Loop-mediated isothermal amplification for diagnosis of 18 World Organization for Animal Health (OIE) notifiable viral diseases of ruminants, swine and poultry

Mansour, S.; Ali, Haytham; Chase, Christopher; Cepica, Arnost

doi:10.1017/s1466252315000018

Cited by 47 publications

(35 citation statements)

References 84 publications

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“…Advances in field of diagnosis for the development of sensitive and specific point-of-care diagnostic assays like LAMP, lateral flow assay, recombinant protein based diagnostics, biosensors, biochips, microarrays, genomic fingerprinting and nanodiagnostics need to be explored to their full potential for diagnosing DEV (Belak et al 2009;Ji et al 2009;Balamurugan et al 2010;Ayyar & Arora, 2013;Num & Useh, 2013;Mansour et al 2015). These assays can aid in swift diagnosis of the disease thereby preventing the spread of virus and alleviating economical loss to the farmer.…”

Section: Molecular Diagnosis Of Devmentioning

confidence: 99%

Duck virus enteritis (duck plague) – a comprehensive update

Dhama

Kumar

Saminathan

et al. 2017

Veterinary Quarterly

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Duck virus enteritis (DVE), also called duck plague, is one of the major contagious and fatal diseases of ducks, geese and swan. It is caused by duck enteritis virus (DEV)/Anatid herpesvirus-1 of the genus Mardivirus, family Herpesviridae, and subfamily Alpha-herpesvirinae. Of note, DVE has worldwide distribution, wherein migratory waterfowl plays a crucial role in its transmission within and between continents. Furthermore, horizontal and/ or vertical transmission plays a significant role in disease spread through oral-fecal discharges. Either of sexes from varying age groups of ducks is vulnerable to DVE. The disease is characterized by sudden death, vascular damage and subsequent internal hemorrhage, lesions in lymphoid organs, digestive mucosal eruptions, severe diarrhea and degenerative lesions in parenchymatous organs. Huge economic losses are connected with acute nature of the disease, increased morbidity and mortality (5%-100%), condemnations of carcasses, decreased egg production and hatchability. Although clinical manifestations and histopathology can provide preliminary diagnosis, the confirmatory diagnosis involves virus isolation and detection using serological and molecular tests. For prophylaxis, both live-attenuated and killed vaccines are being used in broiler and breeder ducks above 2 weeks of age. Since DEV is capable of becoming latent as well as shed intermittently, recombinant subunit and DNA vaccines either alone or in combination (polyvalent) are being targeted for its benign prevention. This review describes DEV, epidemiology, transmission, the disease (DVE), pathogenesis, and advances in diagnosis, vaccination and antiviral agents/therapies along with appropriate prevention and control strategies.

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Section: Molecular Diagnosis Of Devmentioning

confidence: 99%

Duck virus enteritis (duck plague) – a comprehensive update

Dhama

Kumar

Saminathan

et al. 2017

Veterinary Quarterly

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“…The main isothermal methods include loop-mediated isothermal amplification (LAMP; Notomi et al, 2000 ), nucleic acid sequence-based amplification (NASBA; Mugasa et al, 2014 ), rolling circle amplification (RCA; Ali et al, 2014 ), helicase-dependent amplification (HDA; Barbieri et al, 2014 ), strand displacement amplification (SDA; Qiu et al, 2013 ), isothermal and chimeric primer-initiated amplification of nucleic acids (ICANs; Asiello and Baeumner, 2011 ), and signal-mediated amplification of RNA technology (SMART; Zanoli and Spoto, 2012 ). Among them, LAMP has been widely adopted and further developed for widespread clinical use (Lee et al, 2015 ; Nakano et al, 2015 ; Neeraja et al, 2015 ; Oriero et al, 2015 ), the diagnosis of infectious diseases (Mori and Notomi, 2009 ; Kinoshita et al, 2015 ; Mansour et al, 2015 ), rapid testing of food products (D'Agostino et al, 2015 ; Ferrara et al, 2015 ; Sun et al, 2015 ) and environmental samples (Niessen, 2015 ; Shi et al, 2015 ), and also for detecting exogenous genes in GMOs (Zhou et al, 2014 ; Liu et al, 2015 ; Feng et al, 2015 ; Huang et al, 2015 ; Singh et al, 2015 ; Wang et al, 2015 ; Zhang et al, 2016 ) in the past decade.…”

