Long-term spatiotemporal stability and dynamic changes in helminth infracommunities of bank voles (<i>Myodes glareolus</i>) in NE Poland

Grzybek, Maciej; Bajer, Anna; Bednarska, Małgorzata; Alsarraf, Mohammed; Behnke‐Borowczyk, Jolanta; Harris, P. D.; Price, Stephen J.; Brown, Gabrielle S.; Osborne, Sarah-Jane; Siński, E.; Behnke, Jerzy M.

doi:10.1017/s0031182015001225

Cited by 42 publications

(93 citation statements)

References 64 publications

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“…The low number of differentially expressed genes is possibly caused by that animals with B. afzelii infections were in different stages of the infection, and/ or that some of the animals were infected with other pathogens (such as, "Candidatus Neoehrlichia mikurensis," Bartonella spp. and helminths (Buffet et al, 2012;Andersson, Bartkova, Lindestad, & Råberg, 2013;Grzybek et al, 2015)), all of which are common in the population studied here, and may trigger partly the same immune pathways as B. afzelii infection. These and other factors undoubtedly contribute noise to our data set.…”

Section: Discussionmentioning

confidence: 86%

Comparison of spleen transcriptomes of two wild rodent species reveals differences in the immune response against Borrelia afzelii

Zhong

Lundberg

Råberg

2020

Ecology and Evolution

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Different host species often differ considerably in susceptibility to a given pathogen, but the causes of such differences are rarely known. The natural hosts of the tick‐transmitted bacterium Borrelia afzelii, which is one of causative agents of Lyme borreliosis in humans, include a variety of small mammals like voles and mice. Previous studies have shown that B. afzelii‐infected bank voles (Myodes glareolus) have about ten times higher bacterial load than infected yellow‐necked mice (Apodemus flavicollis), indicating that these two species differ in resistance. In this study, we compared the immune response to B. afzelii infection in these host species by using RNA sequencing to quantify gene expression in spleen. Gene set enrichment analysis (GSEA) showed that several immune pathways were down‐regulated in infected animals in both bank voles and yellow‐necked mice. Moreover, IFNα response was up‐regulated in B. afzelii‐infected yellow‐necked mice, while IL6 signaling and the complement pathway were down‐regulated in infected bank voles; differences in regulation of these three pathways between bank voles and yellow‐necked mice could thus contribute to the difference in resistance to B. afzelii between the species. This study provides knowledge of gene expression induced by a zoonotic pathogen in its natural host, and possible species‐specific regulation of immune responses associated with resistance.

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Section: Discussionmentioning

confidence: 86%

Comparison of spleen transcriptomes of two wild rodent species reveals differences in the immune response against Borrelia afzelii

Zhong

Lundberg

Råberg

2020

Ecology and Evolution

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“…The adopted statistical approach has previously been described (Behnke et al, 2008;Grzybek et al, 2015Grzybek et al, , 2014. Briefly, for analysis of For analyses of the quantitative data we used general linear models (GLM) implemented in R version 2.2.1 (R Core Development Team).…”

Section: Discussionmentioning

confidence: 99%

Analysis of intrinsic and extrinsic factors influencing the dynamics of bovine Eimeria spp. from central–eastern Poland

Tomczuk

Grzybek

Szczepaniak

et al. 2015

Veterinary Parasitology

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“…The statistical approach adopted has been documented comprehensively in our earlier publications [35, 38–40]. Prevalence (percentage of animals infected) was analyzed by maximum likelihood techniques based on log-linear analysis of contingency tables (in SPSS v. 21).…”

Section: Methodsmentioning

confidence: 99%

Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success

et al. 2018

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BackgroundBartonella spp. cause persistent bacterial infections in mammals. Although these bacteria are transmitted by blood-feeding arthropods, there is also evidence for vertical transmission in their mammalian hosts. We aimed to determine: (i) the prevalence and diversity of Bartonella spp. in a Microtus spp. community; (ii) whether vertical transmission occurs from infected female voles to their offspring; (iii) the effect of concurrent Babesia microti infection on the success of vertical transmission of Bartonella; and (iv) the impact of congenital infection on pup survival.ResultsWe sampled 124 Microtus arvalis, 76 Microtus oeconomus and 17 Microtus agrestis. In total, 115 embryos were isolated from 21 pregnant females. In the following year 11 pregnant females were kept until they had given birth and weaned their pups (n = 62). Blood smears and PCR targeting the Bartonella-specific rpoB gene fragment (333bp) were used for the detection of Bartonella. Bartonella DNA was detected in 66.8% (145/217) of the wild-caught voles. Bartonella infection was detected in 81.8% (36/44) of pregnant female voles. Bartonella-positive individuals were identified among the embryos (47.1%; 40/85) and in 54.8% (34/62) of pups. Congenitally acquired Bartonella infections and co-infection with B. microti had no impact on the survival of pups over a 3-week period post partum. Among 113 Bartonella sequences, four species were detected: Bartonella taylorii, Bartonella grahamii, Bartonella doshiae and a Bartonella rochalimae-like genotype. Bartonella taylorii clade B was the dominant species in wild-caught voles (49%), pregnant females (47%), their embryos (85%), dams (75%) and pups (95%).ConclusionsHigh prevalence of Bartonella spp. infection maintained in Microtus spp. community is followed by a high rate of vertical transmission of several rodent species of Bartonella in three species of naturally infected voles, M. arvalis, M. oeconomus and M. agrestis. Congenitally acquired Bartonella infection does not affect the survival of pups. Co-infection with B. microti does not affect the effectiveness of the vertical transmission of Bartonella in voles. Bartonella taylorii clade B was found to be the dominant species in wild-caught voles, including pregnant females and dams, and in their offspring, and was also found to be the most successful in vertical transmission.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3047-6) contains supplementary material, which is available to authorized users.

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Long-term spatiotemporal stability and dynamic changes in helminth infracommunities of bank voles (Myodes glareolus) in NE Poland

Cited by 42 publications

References 64 publications

Comparison of spleen transcriptomes of two wild rodent species reveals differences in the immune response against Borrelia afzelii

Comparison of spleen transcriptomes of two wild rodent species reveals differences in the immune response against Borrelia afzelii

Analysis of intrinsic and extrinsic factors influencing the dynamics of bovine Eimeria spp. from central–eastern Poland

Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success

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