Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy

Won, Jungeun; Hong, Wenzhou; Khampang, Pawjai; Spillman, Darold R.; Marshall, Samuels; Yan, Ke; Porter, Ryan G.; Novák, Michael; Kerschner, Joseph E.; Boppart, Stephen A.

doi:10.1038/s41598-021-84543-9

Cited by 17 publications

(9 citation statements)

References 45 publications

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“…As a next step, we will employ a 3D bio-printed eardrum model or implantable eardrum scaffolds that highly resemble the human tympanic membrane so as to better represent and examine the structures and properties, including tissue permeability. In vivo preclinical models, such as otitis media-induced chinchilla models, will also be helpful to evaluate the efficacy of microplasma on naturally grown middle ear biofilm that is adhered to the eardrum 48 , as well as on the mucosal membrane. Different designs of microplasma delivery platforms developed in the present study (Fig.…”

Section: Discussionmentioning

confidence: 99%

Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media

Sun

Won

Choo-Kang

et al. 2021

npj Biofilms Microbiomes

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Otitis media (OM), known as a middle ear infection, is the leading cause of antibiotic prescriptions for children. With wide-spread use of antibiotics in OM, resistance to antibiotics continues to decrease the efficacy of the treatment. Furthermore, as the presence of a middle ear biofilm has contributed to this reduced susceptibility to antimicrobials, effective interventions are necessary. A miniaturized 3D-printed microplasma jet array has been developed to inactivate Pseudomonas aeruginosa, a common bacterial strain associated with OM. The experiments demonstrate the disruption of planktonic and biofilm P. aeruginosa by long-lived molecular species generated by microplasma, as well as the synergy of combining microplasma treatment with antibiotic therapy. In addition, a middle ear phantom model was developed with an excised rat eardrum to investigate the antimicrobial effects of microplasma on bacteria located behind the eardrum, as in a patient-relevant setup. These results suggest the potential for microplasma as a new treatment paradigm for OM.

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Section: Discussionmentioning

confidence: 99%

Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media

Sun

Won

Choo-Kang

et al. 2021

npj Biofilms Microbiomes

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“…Demonstrations of (partly) miniaturized OCT systems in research have been accomplished in ophthalmic care using handheld probes, 330,[334][335][336][337][338][339][340][341][342][343] off-the-shelf micro-optical components 52,334,344 or PICs 329,345,346 for dermatologic care using handheld probes, [347][348][349][350][351][352][353] compact packaging of offthe-shelf components, 354 PICs, 353,[355][356][357] and handheld probes for oral 358 and for inner ear [359][360][361][362] imaging. The usage of off-the-shelf microcomponents seems to be the most mature technique for miniaturized OCT at the moment.…”

Section: Oct and Deep Learning Neural Network And Artificial Intelligencementioning

confidence: 99%

Enhanced medical diagnosis for dOCTors: a perspective of optical coherence tomography

et al. 2021

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Significance: After three decades, more than 75,000 publications, tens of companies being involved in its commercialization, and a global market perspective of about USD 1.5 billion in 2023, optical coherence tomography (OCT) has become one of the fastest successfully translated imaging techniques with substantial clinical and economic impacts and acceptance.Aim: Our perspective focuses on disruptive forward-looking innovations and key technologies to further boost OCT performance and therefore enable significantly enhanced medical diagnosis.Approach: A comprehensive review of state-of-the-art accomplishments in OCT has been performed. Results:The most disruptive future OCT innovations include imaging resolution and speed (single-beam raster scanning versus parallelization) improvement, new implementations for dual modality or even multimodality systems, and using endogenous or exogenous contrast in these hybrid OCT systems targeting molecular and metabolic imaging. Aside from OCT angiography, no other functional or contrast enhancing OCT extension has accomplished comparable clinical and commercial impacts. Some more recently developed extensions, e.g., optical coherence elastography, dynamic contrast OCT, optoretinography, and artificial intelligence enhanced OCT are also considered with high potential for the future. In addition, OCT miniaturization for portable, compact, handheld, and/or cost-effective capsule-based OCT applications, home-OCT, and self-OCT systems based on micro-optic assemblies or photonic integrated circuits will revolutionize new applications and availability in the near future. Finally, clinical translation of OCT including medical device regulatory challenges will continue to be absolutely essential.Conclusions: With its exquisite non-invasive, micrometer resolution depth sectioning capability, OCT has especially revolutionized ophthalmic diagnosis and hence is the fastest adopted imaging technology in the history of ophthalmology. Nonetheless, OCT has not been completely exploited and has substantial growth potential-in academics as well as in industry. This applies not only to the ophthalmic application field, but also especially to the original motivation of OCT to enable optical biopsy, i.e., the in situ imaging of tissue microstructure with a resolution approaching that of histology but without the need for tissue excision.

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“…Optical coherence tomography (OCT) is one such technique that provides label-free 2D cross-sectional images of samples and tissue. OCT has been widely used in the eye (retina) 11 and heart 12 for diagnostics and visualization, and for imaging biofilms to characterize biofilm growth, porosity, and mechanical properties 13 , 14 on both the bench 15 and in vivo 16 .…”

Section: Introductionmentioning

confidence: 99%

Texture-based speciation of otitis media-related bacterial biofilms from optical coherence tomography images using supervised classification.

Boppart,

Zaki,

Monroy

et al. 2023

Preprint

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Otitis media (OM) is primarily a bacterial middle-ear infection prevalent among children worldwide. In recurrent and/or chronic OM cases, antibiotic-resistant bacterial biofilms can develop in the middle ear. A biofilm related to OM typically contains one or multiple bacterial strains, the most common include Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, Pseudomonas aeruginosa, and Staphylococcus aureus. Optical coherence tomography (OCT) has been used clinically to visualize the presence of bacterial biofilms in the middle ear. This study used OCT to compare microstructural image texture features from primary bacterial biofilms in vitro and in vivo. The proposed method applied supervised machine-learning-based frameworks (SVM, random forest (RF), and XGBoost) to classify and speciate multiclass bacterial biofilms from the texture features extracted from OCT B-Scan images obtained from in vitro cultures and from clinically-obtained in vivo images from human subjects. Our findings show that optimized SVM-RBF and XGBoost classifiers can help distinguish bacterial biofilms by incorporating clinical knowledge into classification decisions. Furthermore, both classifiers achieved more than 95% of AUC (area under receiver operating curve), detecting each biofilm class. These results demonstrate the potential for differentiating OM-causing bacterial biofilms through texture analysis of OCT images and a machine-learning framework, which could provide additional clinically relevant data during real-time in vivo characterization of ear infections.

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Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy

Cited by 17 publications

References 45 publications

Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media

Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media

Enhanced medical diagnosis for dOCTors: a perspective of optical coherence tomography

Texture-based speciation of otitis media-related bacterial biofilms from optical coherence tomography images using supervised classification.

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