Loop-Mediated Isothermal Amplification (Lamp): Sensitive and Rapid Detection of Schistosoma Haematobium Dna in Urine Samples of Egyptian Suspected Cases.

Bayoumi, A El; Alrefai, Sherif; Badr, Mohamed; El-Aal, Asmaa Ahemed Abd; Akkad, Dina M.H. El; Saad, Naglaa; Elesaily, Khalid; Aziz, Inas Z. Abdel

doi:10.21608/jesp.2016.88677

Cited by 6 publications

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“…S. haematobium causes urogenital pathologies such as dysuria and hematuria and so called female genital schistosomiasis (FGS) if eggs are deposited in the female genitalia [6]. FGS impairs fertility, e.g., ectopic pregnancy and miscarriage, and susceptibility to viruses, e.g., human immunodeficiency virus [7] and papillomavirus, and progressing to malignancies augmented by calcification, e.g., squamous cell carcinomas and sandy patches [8][9][10][11][12]. Ectopic excess egg deposition or erroneous migrating adult worms within the central nervous Acute schistosomiasis or Katayama fever seen among individuals without past parasitic exposure presents as debilitating febrile illness coupled with, e.g., headache, myalgia, fatigue, diarrheal, as well as respiratory symptoms, and hepato-and/or splenomegaly following an incubation period of up to 10-12 weeks.…”

Section: Introductionmentioning

confidence: 99%

Clinical Applications of Isothermal Diagnosis for Human Schistosomiasis

Panzner

2022

Encyclopedia

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About 250 million people affected, 779 million people at risk of infection, and 440 million people with residual morbidity are globally attributable to schistosomiasis. Highly sensitive and specific, simple, and fast to perform diagnostics are required for detecting trace infections, and applications in resource-poor settings and large-scale assessments. Research assessing isothermal diagnoses of S. japonicum, S. haematobium, S. mansoni, mixed infections, and schistosomal hybrids among clinical human specimens was investigated. Loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA) and combined techniques were identified. Both LAMP and RPA reached species-dependent 100% sensitivity, and detection levels within femtogram and nanogram amounts for pure and hybridale breeds. Cross-reactivity among Schistosoma species and co-endemic pathogens was rare, though research on diagnostic markers and primer optimization should continue. Operating with ready-to-use lyophilized reagents, simplified and inexpensive nucleic acid extraction, tolerability to likely inhibitors, and enzyme stability at ambient temperature is advantageous. RPA performed optimal at 35–39 °C within 5–10 min. while LAMP operated at 61–65 °C for up to 120 min.; properties are preferable over assays requiring expensive laboratory equipment. DNA degradation could be prevented by stabilizing substances. A limitation throughout warranting future research is the small sample size reaching a few hundred participants at the maximum. Isothermal diagnostics are highly valuable in detecting trace infections seen subsequent to chemotherapeutic treatment, and among apparently healthy individuals, both constituting likely sources of ongoing pathogen transmission. Its expansion to the vaccine field for assessing parasitological trial endpoints could be considered.

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Section: Introductionmentioning

confidence: 99%

Clinical Applications of Isothermal Diagnosis for Human Schistosomiasis

Panzner

2022

Encyclopedia

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“…The overall prevalence by LAMP was significantly higher than microscopy when testing both purified DNA (73.8% vs. 50.6%) and crude urine (63.4% vs. 50.6%) samples, respectively [ 19 ]. Bayoumi et al [ 27 ] also evaluated a LAMP test to detect S. haematobium DNA in urine samples collected from patients suspected of urogenital schistosomiasis attending an outpatient clinic in Egypt. LAMP resulted in a 100% sensitivity and 63.16% specificity when compared with conventional urine filtration followed by microscopy for eggs detection.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Loop-Mediated Isothermal Amplification (LAMP) in Urine Samples for the Diagnosis of Imported Schistosomiasis

