Long term intravital single cell tracking under multiphoton microscopy

Liang, Yajie; Walczak, Piotr

doi:10.1016/j.jneumeth.2020.109042

Cited by 3 publications

(2 citation statements)

References 139 publications

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“…MP-IVM uses a longer wavelength (700 nm and greater) than confocal techniques to penetrate more deep tissue, and could be used to monitor the pharmacological action of drugs in vivo ( 65 ). The MP-IVM obtains high-contrast cellular-level images from thick opaque specimens with minimized toxicity and photobleaching, and enables long-term imaging of biological processes in laboratory animals ( 66 ). MP-IVM was used to monitor leukocytes in vivo ( 67 ).…”

Section: In Vivo Single Cell Imagingmentioning

confidence: 99%

In vivo molecular and single cell imaging

Hong

Rhee

Jung

2022

BMB Rep

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Molecular imaging is used to improve the disease diagnosis, prognosis, monitoring of treatment in living subjects. Numerous molecular targets have been developed for various cellular and molecular processes in genetic, metabolic, proteomic, and cellular biologic level. Molecular imaging modalities such as Optical Imaging, Magnetic Resonance Imaging (MRI), Positron Emission Tomography (PET), Single Photon Emission Computed Tomography (SPECT), and Computed Tomography (CT) can be used to visualize anatomic, genetic, biochemical, and physiologic changes in vivo. For in vivo cell imaging, certain cells such as cancer cells, immune cells, stem cells could be labeled by direct and indirect labeling methods to monitor cell migration, cell activity, and cell effects in cell-based therapy. In case of cancer, it could be used to investigate biological processes such as cancer metastasis and to analyze the drug treatment process. In addition, transplanted stem cells and immune cells in cell-based therapy could be visualized and tracked to confirm the fate, activity, and function of cells. In conventional molecular imaging, cells can be monitored in vivo in bulk non-invasively with optical imaging, MRI, PET, and SPECT imaging. However, single cell imaging in vivo has been a great challenge due to an extremely high sensitive detection of single cell. Recently, there has been great attention for in vivo single cell imaging due to the development of single cell study. In vivo single imaging could analyze the survival or death, movement direction, and characteristics of a single cell in live subjects. In this article, we reviewed basic principle of in vivo molecular imaging and introduced recent studies for in vivo single cell imaging based on the concept of in vivo molecular imaging. [

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Section: In Vivo Single Cell Imagingmentioning

confidence: 99%

In vivo molecular and single cell imaging

Hong

Rhee

Jung

2022

BMB Rep

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“…This is where multiphoton microscopy beats the technology bottleneck. Liang and Walczak (Liang and Walczak, 2021) summarize the application of 2-photon microscopy for in vivo long-term single-cell tracking in the mouse and points out current challenges and opportunities for cell labeling and in vivo multiphoton-based imaging. They foresee a further expansion of this technology for the study of diseases, therapy and translational medicine.…”

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confidence: 99%

Optical and genetic tools for in vivo single cell tracking

Liang

Prida

2021

Journal of Neuroscience Methods

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Bioimaging approaches for quantification of individual cell behavior during cell fate decisions

Begnini

Pereira

Faccioni

et al. 2022

Biochemical Society Transactions

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Tracking individual cells has allowed a new understanding of cellular behavior in human health and disease by adding a dynamic component to the already complex heterogeneity of single cells. Technically, despite countless advances, numerous experimental variables can affect data collection and interpretation and need to be considered. In this review, we discuss the main technical aspects and biological findings in the analysis of the behavior of individual cells. We discuss the most relevant contributions provided by these approaches in clinically relevant human conditions like embryo development, stem cells biology, inflammation, cancer and microbiology, along with the cellular mechanisms and molecular pathways underlying these conditions. We also discuss the key technical aspects to be considered when planning and performing experiments involving the analysis of individual cells over long periods. Despite the challenges in automatic detection, features extraction and long-term tracking that need to be tackled, the potential impact of single-cell bioimaging is enormous in understanding the pathogenesis and development of new therapies in human pathophysiology.

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Long term intravital single cell tracking under multiphoton microscopy

Cited by 3 publications

References 139 publications

In vivo molecular and single cell imaging

In vivo molecular and single cell imaging

Optical and genetic tools for in vivo single cell tracking

Bioimaging approaches for quantification of individual cell behavior during cell fate decisions

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