Low-cost, open-source 3D printed antibody dispenser for development and small-scale production of lateral flow assay strips

Han, Won Seok; Shin, Joong Ho

doi:10.1016/j.ohx.2021.e00188

Cited by 12 publications

(14 citation statements)

References 24 publications

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“…However, in the future as we shift focus to increasing detection sensitivity, we will analyze color intensity at test lines/dots. Nevertheless, few quantitative LFD biosensors [ 68 ] and low-cost 3D-printed antibody dispenser developments and small-scale production of lateral flow assays strips have been reported recently [ 69 ]. Further improvement including the evaluation of parameters important for on-field application, such as humidity and temperature with respect to stability of the immuno-dipstick, are ongoing and we hope to report the findings in the near future.…”

Section: Discussionmentioning

confidence: 99%

Immuno-Dipstick for Colletotrichum gloeosporioides Detection: Towards On-Farm Application

2022

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Early and quick detection of pathogens are crucial for managing the spread of infections in the biomedical, biosafety, food, and agricultural fields. While molecular diagnostics can offer the specificity and reliability in acute infectious diseases, detection of pathogens is often slowed down by the current benchtop molecular diagnoses, which are time consuming, labor intensive, and lack the mobility for application at the point-of-need. In this work, we developed a complete on-farm use detection protocol for the plant-devastating anthracnose agent: Colletotrichum gloeosporioides. Our methods combined a simplified DNA extraction on paper that is compatible with loop-mediated isothermal amplification (LAMP), coupled with paper-based immunoassay lateral flow sensing. Our results offer simple, quick, easy, and a minimally instrumented toolkit for Colletotrichum gloeosporioides detection. This scalable and adaptable platform is a valuable alternative to traditional sensing systems towards on-the-go pathogen detection in food and agriculture, biomedical, and other fields.

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Section: Discussionmentioning

confidence: 99%

Immuno-Dipstick for Colletotrichum gloeosporioides Detection: Towards On-Farm Application

2022

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“…There are two main types of detection zones, which are the spot and band types which demonstrated in the lower section band or spot-shape. However, the band-shape zones require special equipment to evenly transfer the designated solution onto the desired area [48]. The in-house reagent dispensing machine can be used with proper validation [48][49][50].…”

Section: Competitive Formatmentioning

confidence: 99%

“…However, the band-shape zones require special equipment to evenly transfer the designated solution onto the desired area [48]. The in-house reagent dispensing machine can be used with proper validation [48][49][50]. Dissimilar to the spot shape zones, simple biotechnology instruments, such as the pipette, can be used where access to the lateral flow dispenser is limited.…”

Section: Competitive Formatmentioning

confidence: 99%

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

et al. 2022

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Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a complicated matrix is challenging. The lateral flow immunoassay (LFA) is one of the immunoassays well-known for its simplicity, portability, and rapidity. In this review, the general principle, components, format, and application of the LFA for phytoproducts are discussed.

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“…However, the production cost of the techniques varies according to available medical resources. The minimum commercial cost of the available kits is recorded at $29.63, and the highest is $150 and $140 for antibody testing [ 222 ]. In Figure 6 A, according to the FIND database, 1570 total testing kits have been designed, out of which 639 are molecular assays-based and 925 are immunoassay-based testing.…”

Section: An Overview Of the Commercially Available Kitsmentioning

confidence: 99%

Advancements in Testing Strategies for COVID-19

et al. 2022

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The SARS-CoV-2 coronavirus, also known as the disease-causing agent for COVID-19, is a virulent pathogen that may infect people and certain animals. The global spread of COVID-19 and its emerging variation necessitates the development of rapid, reliable, simple, and low-cost diagnostic tools. Many methodologies and devices have been developed for the highly sensitive, selective, cost-effective, and rapid diagnosis of COVID-19. This review organizes the diagnosis platforms into four groups: imaging, molecular-based detection, serological testing, and biosensors. Each platform’s principle, advancement, utilization, and challenges for monitoring SARS-CoV-2 are discussed in detail. In addition, an overview of the impact of variants on detection, commercially available kits, and readout signal analysis has been presented. This review will expand our understanding of developing advanced diagnostic approaches to evolve into susceptible, precise, and reproducible technologies to combat any future outbreak.

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Low-cost, open-source 3D printed antibody dispenser for development and small-scale production of lateral flow assay strips

Cited by 12 publications

References 24 publications

Immuno-Dipstick for Colletotrichum gloeosporioides Detection: Towards On-Farm Application

Immuno-Dipstick for Colletotrichum gloeosporioides Detection: Towards On-Farm Application

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Advancements in Testing Strategies for COVID-19

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