Longitudinal analysis of serological tests officially adopted by the Brazilian Ministry of Health for the diagnosis of canine visceral leishmaniasis in dogs vaccinated with Leishmune®

Marcondes, Mary; Lima, Valéria Marçal Felix de; Araújo, María de Fátima; Hiramoto, Roberto Mitsuyoshi; Tolezano, José Eduardo; Vieira, Rafael Felipe da Costa; Biondo, Alexander Welker

doi:10.1016/j.vetpar.2013.07.013

Cited by 27 publications

(31 citation statements)

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“…Cross-reactivity in Leishmune ® -vaccinated dogs, as revealed by official serological tests, has also been recorded by Araújo (2006) and Andrade et al (2009). According to these researchers, seropositivity is typically detected in assessments performed near to the time of vaccination, but such reactivity tends to diminish with time (Araújo 2006;Andrade et al 2009;Marcondes et al 2011Marcondes et al , 2013. In the light of this evidence, it is important to monitor vaccinated dogs for an extended period.…”

Section: Discussionmentioning

confidence: 90%

“…Furthermore, Marcondes et al (2011) stated that commercial polyclonal antibodies for total IgG, IgG1 and IgG2 are not able to distinguish infected and vaccinated dogs. The same group (Marcondes et al 2013) has recently reported that an in-house ELISA, as well as the official serological tests adopted by the Brazilian Ministry of Health, exhibit crossreactivity up to 6 months after the first dose of vaccine. Cross-reactivity in Leishmune ® -vaccinated dogs, as revealed by official serological tests, has also been recorded by Araújo (2006) and Andrade et al (2009).…”

Section: Discussionmentioning

confidence: 94%

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Ability of immunodiagnostic tests to differentiate between dogs naturally infected with Leishmania infantum and Leishmune®-vaccinated dogs

et al. 2015

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Visceral leishmaniasis (VL) is a serious chronic disease with a lethality rate of up to 10% in humans. In urban areas of Brazil, dogs are the main reservoirs of the etiological agent (Leishmania infantum) of VL, and the Brazilian Ministry of Health recommends the euthanasia of animals that are seropositive in both the immunochromatographic dual path platform rapid test (DPP(®); Bio-Manguinhos) and the enzyme-linked immunosorbent assay (ELISA) with an L. major-like antigen (Bio-Manguinhos). Vaccination is an additional tool in the control of canine VL, but the use of Leishmune(®) (Zoetis Indústria de Produtos Veterinários, São Paulo, SP, Brazil), which contains the fucose mannose ligand (FML) isolated from L. donovani, is not currently recommended by the Brazilian Ministry of Health because vaccinated animals may exhibit positive serology and there are reservations regarding the efficacy of the vaccine. The aims of the present study were: (i) to verify the abilities of the fast agglutination screening test (FAST), the direct agglutination test (DAT), the indirect fluorescent-antibody test (IFAT), the DPP rapid test, and ELISA tests with L. major-like and FML antigens to differentiate between L. infantum-infected and Leishmune(®)-vaccinated dogs, and (ii) to analyze the sensitivities and specificities of the different methods. The reactivities to these tests of Leishmune(®)-vaccinated dogs (n = 71), asymptomatic (n = 20) and symptomatic (n = 20) naturally infected dogs, and unvaccinated healthy control dogs (n = 5) were compared. None of the Leishmune(®)-vaccinated dogs tested seropositive in FAST and DAT, although one dog was reactive to DPP and four dogs to ELISA/L. major-like and IFAT tests. While 69 (97%) of vaccinated dogs reacted to ELISA/FML, only one was seropositive in both ELISA/L. major-like and IFAT tests. Individually, all immunodiagnostic tests presented high specificities and positive likelihood ratios (LR+), and high specificity values were obtained when the tests were considered in pairs. However, sensitivity and LR- values were low for ELISA/L. major-like and IFAT tests individually, and for all pair combinations of tests except for FAST with DPP.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 94%

Ability of immunodiagnostic tests to differentiate between dogs naturally infected with Leishmania infantum and Leishmune®-vaccinated dogs

et al. 2015

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“…Previous studies have shown higher levels of seroconversion to the MH tests (SOLCÀ et al 2013;MARCONDES et al, 2013); however, the highest seroconversion rate has always been verified in the EIE, formulated with complete L. major antigen promastigotes, presenting low concentrations of vaccine antigens, which would justify these findings.…”

Section: Discussionmentioning

confidence: 93%

“…Similarly, NOGUEIRA (2005) observed seropositivity of 100% in dogs immunized with Leishmune ® for up to 11 months. With respect to the positivity assessment of the tests used by the MH, seroconversion in dogs vaccinated with Leishmune ® was reported in DPP and EIE 6 months after the vaccination protocol (MARCONDES et al, 2013). In contrast, dogs immunized with LeishTec ® , monitored up to 14 months after the priming vaccination, did not present seroconversion in the MH tests (TESTASICCA et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Can vaccines against canine visceral leishmaniasis interfere with the serological diagnostics recommended by the Brazilian Ministry of Health?

et al. 2017

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The objective of the current research was to assess seroconversion in dogs immunized with Leishmune® and Leish Tec® vaccines using rapid chromatographic immunoassay DPP® (Dual Path Platform) (DPP CVL) and enzyme immunoassay (EIE) up to one year after the vaccination protocol. The study sample comprised 28 dogs divided into two groups, each group immunized with an anti-CVL vaccine and clinically monitored for one year through clinical evaluation and laboratory tests. 22 (78.5%) dog were monitored. During the evaluation time (T1-30 days, T2-6 months, and T3-1 year after vaccination) the results for all dogs were negative for CVL, except for one animal vaccinated with Leish tec® that seroconverted in the DPP CVL test at T2. Subsequent examinations of this dog were negative. Our results showed that in a non-endemic area, even at different evaluation times, dogs vaccinated against CVL with Leishmune® or Leish tec® did not seroconvert in the serological protocol used by the Brazilian Ministry of Health (DPP/EIE).

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“…Serological assays based on the detection of antibodies reactive with recombinant proteins and rapid in-house tests are usually less sensitive for the recognition of antibodies elicited by vaccination than those based on whole-parasite antigens and quantitative serological techniques (Marcondes and others 2013, Moreno and others 2014, Starita and others 2016). The rapid serological test Speed Leish K (Virbac) detects circulating antibodies against kinesins of L infantum and might be used to distinguish between vaccinated and naturally infected dogs.…”

Section: Diagnosismentioning

confidence: 99%

Leishmaniosis in dogs and cats

Silvestrini¹

2019

In Practice

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Canine leishmaniosis is pushing northwards out of its traditional endemic regions as the distribution of its vector expands and increasing numbers of dogs travel between countries. In non-endemic areas, imported disease represents a threat to animal and human health. This article provides an understanding of the epidemiology, life cycle and transmission of Leishmania infantum, reviews its diagnosis and treatment, and briefly discusses the current understanding of feline leishmaniosis.

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Longitudinal analysis of serological tests officially adopted by the Brazilian Ministry of Health for the diagnosis of canine visceral leishmaniasis in dogs vaccinated with Leishmune®

Cited by 27 publications

References 11 publications

Ability of immunodiagnostic tests to differentiate between dogs naturally infected with Leishmania infantum and Leishmune®-vaccinated dogs

Ability of immunodiagnostic tests to differentiate between dogs naturally infected with Leishmania infantum and Leishmune®-vaccinated dogs

Can vaccines against canine visceral leishmaniasis interfere with the serological diagnostics recommended by the Brazilian Ministry of Health?

Leishmaniosis in dogs and cats

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