Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression

Sreejith, Kamalalayam Rajan; Umer, Muhammad; Singha, Pradip; Nguyen, Nhat‐Khuong; Kasetsirikul, Surasak; Ooi, Chin Hong; Shiddiky, Muhammad J. A.; Nguyen, Nam‐Trung

doi:10.3390/mi12080905

Cited by 6 publications

(6 citation statements)

References 34 publications

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“…The images were processed with ImageJ software to quantitatively evaluate the variation in fluorescent intensity with respect to time. The numerical equivalent values of the fluorescence were offset corrected and normalized using the equation [10,[19][20][21].…”

Section: Performance Of Portable Device For Lamp Based Detection Of Sars-cov-2mentioning

confidence: 99%

“…Using LAMP for an RNA virus like SARS-CoV-2 can further reduce the cost and analysis time by eliminating the need for RNA isolation and cDNA conversion steps. Additionally, many on-chip LAMP based diagnosis and pathogen detection were reported recently [10][11][12][13][14][15][16], which could provide better sample handling platform for LAMP based disease diagnosis and pathogen detection. Taken together, these features make a LAMP based portable/handheld device an excellent candidate for mass screening and point-of-care patient management in our time of widespread pandemic.…”

Section: Introductionmentioning

confidence: 99%

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A Portable Device for LAMP Based Detection of SARS-CoV-2

et al. 2021

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This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 102 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.

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Section: Performance Of Portable Device For Lamp Based Detection Of Sars-cov-2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Portable Device for LAMP Based Detection of SARS-CoV-2

et al. 2021

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“…The present paper reports the synthesis, characterisation, and active manipulation of liquid beads with a magnetic solid shell and a liquid core. Subsequently we demonstrate a two-dimensional (2D) self-assembly of magnetic liquid beads with potential nucleic acid amplification techniques such as polymerase chain reaction (PCR) (Sreejith et al 2020 ) and loop mediated isothermal amplification (LAMP) (Sreejith et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and active manipulation of magnetic liquid beads

Yadav,

Galogahi,

Vashi

et al. 2024

Biomed Microdevices

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We report the fabrication and characterisation of magnetic liquid beads with a solid magnetic shell and liquid core using microfluidic techniques. The liquid beads consist of a fluorinated oil core and a polymer shell with magnetite particles. The beads are generated in a flow-focusing polydimethylsiloxane (PDMS) device and cured by photo polymerisation. We investigated the response of the liquid beads to an external magnetic field by characterising their motion towards a permanent magnet. Magnetic sorting of liquid beads in a channel was achieved with 90% efficiency. The results show that the liquid beads can be controlled magnetically and have potential applications in digital microfluidics including nucleic acid amplification, drug delivery, cell culture, sensing, and tissue engineering. The present paper also discusses the magnetophoretic behaviour of the liquid bead by varying its mass and magnetite concentration in the shell. We also demonstrated the two-dimensional self-assembly of magnetic liquid beads for potential use in digital polymerase chain reaction and digital loop mediated isothermal amplification. Graphical abstract

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“…[20][21][22] Recently, liquid marbles were used as microreactors for chemical and biological applications owing to their superior characteristics. [23][24][25][26][27][28][29][30][31] First, liquid marbles can conveniently accommodate a wide range of volumes from sub-microlitres to a few hundred microliters. In addition, liquid marbles can be produced in a facile manner by rolling small droplets over a powder bed.…”

Section: Introductionmentioning

confidence: 99%