Low energy (1–19 eV) electron scattering from condensed thymidine (dT) II: comparison of vibrational excitation cross sections with those of tetrahydrofuran and the recalibrated values of thymine

Lemelin, V.; Bass, A. D.; Cloutier, Pierre; Sanche, Léon

doi:10.1039/c9cp03448j

Cited by 2 publications

(1 citation statement)

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“…A similar observation was made recently for absolute vibrational excitation cross sections. The cross section values of thymidine do not correspond to the sum of the cross sections of its constituents [29]. To further elucidate the discrepancy in the SB cross sections, further experiments are required using e.g., double-stranded DNA, which has higher conformational stability over a longer range of strand lengths.…”

Section: Oligonucleotide Length Dependence Of Strand Break Cross Sectmentioning

confidence: 99%

Length and Energy Dependence of Low-Energy Electron-Induced Strand Breaks in Poly(A) DNA

Ebel

Bald

2019

IJMS

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The DNA in living cells can be effectively damaged by high-energy radiation, which can lead to cell death. Through the ionization of water molecules, highly reactive secondary species such as low-energy electrons (LEEs) with the most probable energy around 10 eV are generated, which are able to induce DNA strand breaks via dissociative electron attachment. Absolute DNA strand break cross sections of specific DNA sequences can be efficiently determined using DNA origami nanostructures as platforms exposing the target sequences towards LEEs. In this paper, we systematically study the effect of the oligonucleotide length on the strand break cross section at various irradiation energies. The present work focuses on poly-adenine sequences (d(A 4 ), d(A 8 ), d(A 12 ), d(A 16 ), and d(A 20 )) irradiated with 5.0, 7.0, 8.4, and 10 eV electrons. Independent of the DNA length, the strand break cross section shows a maximum around 7.0 eV electron energy for all investigated oligonucleotides confirming that strand breakage occurs through the initial formation of negative ion resonances. When going from d(A 4 ) to d(A 16 ), the strand break cross section increases with oligonucleotide length, but only at 7.0 and 8.4 eV, i.e., close to the maximum of the negative ion resonance, the increase in the strand break cross section with the length is similar to the increase of an estimated geometrical cross section. For d(A 20 ), a markedly lower DNA strand break cross section is observed for all electron energies, which is tentatively ascribed to a conformational change of the dA 20 sequence. The results indicate that, although there is a general length dependence of strand break cross sections, individual nucleotides do not contribute independently of the absolute strand break cross section of the whole DNA strand. The absolute quantification of sequence specific strand breaks will help develop a more accurate molecular level understanding of radiation induced DNA damage, which can then be used for optimized risk estimates in cancer radiation therapy.

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Section: Oligonucleotide Length Dependence Of Strand Break Cross Sectmentioning

confidence: 99%

Length and Energy Dependence of Low-Energy Electron-Induced Strand Breaks in Poly(A) DNA

Ebel

Bald

2019

IJMS

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Low energy (6–18 eV) electron scattering from condensed thymidine (dT) III: absolute electronic excitation cross sections

Lemelin

Bass

Sanche

2020

Phys. Chem. Chem. Phys.

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Low energy (1–19 eV) electron scattering from condensed thymidine (dT) II: comparison of vibrational excitation cross sections with those of tetrahydrofuran and the recalibrated values of thymine

Abstract: Comparison of absolute vibrational cross sections for low-energy electron scattering from condensed thymidine with those of tetrahydrofuran and thymine.

Cited by 2 publications

References 56 publications

Length and Energy Dependence of Low-Energy Electron-Induced Strand Breaks in Poly(A) DNA

Length and Energy Dependence of Low-Energy Electron-Induced Strand Breaks in Poly(A) DNA

Low energy (6–18 eV) electron scattering from condensed thymidine (dT) III: absolute electronic excitation cross sections

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