2020
DOI: 10.1016/j.jiac.2020.03.019
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Loop-mediated isothermal amplification (LAMP) in the respiratory specimens for the diagnosis of pediatric pulmonary tuberculosis: A pilot study

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Cited by 11 publications
(7 citation statements)
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“…In our study, the combination of pathology with TB-LAMP showed that TB-LAMP was a powerful tool for detecting MTB infection in patients with stage II or III TB empyema, presenting a sensitivity of 84.7%, NPV of 67.3%, a specificity of 96.0%, and PPV of 98.5% when CCRS was used as a reference. This is in line with previous research carried out by Sreedeep et al, who concluded that the combination of TB-LAMP and culture would increase the sensitivity significantly according to CCRS ( 21 ). In our study, the performance of combining pathology and TB-LAMP was also significantly better than that of any single test for TB empyema.…”
Section: Discussionsupporting
confidence: 92%
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“…In our study, the combination of pathology with TB-LAMP showed that TB-LAMP was a powerful tool for detecting MTB infection in patients with stage II or III TB empyema, presenting a sensitivity of 84.7%, NPV of 67.3%, a specificity of 96.0%, and PPV of 98.5% when CCRS was used as a reference. This is in line with previous research carried out by Sreedeep et al, who concluded that the combination of TB-LAMP and culture would increase the sensitivity significantly according to CCRS ( 21 ). In our study, the performance of combining pathology and TB-LAMP was also significantly better than that of any single test for TB empyema.…”
Section: Discussionsupporting
confidence: 92%
“…It was demonstrated that TB-LAMP had moderate sensitivity (pooled sensitivity 77.7%, 95% CI 71.2–83.0%) and high specificity (98.1%, 95% CI 95.7–99.2%) when using the most stringent culture-based reference standard, therefore, has the potential to be a useful diagnostic test for pulmonary TB ( 20 ). The improved sensitivity similar specificity of our study was also in line with a previous study using TB-LAMP for extrapulmonary TB (EPTB) diagnosis (sensitivity and specificity of LAMP against culture was 85.71 and 88.89%; against CRS was 80 and 88.6% respectively) ( 21 ). In our study, the combination of pathology with TB-LAMP showed that TB-LAMP was a powerful tool for detecting MTB infection in patients with stage II or III TB empyema, presenting a sensitivity of 84.7%, NPV of 67.3%, a specificity of 96.0%, and PPV of 98.5% when CCRS was used as a reference.…”
Section: Discussionsupporting
confidence: 91%
“…The specificity of TB-LAMP was high (99.1%, 109/110). Similar findings have been reported by Sreedeep et al 24 in a recently published cross-sectional analytical study using respiratory specimens (sputum, gastric lavage, bronchoalveolar lavage and/or endotracheal aspirates) in children with pulmonary tuberculosis. TB-LAMP conducted using BALF samples can provide faster results, allowing an early and accurate TB diagnosis in both bacteriologically confirmed and clinically diagnosed TB children.…”
Section: Discussionsupporting
confidence: 90%
“…Previously, many studies have confirmed that using the IS6110 sequence (several copies exist in the MTBC genome) as a target for the LAMP reaction obtained the preferable sensitivity and specificity for the detection of typical MTBC strains in the diagnosis of TB (Singh et al, 2004;Joon et al, 2017;Sreedeep et al, 2020). Recently, a diagnostic method has achieved rapid detection of MTBC based on mLAMP combined with singlelabeled LFB but could not distinguish MTB from other members of MTBC due to the limitations of gene selection and LFB design (Wang et al, 2021).…”
Section: Discussionmentioning
confidence: 99%