Low-level laser irradiation effect on endothelial cells under conditions of hyperglycemia

Abstract

Diabetes mellitus is considered to be a very serious lifestyle disease leading to cardiovascular complications and impaired wound healing observed in the diabetic foot syndrome. Chronic hyperglycemia is the source of the endothelial activation. The inflammatory process in diabetes is associated with the secretion of inflammatory cytokines by endothelial cells, e.g., tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6). The method of phototherapy using laser beam of low power (LLLT—low-level laser therapy) effectively supports the conventional treatment of diabetic vascular complications such as diabetic foot syndrome. The aim of our study was to evaluate the effect of low-power laser irradiation at two wavelengths (635 and 830 nm) on the secretion of inflammatory factors (TNF-α and IL-6) by the endothelial cell culture—HUVEC line (human umbilical vein endothelial cell)—under conditions of hyperglycemia. It is considered that adverse effects of hyperglycemia on vascular endothelial cells may be corrected by the action of LLLT, especially with the wavelength of 830 nm. It leads to the reduction of TNF-α concentration in the supernatant and enhancement of cell proliferation. Endothelial cells play an important role in the pathogenesis of diabetes; however, a small number of studies evaluate an impact of LLLT on these cells under conditions of hyperglycemia. Further work on this subject is warranted.

Cell Adhesion Molecules Are Mediated by Photobiomodulation at 660 nm in Diabetic Wounded Fibroblast Cells

“…A diabetic wounded model was achieved by continuously growing cells under hyperglycemic conditions; cells were cultured in complete media (basal glucose concentration of 5.6 mMol/L) containing an additional 17 mMol/L d -glucose [ 43 , 44 ]. Mimicking a diabetic condition using high glucose concentrations of 20–40 mM/L is not uncommon in inducing cellular changes in different cell types in vitro [ 45 , 46 , 47 , 48 ]. For experiments, 6 × 10 5 cells in 3 mL complete media were seeded into 3.4 cm diameter tissue culture plates and cells allowed to adhere during overnight incubation.…”

Estimulação Óssea Através De Ultrasson

“…MOFFATT; SHUPP, 2012). O método de fototerapia usando raio laser de baixa intensidade suporta efetivamente o tratamento convencional e traz uma melhoria significativa na qualidade de vida em pacientes portadores da diabetes (GÓRALCZYK et al, 2016).…”

Lactobacillus and Bifidobacteria Probiotic Strains Improve Glycemic and Inflammation Profiles in Obesity Model in Mice

“…There was also absent the FR of the PEMs of these mice. According to the literature data, chronic hyperglycemia can trigger lipid peroxidation, decrease the stability of the antioxidant system, and damage the vascular endothelium [32,33], resulting in increased levels of proinflammatory cytokine, like TNF-α and other markers of systemic inflammation in the blood, as well as the functional activity of macrophages [34][35][36][37][38]. Since the level of TNF-α excretion is characterized by a clear positive correlation with the degree of obesity and insulin resistance, the correction of products of this proinflammatory cytokine is considered by many researchers as a promising therapeutic approach to the treatment of obesity and insulin resistance [34].…”