Loop-mediated isothermal amplification: an effective method for express-diagnostics of cancer

Abstract

The review is devoted to loop-mediated isothermal amplification (LAMP) – a novel molecular diagnostic method that has recently become increasingly popular. Unlike polymerase chain reaction, LAMP does not require thermal cycling; DNA or RNA amplification occurs at a constant temperature (about 65 °C) with 4 or 6 primers. This is a fast, highly-sensitive, and highly specific method, which does not require expensive equipment, where visual detection of the reaction products is performed by the unaided eye. LAMP is successfully used for the diagnosis of multiple viruses, bacteria, and other pathogens (including those in food). Moreover, it can be applied for the detection of singlenucleotide polymorphisms. Recently, a modified LAMP assay – one-step nucleic acid amplification (OSNA) – was validated for metastasis detection. OSNA was demonstrated to have almost the same sensitivity and specificity as standard diagnostic methods (sometimes even higher). Particular attention is paid to the mechanism of LAMP, primer design, and diagnostics of cancer using OSNA.

Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis

“…The sensitivity of LAMP was found to be an order of magnitude higher that the sensitivity of PCR. Moreover, LAMP is less sensitive to the inhibitors present in biological samples [17]. A serious drawback of this method is a high risk of contamination, often leading to the generation of false positives in negative controls [18].…”

Loop-Mediated Isothermal Amplification as a Promising Method for Mass COVID-19 Diagnostics

“…The primers were chosen in such a way as to ensure the formation of a hairpin structure at the beginning of amplification, based on which numerous concatemers containing the primary amplicon subsequently form (for detail, see review [ 27 ]). The only function of the outer primers is the formation of the hairpin structure, and its further amplification is carried out with the involvement of FIP and BIP primers.…”