Longitudinally cultured precision cut lung slices as a predictiveex vivomodel for lung cancer

Abstract

Exploring alternatives toin vivomodels, this study validates Precision Cut Lung Slices (PCLS) as a viableex vivoplatform for lung cancer research. We established the prolonged viability and structural preservation of PCLS, essential for accurate drug response studies. Using Paclitaxel as a benchmark drug and a therapeutically promising silver nanoparticles in combination with immunotherapy, we conducted a pioneering comparative analysis of its therapeutic effects on PCLS against traditionalin vivomodels. Results revealed that PCLS closely mimicsin vivoresponses, demonstrating comparable drug efficacy in tumor growth inhibition. This direct comparison not only confirms the utility of PCLS in simulating real-world outcomes but also emphasizes its potential in reducing animal testing. By providing a reliable, ethical, and efficient alternative for lung cancer studies, PCLS could significantly enhance preclinical research and drug development, marking a critical step towards more humane and representative scientific investigations.