Long-Term Productive Human Immunodeficiency Virus Infection of CD1a-Sorted Myeloid Dendritic Cells

Попов, Сергей Витальевич; Chenine, Agnès‐Laurence; Gruber, Andreas; Li, Peilin; Ruprecht, Ruth M.

doi:10.1128/jvi.79.1.602-608.2005

Cited by 39 publications

(29 citation statements)

References 36 publications

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“…While previous studies have investigated HIV infection of LCs, the ability of uninfected LCs to mediate transinfection of target cells remains unknown (2,22,23,30). We observed that activated LCs incubated with HIV were able to stimulate transinfection of target cells, while unactivated LCs were not.…”

mentioning

confidence: 71%

Activated CD34-Derived Langerhans Cells Mediate Transinfection with Human Immunodeficiency Virus

Fahrbach

Barry

Ayehunie

et al. 2007

J Virol

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mentioning

confidence: 71%

Activated CD34-Derived Langerhans Cells Mediate Transinfection with Human Immunodeficiency Virus

Fahrbach

Barry

Ayehunie

et al. 2007

J Virol

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“…Highly active antiretroviral therapy significantly suppresses the HIV viral load but is unable to eradicate the virus from the body, leaving the possibility of the persistence of viral reservoirs. HIV-infected DCs act as viral reservoirs for long-term HIV transmission in vivo [22,23,24]. Identifying ways to trigger innate and adaptive immune responses to inhibit DC-endorsed HIV replication represents a potential strategy to limit HIV infection.…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, HIV-1 can preferentially infect and replicate in DCs (cis-infection pathway) by exploiting innate immune signaling through TLR8 and DC-SIGN [18,19,20,21]. Increasing evidence indicates that HIV-infected DCs act as viral reservoirs for long-term HIV transmission in vivo [22,23,24]. Although DCs are susceptible to both R5 and X4 HIV-1 strains, HIV-1 replication is generally less productive in DCs than in CD4 + T cells, presumably because of the expression of restriction factors in DCs that block HIV-1 replication.…”

Section: Introductionmentioning

confidence: 99%

HIV-1 Gag-Virus-Like Particles Inhibit HIV-1 Replication in Dendritic Cells and T Cells through IFN-α-Dependent Upregulation of APOBEC3G and 3F

Chang

Suzuki²,

Yamamoto

et al. 2012

J Innate Immun

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Human immunodeficiency virus-1 (HIV-1) infection and the acquired immune deficiency syndrome (AIDS) pandemic remain global threats in the absence of a protective or a therapeutic vaccine. HIV-1 replication is reportedly inhibited by some cellular factors, including APOBEC3G (A3G) and APOBEC3F (A3F), which are well known inhibitors of HIV-1. Recently, HIV-1 Gag-virus-like particles (Gag-VLPs) have been shown to be safe and potent HIV-1 vaccine candidates that can elicit strong cellular and humoral immunity without need of any adjuvant. In this report, we stimulated human monocyte-derived dendritic cells (DCs) with Gag-VLPs and we demonstrated that Gag-VLP-treated DCs (VLP-DCs) produced interferon alpha (IFN-α), along with an increase in mRNA and protein expression of A3G and A3F. Gag-VLPs inhibited HIV-1 replication not only in DCs themselves, but also in cocultured T cells in an IFN-α-dependent manner. In addition, A3G/3F content in HIV virions released from VLP-DCs increased. Both the increase in A3G/3F expression and the inhibition of HIV-1 replication were reversed by anti-IFN-α or anti-IFNAR antibodies. Our findings in this study provide insight into the mechanism of Gag-VLP-induced inhibition of HIV-1 replication in DCs and T cells.

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“…As negative control, 293T cells infected with wt VV were transfected with plasmid pSC59. When GFP-positive cells appeared, cells were sorted by flow cytometry as described (Popov et al, 2005).…”

Section: Generation Of Recombinant Vvsmentioning

confidence: 99%

Generation of Recombinant Vaccinia Viruses via Green Fluorescent Protein Selection

Попов

Mirshahidi

Essono

et al. 2009

DNA and Cell Biology

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We developed a rapid method to generate recombinant vaccinia viruses (rVVs) based upon a bicistronic cassette encoding the gene for green fluorescent protein (GFP) and a foreign gene of interest separated by an internal ribosome entry site (IRES). As proof-of-concept, we inserted a mutant env gene of human immunodeficiency virus (HIV) into the cassette, which was cloned into the vaccinia virus (VV) insertion vector pSC59 under the control of the early-late VV synthetic promoter and flanked by disrupted tk gene sequences. To generate rVVs, 293T cells were inoculated with wild-type (wt) VV, followed by transfection of the modified pSC59 vector containing the bicistronic cassette, which allows expression of GFP and the protein of interest. Next, GFPpositive cells were isolated by flow cytometry or by picking under a fluorescent microscope. Thymidine kinasedeficient (Tk À ) 143B cells were then exposed to lysates of GFP-positive 293T cells and cultured in the presence of bromodeoxyuridine. This selection allows only Tk À rVV to remain viable. We demonstrated the success of this GFP selection strategy by expressing high levels of mutant HIV Env. Our approach shortens the time needed to generate rVVs and represents a practical approach to generate recombinant proteins.

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Long-Term Productive Human Immunodeficiency Virus Infection of CD1a-Sorted Myeloid Dendritic Cells

Cited by 39 publications

References 36 publications

Activated CD34-Derived Langerhans Cells Mediate Transinfection with Human Immunodeficiency Virus

Activated CD34-Derived Langerhans Cells Mediate Transinfection with Human Immunodeficiency Virus

HIV-1 Gag-Virus-Like Particles Inhibit HIV-1 Replication in Dendritic Cells and T Cells through IFN-α-Dependent Upregulation of APOBEC3G and 3F

Generation of Recombinant Vaccinia Viruses via Green Fluorescent Protein Selection

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