Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice

Hattori, Ryoma; Komiyama, Takaki

doi:10.1016/j.xpro.2022.101343

Cited by 17 publications

(10 citation statements)

References 18 publications

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“…We used a projector system ( 9 , 12 , 13 ) to apply blue light over each cortical area. This flexible light delivery system, combined with a large cranial window preparation ( 14 ), allowed us to investigate the role of multiple cortical areas separately within the same animal (schematic in Fig. 4A ).…”

Section: Resultsmentioning

confidence: 99%

“…Surgery for calcium imaging and optogenetic inactivation. Animals were prepared for imaging and optogenetic experiments with a large cranial window placed over dorsal cortex, as previously reported in (9,14). Briefly, mice were anesthetized with 1 to 2% isoflurane during surgery, the dorsal surface of the skull was exposed and cleared of soft tissue with a razor blade and marked with the coordinates of interest.…”

Section: Methods Detailsmentioning

confidence: 99%

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Exponential history integration with diverse temporal scales in retrosplenial cortex supports hyperbolic behavior

Danskin,

Hattori,

Zhang

et al. 2023

Sci. Adv.

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Animals use past experience to guide future choices. The integration of experiences typically follows a hyperbolic, rather than exponential, decay pattern with a heavy tail for distant history. Hyperbolic integration affords sensitivity to both recent environmental dynamics and long-term trends. However, it is unknown how the brain implements hyperbolic integration. We found that mouse behavior in a foraging task showed hyperbolic decay of past experience, but the activity of cortical neurons showed exponential decay. We resolved this apparent mismatch by observing that cortical neurons encode history information with heterogeneous exponential time constants that vary across neurons. A model combining these diverse timescales recreated the heavy-tailed, hyperbolic history integration observed in behavior. In particular, the time constants of retrosplenial cortex (RSC) neurons best matched the behavior, and optogenetic inactivation of RSC uniquely reduced behavioral history dependence. These results indicate that behavior-relevant history information is maintained across multiple timescales in parallel and that RSC is a critical reservoir of information guiding decision-making.

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Section: Resultsmentioning

confidence: 99%

Section: Methods Detailsmentioning

confidence: 99%

Exponential history integration with diverse temporal scales in retrosplenial cortex supports hyperbolic behavior

Danskin,

Hattori,

Zhang

et al. 2023

Sci. Adv.

Self Cite

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show abstract

“…We provide detailed schematics and instructions for the preparation and installation of the COMBO window, as well as for the cranial window surgery itself ( Appendix 1, Supplementary Figure 2 ), to encourage optimal and consistent use. For additional reference, details regarding cranial window surgery and the challenges associated with large skull openings have been described elsewhere 23,24 .…”

Section: Resultsmentioning

confidence: 99%

The COMBO window: A chronic cranial implant for multiscale circuit interrogation in mice

Edelman,

Siegenthaler,

Wanken

et al. 2023

Preprint

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Neuroscientists studying the neural correlates of mouse behavior often lack access to the brain-wide activity patterns elicited during a specific task of interest. Fortunately, large-scale imaging is becoming increasingly accessible thanks to modalities such as Ca2+imaging and functional ultrasound (fUS). However, these and other techniques often involve challenging cranial window procedures, and are difficult to combine with other neuroscience tools. We address this need with an open-source 3D-printable cranial implant - the COMBO (ChrOnicMultimodal imaging andBehavioralObservation) window. The COMBO window enables chronic imaging of large portions of the brain in head-fixed mice while preserving orofacial movements. We validate the COMBO window stability using both brain-wide fUS and multi-site two-photon imaging. Moreover, we demonstrate how the COMBO window facilitates the combination of optogenetics, fUS and electrophysiology in the same animals to study the effects of circuit perturbations at both the brain-wide and single-neuron level. Overall, the COMBO window provides a versatile solution for performing multimodal brain recordings in head-fixed mice.

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“…The cranial implant for imaging contained a 6 x 6 mm glass plug, adapted from (Hattori & Komiyama) 45 , that compressed a section of the dorsal cortex (-4 to +2 mm AP, -3 to +3 mm ML) into a single flat imaging plane. The glass plug was assembled from two pieces of glass.…”

Section: Imaging Cranial Implantmentioning

confidence: 99%

Brain-wide neural recordings in mice navigating physical spaces enabled by a cranial exoskeleton

Kodandaramaiah,

Hope,

Beckerle

et al. 2023

Preprint

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Complex behaviors are mediated by neural computations occurring throughout the brain. In recent years, tremendous progress has been made in developing technologies that can record neural activity at cellular resolution at multiple spatial and temporal scales. However, these technologies are primarily designed for studying the mammalian brain during head fixation – wherein the behavior of the animal is highly constrained. Miniaturized devices for studying neural activity in freely behaving animals are largely confined to recording from small brain regions owing to performance limitations. We present a cranial exoskeleton that assists mice in maneuvering neural recording headstages that are orders of magnitude larger and heavier than the mice, while they navigate physical behavioral environments. Force sensors embedded within the headstage are used to detect the mouse’s milli-Newton scale cranial forces which then control the x, y, and yaw motion of the exoskeleton via an admittance controller. We discovered optimal controller tuning parameters that enable mice to locomote at physiologically realistic velocities and accelerations while maintaining natural walking gait. Mice maneuvering headstages weighing up to 1.5 kg can make turns, navigate 2D arenas, and perform a navigational decision-making task with the same performance as when freely behaving. We designed an imaging headstage and an electrophysiology headstage for the cranial exoskeleton to record brain-wide neural activity in mice navigating 2D arenas. The imaging headstage enabled recordings of Ca2+ activity of 1000s of neurons distributed across the dorsal cortex. The electrophysiology headstage supported independent control of up to 4 silicon probes, enabling simultaneous recordings from 100s of neurons across multiple brain regions and multiple days. Cranial exoskeletons provide flexible platforms for largescale neural recording during the exploration of physical spaces, a critical new paradigm for unraveling the brain-wide neural mechanisms that control complex behavior.

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Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice

Cited by 17 publications

References 18 publications

Exponential history integration with diverse temporal scales in retrosplenial cortex supports hyperbolic behavior

Exponential history integration with diverse temporal scales in retrosplenial cortex supports hyperbolic behavior

The COMBO window: A chronic cranial implant for multiscale circuit interrogation in mice

Brain-wide neural recordings in mice navigating physical spaces enabled by a cranial exoskeleton

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