Long-Term Fertilization Alters Mycorrhizal Colonization Strategy in the Roots of Agrostis capillaris

Corcoz, Larisa; Păcurar, F.; Pop-Moldovan, Victoria; Vaida, Ioana; Pleşa, Anca; Stoian, Vlad; Vidican, Roxana

doi:10.3390/agriculture12060847

Cited by 4 publications

(5 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Arbuscular mycorrhizal (AM) fungi colonization is a weak indicator of the decline or decay presence in a forest. The presence/absence of arbuscules and vesicles, combined with the intensity of colonization, was used to determine the colonization strategy [36,39]. This approach enables the detection of resistance colonization (at intensities lower than 10%), proliferative strategies (intensities between 10 and 25%, but lack of arbuscules or vesicles), respectively, transfer strategies (intensities higher than 25% and arbuscules present), and storage strategies (intensities higher than 25% and vesicles present).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Soil Properties and Forest Decline in the North-Western Part of Romania

Onet,

Vidican,

Ghergheles

et al. 2024

Forests

Self Cite

View full text Add to dashboard Cite

The paper presents the study of the soil quality and health expressed by the chemical and biological properties in a research field placed at Varciorog, Bihor County, Romania. The soil samples were collected from 3 soil variants in March 2023. In each soil variant, some soil chemical parameters and the abundance of bacteria were determined. The frequency and intensity of colonization, along with arbuscules and vesicles, were scored to determine the mycorrhizal potential of each soil. The community-level physiological profile was used to determine the functional microbiome and its ability to decompose a specific set of substrates. In the control variant (CTRL), which is a functional forest cultivated with beech in a proportion of 90%, the soil properties were compared with those determined from Site 1 (a declined mixed forest) and from Site 2 (chestnut forest in a stage of complete drying). The data were statistically processed with a one-way ANOVA test, followed by the Duncan post-hoc test, which revealed significant variation in the potential of microbial functional communities across the analyzed sites. Also, the soil parameters that significantly varied in the 3 soil variants were bacterial number, pH, humus, exchangeable aluminum, coarse sand, dust, and fine sand. The Pearson correlation was computed to study the links between bacterial numbers and chemical parameters. The results showed strong correlations between most of the studied soil properties. The Ecoplates approach to soil functional microbiome highlighted various differences between the microbial communities of the three tested sites. Mycorrhizal colonization shows different potentials for symbiosis formation. The peak of mycorrhizal colonization was in declined forest, with 43.36% colonization frequency and 24.56% intensity. Arbuscules reached 11.36% in declined forest, while in control and decayed sites, the indicator was under 4%. Vesicles are more associated with control and decayed forests, with values of presence over 1.30%. As an indicator of microbial general activity, the sum of recorded activities was higher in declined and decayed forests. At these sites, the activity of the functional microbiome was amplified. The decline process activates a higher diversity of functional groups and is associated with a larger area of substrate decomposition capacity, which indicates a more extensive range of microbial functions related to breaking down organic matter.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The arbuscular mycorrhizal potential was calculated to identify the native symbiotic potential of each soil [34][35][36]. Trifolium repens was chosen as a mycorrhizal tester for its adaptability and rapid growth in any type of soil condition.…”

Section: Determination Of Arbuscular Mycorrhizal Potentialmentioning

confidence: 99%

Soil Properties and Forest Decline in the North-Western Part of Romania

Onet,

Vidican,

Ghergheles

et al. 2024

Forests

Self Cite

View full text Add to dashboard Cite

show abstract

“…Each set of data from treated and untreated variants was filtered and grouped based on intensity, arbuscules, and vesicles, in order to identify the number of clear colonization strategies ( Corcoz et al., 2022b ). Variants with an intensity lower than 10% were considered in the group of resistance colonization conditions; the intensity between 10% and 25% was assessed as proliferative colonization strategy; the roots with an intensity higher than 25% and an arbuscule/vesicle ratio higher than 1.0 were considered in the group of transfer colonization strategy; the roots with an intensity higher than 25% and an arbuscule/vesicle ratio lower than 1.0 were considered in the group of storage colonization strategy ( Corcoz et al., 2022a ). The strategy graph was performed with the package “scatterplot3d” ( Ligges and Mächler, 2003 ).…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, the maps with the maximum arbuscules and vesicles from each treatment × phenophase combination were extracted to analyze the mycorrhizal expansion and developed structures in the roots. All maps were analyzed with an expanded version of the multi-point analysis method ( Corcoz et al., 2022a ), which permits the simultaneous comparison of different colonized areas from multiple maps. For each mycorrhizal parameter, starting with the second growth stage, the values of all parameters from the previous growth stages were used to create a forecast model.…”

Section: Methodsmentioning

confidence: 99%

Models of mycorrhizal colonization patterns and strategies induced by biostimulator treatments in Zea mays roots

et al. 2022

Self Cite

View full text Add to dashboard Cite

Agronomic inputs and technologies, especially fertilizers, act on the evolution of the symbiotic partnership between arbuscular mycorrhizal fungi and cultivated plants. The use of the MycoPatt method for the assessment of mycorrhizas in maize roots leads to the extraction of large parameter databases with an increased resolution over the colonization mechanism. The application of a biostimulator treatment on plants acted toward a reduction of root permissiveness for mycorrhizas. The phenomenon was noticeable through an increased colonization variability that overlapped with plant nutritional needs. The annual characteristic of the plant was highlighted by the simultaneous presence of arbuscules and vesicles, with a high share of arbuscules in the advanced phenophases. Colonized root parts presented numerous arbuscule-dominated areas in all phenophases, which indicated a continuous formation of these structures and an intense nutrient transfer between partners. Mycorrhizal maps showed the slowing effect of the biostimulators on colonization, with one phenophase delay in the case of biostimulated plants compared to the ones without biostimulators. The forecast models presented gradual colonization in plants without biostimulators, with the expansion of new hyphal networks. The use of biostimulators on plants exhibited a lower permissiveness for new colonization areas, and the mechanism relies on hyphae developed in the former phenophases.

show abstract

“…Unfortunately, intensive fertilization and tillage damage AMF diversity, infectiveness, and effectiveness. The developmental strategy of AMF symbiosis can be represented on a map by converting the colonization types of plots to colors [9]. It was demonstrated by investigating the effect of long-term organic and mineral fertilization on AMF root colonization parameters and the colonization pattern of Agrostis capillaris estimated by MycoPatt tool was translated into colors.…”

mentioning

confidence: 99%