Loop-Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus

Pham, Hang; Nakajima, Chie; Ohashi, Kazuhiko; Onuma, Misao

doi:10.1128/jcm.43.4.1646-1650.2005

Cited by 111 publications

(70 citation statements)

References 24 publications

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“…However, this detection is limited when the turbidity of reaction is low. To increase the sensitivity, LAMP products stained with ethidium bromide were used [15]. Unfortunately, the product of detection system with ethidium bromide has several limitations, such as generation of hazardous waste and less sensitivity than that of SYBR Green.…”

Section: Introductionmentioning

confidence: 99%

Detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Wongsawat¹,

Dharaku²,

Narat³

et al. 2011

Health

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Classical swine fever virus (CSFV) is the causative agent of Classical swine fever which is a highly contagious disease affecting swine and resulting in severe economic losses. In this study, we developed reverse transcription loopmediated isothermal amplification (RT-LAMP) assay targeting the 5'UTR gene for the detection of CSFV. This amplification method can be obtained in 1 h under isothermal conditions (65°C) employing a set of six specific primers mixtures. Amplification product was visualized by using hydroxynaphthol blue (HNB) dye and agarose gel electrophoresis. The sensitivity was 100 copy numbers. No cross-reactivity related to Japanese encephalitis virus (JEV) and porcine reproductive and respiratory syndrome virus (PR-RSV) was demonstrated. The results demonstrated that the RT-LAMP assay is a useful tool for the rapid and sensitive for CSFV detection in swine.

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Section: Introductionmentioning

confidence: 99%

Detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Wongsawat¹,

Dharaku²,

Narat³

et al. 2011

Health

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“…Most molecular techniques involve a polymerase chain reaction (PCR) and, because NDV has an RNA genome, reverse transcription (RT) to produce a DNA copy of the genome, which is an essential initial step. Alternatively, a loopmediated isothermal amplification can be used instead of PCR for the detection of ND RNA (Pham et al 2005b). …”

Section: Nucleic Acid Detectionmentioning

confidence: 99%

Opinion of the Scientific Panel on Animal Health and Welfare (AHAW) to review Newcastle disease focussing on vaccination worldwide in order to determine its optimal use for disease control purposes

2007

EFS2

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SUMMARYIn view of a revision of the Community rules on the control of Newcastle disease (ND), laid down in Council Directive 92/66/EEC, a disease of major importance for poultry and other birds, the Commission requested EFSA to provide a complete and update review on this disease, concerning the definition, the role of pigeons and other avian species in the spread of ND and the vaccines used. A risk assessment approach was not requested however, in order to evaluate the present vaccination control strategies, an evaluation of the available data was required.The working group agreed that the scope of the report should consider the present scientific knowledge and analyse the available epidemiological data from Animal Disease Notification System ADSN system (DG SANCO), HANDISTATUS II data base (OIE) and data from the EU Reference Laboratory (Veterinary Laboratory Agency, VLA, Weybridge) taking into consideration the above mentioned mandate. The evaluation of vaccines and vaccination programmes was carried out in close cooperation with EMEA, who delegated two representatives to the working group.EFSA's scientific Panel on Animal Health and Welfare reviewed ND according to the mandate and drew the following major conclusions and recommendations:In the current EU Directive (92/66/EEC), Newcastle disease is defined as an infection of poultry caused by a virus of avian paramyxovirus type 1 (APMV-1) which has an intracerebral pathogenicity index (ICPI) in day-old chicks (Gallus gallus) of 0.7 or greater. The Panel has no scientific evidence to recommend a change on the definition of ND to include viruses with an ICPI below 0.7. ND should be defined as an infection of poultry and other captive birds with an APMV-1 meeting the criteria as described by the SCAHAW in 1998, allowing the sequencing of the cleavage site of the F gene as a supplement to the ICPI. The use of ICPI tests, for welfare reasons amongst others, should be restricted to situations where there is failure to demonstrate the presence of multiple basic amino acids at the Cterminus of the F2 protein and F (phenylalanine) at residue 117 of the F1 protein. It is recommended that for the purpose of a revised Directive for the control of ND the same definitions for poultry and captive birds as provided in the Directive 2005/94 for the control of AI is used. In addition the future Directive for ND should give a definition of racing pigeons.Biosecurity measures constitute the most important barrier in preventing the introduction, transmission and spread of ND. Prevention of secondary cases can be achieved through bio-containment. The major risk of ND spread from the index case is the movement of live poultry and birds and all activities linked to mechanical transfer of the virus by human beings, vehicles, crates and cages and any type of farm equipment.In addition to biosecurity measures, vaccination can be a valuable tool to control disease, however suboptimal use may result in the infection becoming endemic. Optimal vaccination programs are expected to prevent o...

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“…The technique uses DNA polymerase and four specially designed primers that are specific for sequences on the sense and antisense strands of the target DNA. Loopmediated isothermal amplification-based assays have been used to detect several viruses (222,234,235,279) and bacteria (86,130,140,194,257,265) and at least one fungus (85). Successful detection has been reported in tests involving several clinical samples (85,140,234,235,257,279).…”

Section: Nucleic Acid Detectionmentioning

confidence: 99%

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

et al. 2005

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SUMMARY Recent events have made public health officials acutely aware of the importance of rapidly and accurately detecting acts of bioterrorism. Because bioterrorism is difficult to predict or prevent, reliable platforms to rapidly detect and identify biothreat agents are important to minimize the spread of these agents and to protect the public health. These platforms must not only be sensitive and specific, but must also be able to accurately detect a variety of pathogens, including modified or previously uncharacterized agents, directly from complex sample matrices. Various commercial tests utilizing biochemical, immunological, nucleic acid, and bioluminescence procedures are currently available to identify biological threat agents. Newer tests have also been developed to identify such agents using aptamers, biochips, evanescent wave biosensors, cantilevers, living cells, and other innovative technologies. This review describes these current and developing technologies and considers challenges to rapid, accurate detection of biothreat agents. Although there is no ideal platform, many of these technologies have proved invaluable for the detection and identification of biothreat agents.

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Loop-Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus

Cited by 111 publications

References 24 publications

Detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Opinion of the Scientific Panel on Animal Health and Welfare (AHAW) to review Newcastle disease focussing on vaccination worldwide in order to determine its optimal use for disease control purposes

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

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