Looking for a way forward for the cryopreservation of orchid diversity

Das, Meera Chettri; Kumaria, Suman; Reed, Barbara M.

doi:10.1016/j.cryobiol.2021.05.004

Cited by 7 publications

(1 citation statement)

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“…Additionally, there is also an important methodological difficulty, as experimental protocol steps tested on one or a few genotypes in one genus may not work with others [ 7 ]. Given the wide diversity of orchids and the progress in orchid cryopreservation, Das et al [ 72 ] concluded that it would be useful to investigate any correlations between taxon and explants and cryopreservation procedures. Due to the small number of orchid species that have been cryopreserved, this type of comparison is challenging.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cryopreservation Method Supported with Biochemical Analyses in the Axillary Bud of Jewel Orchid, Ludisia discolor

Burkhan

Rajan

Appalasamy

et al. 2022

Plants

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This study investigated conserving an endangered terrestrial jewel orchid Ludisia discolor, using in vitro grown axillary buds. Excised segments of axillary buds (4–5 mm in length) were precultured on a modified Murashige and Skoog (MS) medium supplemented with 0.2 M sucrose for 24 h and osmoprotected in a loading solution for 20 min. Then, axillary buds were dehydrated in plant vitrification solution 2 (PVS2) for 10 min at 0 °C and incubated in liquid nitrogen for 1 h. Subsequently, axillary buds were rewarmed rapidly by dilution solution and transferred to a growth recovery medium supplemented with 0.05 µM melatonin, which led to an improved survival chance (16.67%) for cryopreserved L. discolor. The osmotic stress and the overproduction of reactive oxygen species (ROS) during cryopreservation stages may result in cryoinjuries and poor survival as increased levels of proline (5.51 µmol/g), catalase (85.64 U/g), peroxidase (565.37 U/g), and ascorbate peroxidase activities (12.19 U/g) were detected after dehydration, preculture, rewarming, and loading stage, respectively. Results obtained from this study indicate that further experimental designs which apply different PVS and exogenous antioxidants are needed for improved survival and regrowth of L. discolor.

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Section: Discussionmentioning

confidence: 99%