Looking beyond the skin: Cutaneous and systemic oxidative stress in UVB-induced squamous cell carcinoma in hairless mice

Carrara, Iriana Moratto; Melo, Gabriella Pasqual; Bernardes, Sara Santos; Neto, Fernando Souza; Ramalho, Leandra Náira Zambelli; Marinello, Poliana Camila; Luiz, Rodrigo Cabral; Cecchini, Rúbens; Cecchini, Alessandra Lourenço

doi:10.1016/j.jphotobiol.2019.04.007

Cited by 21 publications

(22 citation statements)

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“…In the following weeks, the skin lesions were subjected to plasma treatment in alternated days with continued UVB irradiation to see whether plasma treatment alters the development of the disease ( Figure 2 C). After 4 weeks of plasma treatment, the animals were euthanized and the skin lesions and their progression status were analyzed via histopathology, according to standardized parameters, as previously described [ 33 ]. Unlike the control groups ( Figure 3 A,B), the UVB irradiation induced an increase of epidermal thickness ( Figure 3 C,E).…”

Section: Resultsmentioning

confidence: 99%

“…Several risk factors have been described for the development of SCC, but exposure to UVB irradiation for many decades that stands out as the most harmful [ 44 ]. Among the UV spectrum, UVB has higher energy levels than UVA and typically damages the outermost layers of the skin, interacting directly and indirectly with DNA [ 45 ], eventually causing skin cancer [ 33 ]. Our study showed that the animals developed a macroscopic skin lesion with histopathological characteristics similar to those of SCC after 7 weeks of UVB-irradiation.…”

Section: Discussionmentioning

confidence: 99%

“… Histopathological evaluation. ( A – D ) Representative images of murine skin sections stained with hematoxylin and eosin (H&E) from the untreated (control) ( A ), plasma ( B ), UVB ( C ), and UVB + plasma ( D ) treated mice, respectively (40×); ( E ) quantification of the epidermal thickness (see black arrow in C as an example); ( F ) histopathological score of characteristics related to SCC, which includes follicular atrophy, dermal degeneration, and keratin pearls (see black square in C as an example) [ 33 ]. The black circles ( C ) indicate nests of dysplastic squamous cells reaching the dermis.…”

Section: Figurementioning

confidence: 99%

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Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

Pasqual-Melo

Nascimento

Sanches

et al. 2020

Cancers

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Cutaneous squamous cell carcinoma (SCC) is the most prevalent cancer worldwide, increasing the cost of healthcare services and with a high rate of morbidity. Its etiology is linked to chronic ultraviolet (UV) exposure that leads to malignant transformation of keratinocytes. Invasive growth and metastasis are severe consequences of this process. Therapy-resistant and highly aggressive SCC is frequently fatal, exemplifying the need for novel treatment strategies. Cold physical plasma is a partially ionized gas, expelling therapeutic doses of reactive oxygen and nitrogen species that were investigated for their anticancer capacity against SCC in vitro and SCC-like lesions in vivo. Using the kINPen argon plasma jet, a selective growth-reducing action of plasma treatment was identified in two SCC cell lines in 2D and 3D cultures. In vivo, plasma treatment limited the progression of UVB-induced SSC-like skin lesions and dermal degeneration without compromising lesional or non-lesional skin. In lesional tissue, this was associated with a decrease in cell proliferation and the antioxidant transcription factor Nrf2 following plasma treatment, while catalase expression was increased. Analysis of skin adjacent to the lesions and determination of global antioxidant parameters confirmed the local but not systemic action of the plasma anticancer therapy in vivo.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

Pasqual-Melo

Nascimento

Sanches

et al. 2020

Cancers

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“…An inadequate immune defense can cause some types of dermatitis. Skin inflammation is closely related to the levels of cytokines and reactive oxygen species [ 56 ]. The essential oil from Curcuma longa (EOCl) [ 57 ] significantly reduced the levels of TNF- α , IL-6, and IL-1 in inflammatory models.…”

Section: Resultsmentioning

confidence: 99%

A Systematic Review of the Anti‐Inflammatory and Immunomodulatory Properties of 16 Essential Oils of Herbs

Zuo

Wang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Inflammation is a host defense mechanism in the body after it is infected and damaged. If inflammation is not treated in time, then it may cause a variety of diseases, such as cancer and autoimmune diseases. Herbal essential oils are natural extracts that can suppress inflammation effectively and are expected to be used in therapeutic drugs for anti-inflammatory diseases in the future. Aim of the review. We review the anti-inflammatory and immunomodulatory effects of essential oils derived from 16 herbs. Materials and methods. We searched the literature of the fields of anti-inflammatory and immunomodulatory herbal essential oil activity published in English within the past five years via databases (PubMed, EMBASE, Scopus, and The Web of Science). Results. A total of 1932 papers were found by searching, and 132 papers were screened after removing duplicates and reading article titles. Fifteen articles met the requirements to be included in this review. Among those selected, 11 articles reported in vivo research results, and 10 articles showed research results. Conclusion. Essential oils extracted from herbs can reduce inflammation by regulating the release of inflammatory cytokines involved in multiple signalling pathways. Herbal essential oils are expected to be developed as anti-inflammatory drugs.

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“…Chronic UVB radiation on skin causes accumulation of ROS, adjacent and tumoral oxidative stress, and oxidative damage [31]. As previously investigated by analytical methods, CS itself or its components exert antioxidant capacities [15,16,17,32].…”

Section: Discussionmentioning

confidence: 99%

Potential Photoprotective Effect of Dietary Corn Silk Extract on Ultraviolet B-Induced Skin Damage

et al. 2019

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Ultraviolet B (UVB) irradiation causes adverse effects on the skin. Corn silk contains flavonoids and other bioactive compounds and antioxidants, which may prevent skin photoaging through antioxidant and anti-inflammatory effects. We aimed to investigate the potential photoprotective effects of dietary corn silk on UVB-induced skin damage in mice and the mechanisms behind these effects on human skin cells. Oral administration of corn silk water extract (CS) (2 or 4 g/kg/day) for 19 weeks decreased epidermal thickness, wrinkle formation, and positive staining for PCNA, Ki67, and 8-OHdG, and increased collagen staining in UVB-irradiated SKH-1 hairless mice compared with controls. The pro-inflammatory NF-κB target genes (IL-1β, iNOS, and COX-2) and MMP-9 expressions were lower in the CS groups, and TGF-β/Smad signaling increased. Low skin lipid peroxidation and blood DNA oxidation levels and high blood glutathione were detected. Antioxidant transcription factor Nrf2-related catalase and SOD1 proteins and glutaredoxin mRNA levels increased. The results of CS extract treatment and UVB irradiation in HaCaT cells showed the same results in Nrf2 and NF-κB target genes. An LC-MS/MS analysis showed that the CS extract contained potential antioxidants, which might have contributed to its anti-photoaging effects in tissues and cells. CS extract may reduce UVB-induced skin damage through antioxidant and anti-inflammatory mechanisms.

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Looking beyond the skin: Cutaneous and systemic oxidative stress in UVB-induced squamous cell carcinoma in hairless mice

Cited by 21 publications

References 64 publications

Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

Plasma Treatment Limits Cutaneous Squamous Cell Carcinoma Development In Vitro and In Vivo

A Systematic Review of the Anti‐Inflammatory and Immunomodulatory Properties of 16 Essential Oils of Herbs

Potential Photoprotective Effect of Dietary Corn Silk Extract on Ultraviolet B-Induced Skin Damage

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