Low cost, disposable biosensors allow detection of antibodies with results equivalent to ELISA in 15min

Cork, Jennifer; Jones, Rosemary; Sawyer, Jason

doi:10.1016/j.jim.2012.10.007

Cited by 20 publications

(11 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Biosensors are a promising alternative to expensive conventional medical diagnostic techniques commonly employed in clinical laboratories such as enzyme-linked immunosorbent assays (ELISA) or Western blots. − Compared to such techniques, biosensors have the advantage of being cost effective, quick, and portable and therefore suitable for point-of-care testing (POCT). − Although some specific biosensors are already present in the market, including, for example, glucose monitoring, − they are not widely employed yet to screen biomarkers for early diagnosis of diseases . Of all classes of sensing strategies, optical biosensors are a promising choice for early diagnostics, as they have the potential for low cost, rapid response, and high reliability. ,,− Furthermore, significant research effort is currently focused on developing multiplexed architectures to increase the number of biomarkers that could be screened simultaneously to improve the confidence interval of the analysis. − However, for current clinical techniques such as ELISA, a major cost lies in the quantity of reagents, particularly antibodies, required to functionalize the surface. , Although significant focus is given in the literature on ways of improving biosensor performance, little attention has been paid to date to creating sensing architectures that limit the quantity of reagents required for analysis without loss in sensitivity or specificity.…”

Section: Introductionmentioning

confidence: 99%

“…16−20 However, for current clinical techniques such as ELISA, a major cost lies in the quantity of reagents, particularly antibodies, required to functionalize the surface. 21,22 Although significant focus is given in the literature on ways of improving biosensor performance, little attention has been paid to date to creating sensing architectures that limit the quantity of reagents required for analysis without loss in sensitivity or specificity.…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dip Biosensor Based on Localized Surface Plasmon Resonance at the Tip of an Optical Fiber

Sciacca

Monro

2014

Langmuir

View full text Add to dashboard Cite

A dip biosensor is realized by depositing metallic nanoparticles onto the tip of a cleaved optical fiber. Light coupled into the fiber interacts with the localized surface plasmons within the nanoparticles at the tip; a portion of the scattered light recouples into the optical fiber and is analyzed by a spectrometer. Characterization of the sensor demonstrates an inverse relationship between the sensitivity and the number of particles deposited onto the surface, with smaller quantities leading to greater sensitivity. The results obtained showed also that by depositing nanoparticles with distinct localized surface plasmon resonance signatures with limited overlap, as for the case of gold and silver nanospheres, a multiplexed dip biosensor can be realized by simply functionalizing the different nanoparticles with different antibodies after the fashion of an immunoassay. In this way different localized surface plasmons resonance bands responsive to different target analytes can be separately monitored, as further presented below, requiring a minimal quantity of reagents both for the functionalization process and for the sample analysis.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Dip Biosensor Based on Localized Surface Plasmon Resonance at the Tip of an Optical Fiber

Sciacca

Monro

2014

Langmuir

View full text Add to dashboard Cite

show abstract

“…While the enzyme-linked immunosorbent assay (ELISA) approach is a popular and precise technique widely applied for immunodetection of antigen molecules in diagnostic applications, immunosensors with MNP amplification can be potentially substituted for conventional ELISA kits, offering same or even better sensitivity, lower detection time, and minimal washing steps [77, 78]. Jeong et al [79] developed a highly sensitive electrochemical immunosensor for determination of the carcinoembryonic antigen (CEA) cancer biomarker through immobilizing the first antibody and a mediator (thionine, Th) on a AuNP-encapsulated dendrimer (Den/AuNP) and immobilizing the second antibody on MWCNTs, which were in turn conjugated with two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) as electrochemical labels.…”

Section: The Functions Of Mnps In Various Biosensor Typesmentioning

confidence: 99%

Noble metal nanoparticles in biosensors: recent studies and applications

Malekzad

Zangabad

Mirshekari

et al. 2017

Nanotechnology Reviews

305

119

View full text Add to dashboard Cite

The aim of this review is to cover advances in noble metal nanoparticle (MNP)-based biosensors and to outline the principles and main functions of MNPs in different classes of biosensors according to the transduction methods employed. The important biorecognition elements are enzymes, antibodies, aptamers, DNA sequences, and whole cells. The main readouts are electrochemical (amperometric and voltametric), optical (surface plasmon resonance, colorimetric, chemiluminescence, photoelectrochemical, etc.) and piezoelectric. MNPs have received attention for applications in biosensing due to their fascinating properties. These properties include a large surface area that enhances biorecognizers and receptor immobilization, good ability for reaction catalysis and electron transfer, and good biocompatibility. MNPs can be used alone and in combination with other classes of nanostructures. MNP-based sensors can lead to significant signal amplification, higher sensitivity, and great improvements in the detection and quantification of biomolecules and different ions. Some recent examples of biomolecular sensors using MNPs are given, and the effects of structure, shape, and other physical properties of noble MNPs and nanohybrids in biosensor performance are discussed.

show abstract

“…Além disso, a diversidade de alimentos disponíveis, a adição de nutrientes, a adulteração acidental ou deliberada e a demanda por produtos mais saudáveis por parte do consumidor contribuem para o desenvolvimento das pesquisas em biossensores. 142 Novas tecnologias específicas como biossensores acoplados a celulares 144 44,148,149 , microeletrodos e eletrodos descartáveis 55,96,150 foram desenvolvidas para esse mercado nos últimos anos e são capazes de fornecer informações específicas sobre possíveis contaminações como defensivos agrícolas, metais pesados, poluentes e compostos tóxicos, além da qualidade do produto final. [151][152][153]…”

Section: Munclassified

Biosensor and Beverages - Review

2016

View full text Add to dashboard Cite

In the beverages industry the challenge is to eliminate chemical and biological contaminants such as pesticides, antibiotics, and several microorganisms that may be present in food. The use of new legislations by responsible agencies generates an increase in the quality control of industrial products being evaluated through periodic chemical and microbiological analysis, as beverages. Thus, this review aimed to evaluate the application of biosensors in beverages samples and the progress of analytical methodology nowadays. An interesting alternative for carrying out these analyzes is the use of biosensors, because the advantages of these over conventional techniques are not only sensitive and selective, but also dispense a pre-treatment of samples, providing speed in analysis, minimum reagents cost and thus, reducing the financial cost. Biosensors are devices that combine the selectivity of a biological recognition element with the sensitivity of a transducer. The main objective is to produce a measurable signal proportional to the concentration of a specific compound or a group compounds which have interacted with the biological component. Despite the enormous research diversity involving biosensors for the industry, the application in the beverages for any compound is still restricted.

show abstract

Low cost, disposable biosensors allow detection of antibodies with results equivalent to ELISA in 15min

Cited by 20 publications

References 12 publications

Dip Biosensor Based on Localized Surface Plasmon Resonance at the Tip of an Optical Fiber

Dip Biosensor Based on Localized Surface Plasmon Resonance at the Tip of an Optical Fiber

Noble metal nanoparticles in biosensors: recent studies and applications

Biosensor and Beverages - Review

Contact Info

Product

Resources

About