Loop-mediated isothermal amplification (LAMP) test for specific and rapid detection of<i>Brucella abortus</i>in cattle

Karthik, Kumaragurubaran; Rathore, Rajesh; Thomas, Prasad; Arun, T.R.; Viswas, K.N.; Agarwal, R. K.; Manjunathachar, H V; Dhama, Kuldeep

doi:10.1080/01652176.2014.966172

Cited by 33 publications

(40 citation statements)

References 27 publications

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“…(Karthik et al, 2014b). LAMP assay for specific diagnosis of B. abortus was also developed which was 100 fold more sensitive than the commonly employed PCR (Karthik et al, 2014c). Severity of bone damage in cases of poll evil and fistulous withers can be identified by radiography.…”

Section: Serological Tests Like Rose Bengal Plate Test (Rbpt) Standamentioning

confidence: 99%

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Karthik¹,

Prabakar²,

Bharathi³

et al. 2016

JEBAS

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KEYWORDS Brucellosis Equine B. abortus Poll evil Fistulous withers LAMP ABSTRACTBrucellosis is one of the major zoonotic diseases that affect several domestic animals, wild animals and also marine mammals. Though there is no specific Brucella sp. that can affect horses, B. abortus and B. suis can affect horses naturally and B. canis experimental infection has also been reported in equines. Brucellosis in equines is characterized by two conditions namely Poll evil and fistulous withers. Organism has its predilection for joints, ligaments and tendons in case of equines and causes inflammatory conditions leading to formation of fistula. Equine brucellosis has been documented from several parts of the world and prevalence has been reported time to time mostly based on serological diagnosis. Diagnosis of brucellosis mainly depends on serological methods though isolation of the organism is the gold standard. Due to hazardous nature of the pathogen, tests like Rose Bengal plate agglutination test, Standard tube agglutination test and other serological assays are commonly employed. Isothermal amplification assay like LAMP are gaining momentum these years due to swiftness in diagnosis of the pathogen. LAMP with high specificity and sensitivity for detection of Brucella spp. and also B. abortus has been developed in the recent years. Prevention and control of brucellosis is of utmost important to halt the spread of the organism to other animals and human. Trauma is a major reason for predisposition of poll evil and fistulous withers hence proper fitting of saddle will help to prevent the disease. Housing and feeding the horses separately can prevent spread of disease from cattle. The present review discusses equine brucellosis, its epidemiology, pathogenesis, clinical signs along with appropriate prevention and control strategies to be adapted.

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Section: Serological Tests Like Rose Bengal Plate Test (Rbpt) Standamentioning

confidence: 99%

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Karthik¹,

Prabakar²,

Bharathi³

et al. 2016

JEBAS

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“…; Karthik et al . ). The advantage of LAMP, in comparison to PCR‐based methods, is its low susceptibility to inhibition by contaminants present in DNA or RNA samples.…”

Section: Discussionmentioning

confidence: 97%

Cross-priming amplification for detection of bovine viral diarrhoea virus species 1 and 2

2015

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“…Patel et al, (2015) and Karthik et al, (2014) used species specific +IS711 primers for detection of Brucella abortus and they yielding 498bp band and Vizcaino et al, (1996)used species specific omp31 primers for detection of B. melitensis and they yielding 723bp band when electrophoresed through 2% agarose gel. Lopez Goni et al, (2008) reported Bruce-ladder was species specific and all the strains and biovars from the same Brucella species gave the same profile.…”

Section: Molecular Detection Of Brucella From Clinical Samplesmentioning

confidence: 99%

Clinico-Seroepidemiology and Molecular Characterization of Brucellosis in Animals

Chauhan¹,

Patel²,

Kala³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Loop-mediated isothermal amplification (LAMP) test for specific and rapid detection ofBrucella abortusin cattle

Cited by 33 publications

References 27 publications

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Cross-priming amplification for detection of bovine viral diarrhoea virus species 1 and 2

Clinico-Seroepidemiology and Molecular Characterization of Brucellosis in Animals

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