Low-cost gelling agents for tissue culture propagation of plantain

Hegele, Suputtra; Hegele, M.; Wunsche, J.

doi:10.17660/actahortic.2021.1327.46

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…It was expensive and overexploited when agar was predominantly used as the gelling agent for microbial and plant cell culture media (Das et al 2019). Others have voiced concerns regarding agar applicability, especially for its limited resources overuse, and considerations include exorbitant pricing (Hassan and Moubarak 2020;Hegele et al 2021). Previous studies have investigated the use of Vol:.…”

Section: Mechanical Strengthmentioning

confidence: 99%

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

et al. 2022

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Bacterial cellulose (BC) is a natural biodegradable, eco-friendly fiber, lying within the nanoscale range. It is reputable for its various physical and chemical qualities, like high hydrophilicity, immense crystallinity, ease of sterility, being toxin-free, and extremely pure. Adding to its wide applicability in different fields, this study evaluated the applicability of a developed gelling substitute for plant tissue culture media. The BC matrix was characterized under the acronym PLATIBACGEL (PLAnt TIssue Culture BActerial Cellulose GEL), formed by Komagataeibacter hansenii AS.5, preisolated from rotten apple waste. Scanning electron microscope, Fourier-transform infrared, X-ray diffractometer, and tensile strength analyses confirmed the formation of purified, porous, and heterogeneous densely packed multiple network polymers possessing cellulose properties. The water holding capacity (WHC) values of wet and dried BC membranes were 9179% and 226.9%, respectively, and the water absorption rate (WAR) of dry BC membranes was higher than that of wet membranes. Using BC as a tissue culture gelling agent, six genotypes from tomato and wheat seeds were cultured in vitro, for guaranteeing explant genetic diversity, over seven treatments. Treatment 5, included PLATIBACGEL as the main constituent, improved and sustained all in vitro seed germination, root penetration, and plant support. Likewise, repeated tomato micropropagation subcultures were successful. Results demonstrated applying PLATIBACGEL as a promising, reusable, cheap, and reliable alternative plant micropropagation media gelling agent. Wherefore, plant cellular developers and tissue-culturists can utilize bio-polymers like BC for better understanding plant cell response to different in vitro culturing conditions, with expected beneficial returns on gelling agents industry and markets as well. Graphical abstract

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Section: Mechanical Strengthmentioning

confidence: 99%

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

et al. 2022

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“…Gelling agents used to solidify plant growth medium can contain many mineral nutrients reported to affect plant growth (Joshi, 2009). Generally, many studies have shown that the type of gelling agent used can influence tissues growth in vitro (Quiala et al, 2014;Ramassamy, 2014, 2015;Al-Mayahi, 2015;Raina, 2017;Hegele et al, 2021, therefore the study on selecting commercial grades of gelling agents is very important. There are limited studies about gelling agent effect routinely used in micropropagation on palm plant growth and development (Al-Mayahi, 2021;.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Gelling Agent Alternatives during in Vitro Multiplication of Yucca elephantipes L.

Mohamed Abass,

Thabet,

Abdelfattah

et al. 2023

Journal of Plant Production

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In vitro hardening materials like Agar and Phytagel are increasing in price, therefore cheaper gelling agents were needed. In this study, potato starch (Solanum tuberosum) and corn starch (Zea mays L.) at different rates (40, 50, 60, and 70 g/l) each alone or combined with agar at 2 or 4 g/l as well as 8 g agar/l as control were tested during in vitro multiplication of yucca (Yucca elephantipes L.). However, the first experiment, shoot proliferation percentage was 100% with all treatments (potato starch + agar) under study, media status was solid with any combination between potato and agar except that of potato alone was semi solid status. On the other hand, MS medium supplemented with potato alone at 70 g/l produced the most leaves per shoot and shoot length, while MS medium supplemented with agar at 4 g/l + potato at 40 g/l produced the greatest shoots per explant, with significant differences from the other treatments. For the second experiment, shoot proliferation percentage was 100% as well as media status was solid with all treatments (corn starch + agar) under study. MS medium supplemented with corn alone at 70 g/l provided the highest leaf number per shoot and shoot length, while MS medium supplemented with agar at 2 g/l + corn at 40 g/l produced the most shoots per explant with significant differences from the other treatments. Consequently, the present work suggests a low-cost alternative gelling agent that will reduce plant micro propagation production costs.

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Temporary Immersion Systems in Plant Micropropagation

Ramírez-Mosqueda,

Cruz-Cruz

2024

Methods in Molecular Biology

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Low-cost gelling agents for tissue culture propagation of plantain

Cited by 4 publications

References 16 publications

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

Evaluation of Gelling Agent Alternatives during in Vitro Multiplication of Yucca elephantipes L.

Temporary Immersion Systems in Plant Micropropagation

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