Loop-mediated isothermal amplification (LAMP) detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758) in China

A Historical Overview of Research on Babesia orientalis, a Protozoan Parasite Infecting Water Buffalo

“…The counties north of Yangtze River side, including Macheng, Xiaogan and Hongan of Hubei province were B. orientalis free (Liu et al, 2007). However, in a later study, 1 (1/88) and 2 (2/88) positive cases were reported north of Yangtze River by semi-nest PCR and a loop-mediated isothermal amplification assay (LAMP), respectively (He et al, 2009b). The finding means B. orientalis had been spread to non-epidemic areas ( Figure 2 ), possibly by convenient transportations of water buffalo from south to north because more and more bridges built on the river make the river no longer a natural barrier.…”

Occurrence of Theileria and Babesia species in water buffalo (Bubalus babalis, Linnaeus, 1758) in the Hubei province, South China

“…It has recently been shown to have spread to the north of the Yangtse River, posing a serious threat to the water buffalo industry (He et al, 2009).…”

Identification and characterization of a novel 34 kDa merozoite protein in Babesia orientalis

“…These buffalo were splenectomised 2 weeks before experimental B. orientalis infection. Each buffalo was subcutaneously injected with 4 ml of blood containing B. orientalis (Wuhan strain, 1% parasitaemia) (He et al, 2009b). Blood samples were collected every day to monitor parasitaemia until it reached 3%.…”

“…It is necessary to develop a specific and sensitive diagnostic method. Many useful diagnostic methods for B. orientalis have been developed recently, which include semi-nested PCR , reverse line blot hybridization assay (RLB) (He et al, 2012), loop-mediated isothermal amplification (LAMP) (He et al, 2009b) and real-time PCR (He et al, 2011). However, most of these methods are time consuming, and require specialized laboratory equipments and trained personnel.…”