Salas-Coronas,

Luzón-García,

Crego-Vicente

et al. 2023

TropicalMed

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Migratory flows and international travel are triggering an increase in imported cases of schistosomiasis in non-endemic countries. The present study aims to evaluate the effectiveness of the LAMP technique on patients’ urine samples for the diagnosis of imported schistosomiasis in a non-endemic area in comparison to a commercial immunochromatographic test and microscopic examination of feces and urine. A prospective observational study was conducted in sub-Saharan migrants attending the Tropical Medicine Unit, Almería, Spain. For schistosomiasis diagnosis, serum samples were tested using an immunochromatographic test (Schistosoma ICT IgG-IgM). Stool and urine samples were examined by microcopy. Urine samples were evaluated by combining three LAMP assays for the specific detection of Schistosoma mansoni, S. haematobium, and for the genus Schistosoma. To evaluate the diagnostic accuracy, a latent class analysis (LCA) was performed. In total, 115 patients were included (92.2% male; median age: 28.3 years). Of these, 21 patients (18.3%) were diagnosed with schistosomiasis confirmed by microscopy, with S. haematobium being the most frequent species identified (18/115; 15.7%). The Schistosoma ICT IgG-IgM test result was 100% positive and Schistosoma-LAMP was 61.9% positive, reaching as high as 72.2% for S. haematobium. The sensitivity and specificity estimated by LCA, respectively, were: 92% and 76% for Schistosoma ICT IgG-IgM, 68% and 44% for Schistosoma-LAMP, and 46% and 97% for microscopy. In conclusion, the Schistosoma-LAMP technique presented a higher sensitivity than microscopy for the diagnosis of imported urinary schistosomiasis, which could improve the diagnosis of active infection, both in referral centers and in centers with limited experience or scarce resources and infrastructure.

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“…Urogenital pathologies manifest as dysuria, hematuria, and female genital schistosomiasis [54]. The latter impairs susceptibility to predominantly viral pathogens [55], and fertility, e.g., ectopic pregnancy and miscarriage, in addition to progression to malignancies, e.g., squamous cell carcinomas and sandy patches [44,[56][57][58][59]. Notably, ectopic excess egg retention or erroneous worm migration in the central nervous system induces cognitive and physical impairments [60] that are seen in endemic settings [9,48,61].…”

Section: Epidemiology Transmission and Pathogenicitymentioning

confidence: 99%

Transmission Modelling for Human Non-Zoonotic Schistosomiasis Incorporating Vaccination: Guiding Decision- and Policymaking

Panzner

2024

Parasitologia

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Schistosomiasis, acquired by skin-penetrating cercariae of dioecious digenean schistosomes during freshwater contact, afflicts nearly 260 and 440 million people with active infections and residual morbidity, respectively. About 10 million women at reproductive age contract schistosomiasis during gestation every year. Acute schistosomiasis is characterized by pre-patent pro-inflammatory CD4+ T-helper 1 or CD4+ Th1/T-helper 17 reactivity against immature schistosomulae. Chronic schistosomiasis is dominated by post-patent anti-inflammatory CD4+ T-helper 2 reactivity against ova epitopes. Flukes co-exist in immunocompetent definitive hosts as they are capable of evading their defense mechanisms. Preventive measures should be complemented by vaccination, inducing long-term protection against transmission, infection, and disease recurrence, given the latest advancements in schistosomal vaccines. Vaccines become pivotal when considering constraints of chemotherapy, i.e., lack of protection against re-infection, and evolving resistance or reduced sensitivity. Transmission models for human non-zoonotic schistosomiasis incorporating vaccination available in PubMed, Embase and Web of Science up to 31 December 2023 are presented. Besides conceptual model differences, predictions meant to guide decision- and policymaking reveal continued worm harboring that facilitates transmission besides residual infections. In addition, increased susceptibility to re-infection and rebound morbidity, both shifted to later life stages following the intervention, are forecasted. Consequently, a vaccination schedule is pivotal that considers the optimal age for initial immunization, i.e., pre-schoolchildren or schoolchildren in a cohort-based or population-based manner, while incorporating potential non-adherers promoting ongoing transmission. Longevity over magnitude of vaccine protection to antigenic schistosomal moieties is crucial. Accounting for pre-acquired immunity from natural exposure, in utero priming in addition to herd immunity, and induced by chemotherapy is crucial. Combining, as a multi-component approach, long-term effects of vaccination with short-term effects of chemotherapy as regular repeated vaccine-linked therapy seems most promising to achieve WHO’s endpoints of transmission elimination and morbidity control.

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Loop-Mediated Isothermal Amplification (Lamp): Sensitive and Rapid Detection of Schistosoma Haematobium Dna in Urine Samples of Egyptian Suspected Cases.

Cited by 6 publications

References 0 publications

Clinical Applications of Isothermal Diagnosis for Human Schistosomiasis

Clinical Applications of Isothermal Diagnosis for Human Schistosomiasis

Evaluation of Loop-Mediated Isothermal Amplification (LAMP) in Urine Samples for the Diagnosis of Imported Schistosomiasis

Transmission Modelling for Human Non-Zoonotic Schistosomiasis Incorporating Vaccination: Guiding Decision- and Policymaking

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