The replacement of fossil fuels by renewable energy sources such as agricultural crops is gaining momentum internationally as a means to decrease emissions from conventional fuel sources impacting global warming (39). Thereby, biogasification using energy crops is the only fuel-producing process with a closed CO 2 and nutrient cycle (8). The production of biogas from plant waste or other organic materials is a feasible strategy in view of both ecology and economy (63). Fodder beet was chosen as the renewable biomass source for a thermophilic biogas fermentor because the European Union decreased the regulatory price for sugar beets in 2006, and therefore many farmers are looking for an alternative use. Furthermore, fodder beet was considered an attractive renewable energy crop due to its high methane yield per hectare (67), as well as the ideal ensiling conditions enabling the storage of beet silage for many years. Furthermore, the sugar beet was only recently identified as one of the most sustainable energy crops with regard to its water footprint when used for biofuel production (22).A long-term experiment was started on 4 July 2001 (see reference 48 for startup details), and the same biogas fermentors are still running stable due to the use of fuzzy logic control (16,48). During the conversion of biomass to methane, four different microbial processes can be distinguished: hydrolysis, acidogenesis, acetogenesis, and methanogenesis (17, 69). Population changes might therefore impact the entire community by triggering an imbalance that is reflected in the bioreactor performance via accumulation of intermediates such as volatile fatty acids (mainly C 2 and C 3 ), via pH changes, or via reduced efficiency (52). This work focused on the methanogens w...

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confidence: 58%

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confidence: 74%

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confidence: 99%

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Anaerobic Digestion of Renewable Biomass: Thermophilic Temperature Governs Methanogen Population Dynamics

Krakat

Westphal

Schmidt

et al. 2010

Appl Environ Microbiol

Self Cite

113

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“…Besides the traditional use of fodder beet as the dietary food new ways of use appear. It is the use in bioenergetics, such as the production of bioethanol (Reed et al, 1986;Mähnert and Linke, 2009;Chochola, 2007;Pulkrábek et al 2007) and biogas (Scherer, et al, 2009;Klocke et al, 2007). Use of fodder beet as a source of renewable energy results from the fact that it provides more energy than cereals and fodder crops Hnilička et al, 2009;Martínez-Pérez et al, 2007).…”

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confidence: 99%

Technologies and Varieties of Fodder Beet in Organic Farming

Honsová¹,

Hnilička²,

Bečka³

et al. 2011

Research in Organic Farming

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“…It has been reported that anaerobic digestion of lignocellulosic biomasses alone for biogas production is prone to instability and process failure (Scherer et al 2009;Weiland, 2010). In some literature (Cuetos et al 2011;Li et al 2014), co-digestion of agricultural residues with manure has been reported to greatly improve the anaerobic digestion of agricultural residues due to the various nutrients in manure.…”

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confidence: 99%

Comparison of Reactor Configurations for Biogas Production from Rapeseed Straw

Yuan

2016

BioResources

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To investigate the effects of reactor configurations on the anaerobic digestion performance of agricultural residue rapeseed straw, semicontinuous, one-stage, continuously stirred tank reactors (CSTR) and batch, two-stage, leach bed-upflow anaerobic sludge blanket (UASB) reactors were operated at the same hydraulic retention time (HRT) (30 days) and target organic loading rate (OLR) (3.0 gVS/L.d). In the continuously loaded CSTR, the specific methane yields did not substantially change as the OLR increased from 1.5 gVS/L.d to 3.0 gVS/L.d during the four periods. Conversely, the specific methane yields in the batch-fed leach bed-UASB increased considerably with the increase in OLR. The leach bed reactor contributed 75% of the total yield, while the UASB reactor contributed only 25% of the total yield. The total specific methane yields were 108 mL/gVS and 160 mL/gVS for the leach bed-UASB and CSTR, respectively, while the volatile solid (VS) reductions of the rapeseed straw were 27.1% and 36.6%, respectively. The results indicated that the process performance was more efficient in the CSTR than in the leach bed-UASB for the digestion of rapeseed straw. Biogas production was clearly affected by the reactor configurations.

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Long term fermentation studies about the nutritional requirements for biogasification of fodder beet silage as mono-substrate

Cited by 41 publications

References 21 publications

Anaerobic Digestion of Renewable Biomass: Thermophilic Temperature Governs Methanogen Population Dynamics

Anaerobic Digestion of Renewable Biomass: Thermophilic Temperature Governs Methanogen Population Dynamics

Technologies and Varieties of Fodder Beet in Organic Farming

Comparison of Reactor Configurations for Biogas Production from Rapeseed Straw

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