Low expression of an endopolygalacturonase gene in apple fruit with long-term storage potential

Wakasa, Yuhya; Kudo, Hiroyuki; Ishikawa, Ryuji; Akada, Shinji; Senda, Mineo; Niizeki, Minoru; Harada, Takeo

doi:10.1016/j.postharvbio.2005.10.005

Cited by 131 publications

(96 citation statements)

References 17 publications

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“…This enzyme catalyzes the last step of ethylene production, when ACC (1-aminocyclopropane-1-carboxylate) is converted in ethylene. Probably, the lower ACC oxidase enzyme activity is a result of the lower genes expression related to this enzyme by fruit that received 1-MCP application, once some previously researches suggested that the 1-MCP application reduced the MdACO1 gene expression (WAKASA et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

1-methylcyclopropene effects on volatile profile and quality of 'Royal Gala' apples produced in Southern Brazil and stored in controlled atmosphere

et al. 2015

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Apple volatile aroma depends of complex interaction among organic compounds. The aim of the present study was to evaluate the effect of 1-methylcyclopropene (1-MCP) application on volatile profile and quality on 'Royal Gala' apples produced in Southern Brazil and stored under controlled atmosphere condition (CA; 1.0kPa O2+1.2kPa CO2; 0.5±0.1°C; 94±1% relative humidity). Volatile profile was determined after eight month of CA storage plus 7 days of shelf life via solid-phase microextraction (HS-SPME) and analyzed by gas chromatography coupled to mass spectrometer. In the volatile profile were identified 8 esters, 11 alcohols, 6 aldehydes, 6 acids, 2 ketones, 1 ether and 1 terpene. 1-MCP application reduced significantly the main volatile compounds production by 'Royal Gala' apples produced in Southern Brazil. Its application decreases significantly the esters production, especially 2-methyl-butyl acetate and hexyl acetate, compared to 'Royal Gala' apples storage under CA. 1-methylcyclopropene application decrease ethylene production and respiration rate. Apples treated with 1-MCP exhibit higher hexanal and 2(E)-hexenal production.

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Section: Resultsmentioning

confidence: 99%

1-methylcyclopropene effects on volatile profile and quality of 'Royal Gala' apples produced in Southern Brazil and stored in controlled atmosphere

et al. 2015

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“…A remarkable fact is that fruits that received AVG + 1-MCP and AVG + ABS exhibited the highest respiration at 2 exhibited the highest ACC oxidase enzyme activity; fruit that received AVG, AVG + NAA and AVG + ABS had intermediary activity; and fruit submitted to AVG + 1-MCP presented the lowest enzyme activity ( Figure 2A). Perhaps, the lower ACC oxidase enzyme activity on fruits submitted to AVG + 1-MCP is related to the lower MdACO1 gene expression, considering that 1-MCP application decreased this gene expression in 'Fuji' and 'Golden Delicious' apples (Wakasa et al, 2006;Tatsuki et al, 2007). However, other researchers suggest that the 1-MCP did not decrease the MdACO1 gene expression in 'Orin' (Tatsuki et al, 2007) nor in 'Granny Smith' apples (Asif et al, 2009).…”

Section: Quality Analysis After 8 Months Of Storagementioning

confidence: 94%

Aminoethoxyvinylglycine: isolated and combined with other growth regulators on quality of ‘Brookfield’ apples after storage

Brackmann

Thewes

Anese

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Growth regulators are used in the production of apples worldwide, especially to extend the harvest period and maintain postharvest quality. This study aimed to investigate the effects of applying aminoethoxyvinylglycine (AVG) in isolation as well as in combination with other growth regulators and postharvest techniques on the harvest quality and storage potential of 'Brookfield' apples (Malus domestica), a 'Gala' strain. Fruit receiving AVG only had the highest starch content and the highest titratable acidity at harvest. After 8 months of storage, the AVG + 1-MCP (1-methylcyclopropene) and AVG + ABS (ethylene absorption) conserved higher flesh firmness than to all the other treatments. Naphthaleneacetic acid (NAA) application induced ACC oxidase enzyme activity at harvest, but not after storage. AVG application, with or without the aid of another technique, did not decrease the red skin color of 'Brookfield' apples. Low mealiness and a high healthy fruit percentage was obtained when the fruits were submitted to pre-harvest AVG application combined with NAA, 1-MCP and ABS. Internal carbon dioxide had an inverse correlation with the quantity of healthy fruit and was directly correlated with mealiness.Keywords: Malus domestica, postharvest quality, skin color, ethephon, naphthaleneacetic acid IntroductionThe application of growth regulators is necessary either to advance or to delay the fruit harvest in the field, due to the short period that fruit remains naturally at the correct maturation stage. Besides the short harvest period, another problem is that the harvest is carried out manually and requires a lot of time and manual labor. Thus, growth regulators are frequently used in the orchards.The main growth regulator applied in the field is aminoethoxyvinylglycine (AVG) which is used to delay the harvest in order to avoid pre-harvest fruit drop (McFadyen et al., 2012;Yildiz et al., 2012). AVG blinds the active site of ACC (1-aminocyclopropane-1-carboxylate) synthase enzyme and inhibits ethylene production and maturation events initiated by this plant hormone (Yu and Yang, 1979;Huai et al., 2001). Despite the benefits promoted by AVG application, it decreases the red skin color of 'Gala' apples (Malus domestica) (Steffens et al., 2006). However, this reduction of red skin color has not yet been proven in 'Gala' mutants.Another growth regulator used extensively is ethephon, mainly to advance the fruit harvest. Its application allows for an early harvest of the fruit, and also increases the red color of fruit skin Steffens et al., 2006;Ban et al., 2007). However, ethephon application may decrease organoleptic quality and decrease the storage potential of fruit after harvest, especially in terms of declines in flesh firmness (Steffens et al., 2006;Ban et al., 2007). As well as the application of ethephon, naphthaleneacetic acid (NAA) is also used during apple production. Its application reduces pre-harvest fruit drop in apples (Yuan and Carbaugh, 2007; Unrath et al., 2009), oranges (Zur and Goren, 1977) ...

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“…Of these ACO isoforms, ACO1 is predominantly expressed in ripening apple fruit (Wakasa et al 2006). The ACO1 locus was mapped to LG10 in the apple genome (Costa et al 2005).…”

mentioning

confidence: 99%

Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short Communication

Maric¹

2016

Hort. Sci. (Prague)

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Apple is a climacteric fruit of economic importance and high nutritional value. Better understanding of the apple ripening process is needed to improve fruit quality and extend apple shelf life. This paper presents the results of molecular cloning and characterization of a newly identified allele of the ripening-specific ACO1 gene from Malus floribunda 821. A polymorphism of the ACO1 gene was detected using the polymerase chain reaction and BamH1 and RsaI enzymatic digestion. Whilst cloning and sequencing data confirmed the identity of allele d, it also revealed high sequence conservation (97.3 to 98.9%). In addition, a comparison of sequence data revealed some differences within the coding regions of the ACO1 alleles identified so far.

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Low expression of an endopolygalacturonase gene in apple fruit with long-term storage potential

Cited by 131 publications

References 17 publications

1-methylcyclopropene effects on volatile profile and quality of 'Royal Gala' apples produced in Southern Brazil and stored in controlled atmosphere

1-methylcyclopropene effects on volatile profile and quality of 'Royal Gala' apples produced in Southern Brazil and stored in controlled atmosphere

Aminoethoxyvinylglycine: isolated and combined with other growth regulators on quality of ‘Brookfield’ apples after storage

Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short Communication

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