Low estradiol responses in oocyte donors undergoing gonadotropin stimulation do not influence clinical outcomes

Abstract

The prevalence of very low E2 responses in donors appears to be high (20.2%). In contrast to autologous IVF cycles, LE2 does not portend poor outcomes in oocyte donors.

Effects of serum estrogen levels before frozen-thawed blastocyst transfer on pregnancy outcomes in hormone replacement cycles

“…This study indicated that in HRT-FET cycles, as long as the endometrial thickness reached 8 mm or more, the estrogen levels on both endometrium transformation day and blastocyst transfer day did not affect pregnancy outcomes. Therefore, we believe that the serum estrogen level before HRT-FET is not significantly associated with pregnancy outcome, which is consistent with other reports 8 – 11 . It has been reported that the high serum E2 level on the endometrium transformation day affects embryo implantation, pregnancy rate, ongoing pregnancy rate and live birth rate for cleavage embryos, but has no adverse effects on frozen blastocyst transfer 18 .…”

“…It is not clear that in HRT-FET cycles, what level the serum estrogen should be maintained at. There are different reports about the effects of E2 level on pregnancy outcome in HRT-FET cycles [8][9][10][11] . High serum estradiol level might lead to gland stroma asynchronization, interfering with endometrial receptivity and affecting pregnancy outcomes 15 .…”

“…Nevertheless, it is still unclear whether the estrogen level is associated with the pregnancy outcomes in HRT cycles. It has been reported that the estrogen level on the endometrium transformation day is not related to pregnancy outcomes in HRT cycles 8 – 11 , serum estrogen level > 100 pg/ml is one of the prerequisites for endometrium transformation 12 , and cancellation of embryo transfer is recommended when the serum estrogen level is less than 75 pg/ml 13 . In this study, we collected clinical data of 708 HRT-FET cycles performed in our hospital from January 2019 to December 2020 and retrospectively analyzed the effects of serum estrogen levels before embryo transfer on pregnancy outcomes, providing a clinical basis for improving HRT-FET protocol.…”

“…Nevertheless, it is still unclear whether the estrogen level is associated with the pregnancy outcomes in HRT cycles. It has been reported that the estrogen level on the endometrium transformation day is not related to pregnancy outcomes in HRT cycles [8][9][10][11] Endometrial preparation and grouping. If B-ultrasound showed that the uterus and ovaries were normal on the third day of menstrual onset or drug withdrawal bleeding, patients started taking 2 mg of progynova twice a day for 4 days, and then 3 mg of progynova twice a day for next 4 days.…”

Minimizing mosaicism: assessing the impact of fertilization method on rate of mosaicism after next-generation sequencing (NGS) preimplantation genetic testing for aneuploidy (PGT-A)

“…Gonadotropin releasing hormone agonist and antagonist in vitro stimulation protocols were included. Ovarian stimulation was monitored with regular ultrasound and serum hormonal assays per practice protocol [19]. Oocyte retrieval was performed by transvaginal ultrasound-guided needle aspiration under intravenous sedation.…”