Section: Introductionmentioning

confidence: 99%

Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay

et al. 2016

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Genetic engineering offers an attractive alternative in sugarcane breeding for increasing cane and sugar yields as well as disease and insect resistance. Bar transgenic sugarcane employing the herbicide tolerance is a useful agronomical trait in weed control. In this study, a loop-mediated isothermal amplification (LAMP) assay for rapid detection of the bar gene in transgenic sugarcane has been developed and evaluated. A set of six primers was designed for LAMP-based amplification of the bar gene. The LAMP reaction conditions were optimized as follows: 5.25 mM of Mg2+, 6:1 ratio of inner vs. outer primer, and 6.0 U of Bst DNA polymerase in a reaction volume of 25.0 μL. The detection limit of the recombinant plasmid 1Ac0229 was as low as 10 copies in the developed LAMP, which was 10-fold higher sensitive than that of conventional PCR. In 100 putative transgenic lines, the bar gene was detected in 100/100 cases (100%) by LAMP and 97/100 cases (97%) by conventional PCR, respectively. In conclusion, the developed LAMP assay is visual, rapid, sensitive, reliable, and cost-effective for detection of the bar specific transgenic sugarcane.

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“…Many LAMP assays have been developed and validated for important epizootic diseases, including 18 viruses thought notifiable of ruminants, swine and poultry by the World Organization for Animal Health (OIE) (Mansour et al, 2015). The LAMP assay was also developed for detection of the Tembusu virus (Tang et al, 2016), four immunosuppressive viruses (Song et al, 2018), avian leucosis virus (Peng et al, 2015), avian reovirus (Kumar et al, 2017), and found to be useful also in the detection of MDV in feathers and internal organs of infected chickens (Woźniakowski and Samorek-Salamonowicz, 2014).…”

Section: The Loop-mediated Isothermal Amplificationmentioning

confidence: 99%

Biotic concerns in generating molecular diagnosis matrixes for 4 avian viruses with emphasis on Marek’s disease virus

Davidson¹

2019

Journal of Virological Methods

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A B S T R A C TThe great advance in the field of diagnosis of avian viruses is reflecting the highly sophisticated molecular assays of the human and general virology in providing highly sensitive and fast methods of diagnosis. The present review will discuss the biotic factors and the complexities that became evident with the evolution of the novel molecular diagnostic assays with emphasis on 4 avian viruses, chicken anemia, infectious laryngotracheitis, turkey meningoencephalitis, but mainly on Marek's disease virus.To create a biologically meaningful diagnosis, attention should be dedicated to various biotic factors and not only of the diagnostic assay. Included among the important factors are, (a) the sample examined and the sampling strategy, (b) the outcomes of the pathogen amplification ex vivo, (c) the sampling time and its reflection on the disease diagnosis, (d) the impact of simultaneous multiple virus-infections regarding the ability to demonstrate all pathogens and inter-and intra-interactions between the pathogens. A concerted consideration of the relevant factors and the use of advanced molecular diagnostic assay would yield biologically significant diagnosis in real-time that would beneficiate the poultry industry. Recently Shojaei et al. (2015) and Astill et al. (2018) described novel methodologies developed to detect rapidly avian viruses, such as

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Loop-mediated isothermal amplification for diagnosis of 18 World Organization for Animal Health (OIE) notifiable viral diseases of ruminants, swine and poultry

Cited by 47 publications

References 84 publications

Duck virus enteritis (duck plague) – a comprehensive update

Duck virus enteritis (duck plague) – a comprehensive update

Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay

Biotic concerns in generating molecular diagnosis matrixes for 4 avian viruses with emphasis on Marek’s disease virus

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