Low-level laser therapy (LLLT) acts as cAMP-elevating agent in acute respiratory distress syndrome

Lima, Flávia Mafra de; Moreira, Leonardo Marmo; Villaverde, Antonio; Albertini, Regiane; Castro‐Faria‐Neto, Hugo C.; Aimbire, Flávio

doi:10.1007/s10103-010-0874-x

Cited by 43 publications

(38 citation statements)

References 33 publications

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“…Several workers have shown an increase in adenosine-3’,5’-cyclic-monophosphate (cAMP) after PBM [54], [55]. Although it is tempting to suppose that this increase in cAMP is a direct consequence of the rise in ATP caused by light, firm evidence for this connection is lacking.…”

Section: Molecular Mechanisms Of Pbmmentioning

confidence: 99%

Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy

Freitas

Hamblin

2016

IEEE J. Select. Topics Quantum Electron.

1,069

796

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Photobiomodulation (PBM) also known as low-level laser (or light) therapy (LLLT), has been known for almost 50 years but still has not gained widespread acceptance, largely due to uncertainty about the molecular, cellular, and tissular mechanisms of action. However, in recent years, much knowledge has been gained in this area, which will be summarized in this review. One of the most important chromophores is cytochrome c oxidase (unit IV in the mitochondrial respiratory chain), which contains both heme and copper centers and absorbs light into the near-infra-red region. The leading hypothesis is that the photons dissociate inhibitory nitric oxide from the enzyme, leading to an increase in electron transport, mitochondrial membrane potential and ATP production. Another hypothesis concerns light-sensitive ion channels that can be activated allowing calcium to enter the cell. After the initial photon absorption events, numerous signaling pathways are activated via reactive oxygen species, cyclic AMP, NO and Ca2+, leading to activation of transcription factors. These transcription factors can lead to increased expression of genes related to protein synthesis, cell migration and proliferation, anti-inflammatory signaling, anti-apoptotic proteins, antioxidant enzymes. Stem cells and progenitor cells appear to be particularly susceptible to LLLT.

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Section: Molecular Mechanisms Of Pbmmentioning

confidence: 99%

Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy

Freitas

Hamblin

2016

IEEE J. Select. Topics Quantum Electron.

1,069

796

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“…BDNF promotes the survival of neurons and dendrite growth both in vitro and in vivo (Katz and Shatz, 1996;Connor and Dragunow, 1998;Murer et al, 2001). A growing body of evidence indicates that BDNF levels are decreased in brains of AD patients (Hu and Russek, 2008;Zuccato and Cattaneo, 2009) and APP transgenic mice .…”

Section: Lllt Increases Bdnf Protein Levels In Hippocampal Neuronsmentioning

confidence: 99%

“…In addition to their classical role in supporting survival of neuronal populations, brain-derived neurotrophic factor (BDNF), in particular, is a strong candidate to modulate dendritic structure and potentiate synaptic transmission in the CNS (Katz and Shatz, 1996;Connor and Dragunow, 1998;Murer et al, 2001). In patients with AD, neurites atrophy and synaptic loss are considered the major causes of cognitive impairment (Einstein et al, 1994;Masliah et al, 2001;Selkoe, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Low-Level Laser Therapy Rescues Dendrite Atrophy via Upregulating BDNF Expression: Implications for Alzheimer's Disease

2013

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Downregulation of brain-derived neurotrophic factor (BDNF) in the hippocampus occurs early in the progression of Alzheimer's disease (AD). Since BDNF plays a critical role in neuronal survival and dendrite growth, BDNF upregulation may contribute to rescue dendrite atrophy and cell loss in AD. Low-level laser therapy (LLLT) has been demonstrated to regulate neuronal function both in vitro and in vivo.In the present study, we found that LLLT rescued neurons loss and dendritic atrophy via upregulation of BDNF in both A␤-treated hippocampal neurons and cultured APP/PS1 mouse hippocampal neurons. Photoactivation of transcription factor CRE-binding protein (CREB) increased both BDNF mRNA and protein expression, since knockdown CREB blocked the effects of LLLT. Furthermore, CREBregulated transcription was in an ERK-dependent manner. Inhibition of ERK attenuated the DNA-binding efficiency of CREB to BDNF promoter. In addition, dendrite growth was improved after LLLT, characterized by upregulation of Rac1 activity and PSD-95 expression, and the increase in length, branching, and spine density of dendrites in hippocampal neurons. Together, these studies suggest that upregulation of BDNF with LLLT by activation of ERK/CREB pathway can ameliorate A␤-induced neurons loss and dendritic atrophy, thus identifying a novel pathway by which LLLT protects against A␤-induced neurotoxicity. Our research may provide a feasible therapeutic approach to control the progression of AD.

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“…The positive effects of LLLT in the modulation of the inflammatory process were also demonstrated by Oliveira et al [35] in the experimental model of sepsis induced by lipopolysaccharide. de Lima et al [36] also demonstrated a positive effect of LLLT on the inflammatory process in the same model of sepsis.…”

Section: Discussionmentioning

confidence: 89%

Laser therapy modulates systemic inflammatory processes and muscle atrophy in an experimental model of sepsis in rats

Assis

Rennó

Bublitz

et al. 2015

Photonics &Amp; Lasers in Medicine

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Objective: The aim of this study was to determine the effectiveness of low-level laser therapy (LLLT) on the modulation of the systemic inflammatory processes and skeletal muscle morphology in an experimental sepsis model (cecal ligation and puncture, CLP). Study design: Seventy-two male Wistar rats were randomly divided into three groups: control group (CG); sepsis group (SG) where rats were submitted to CLP but without LLLT treatment, and the sepsis laser-treated group (SLG). Laser irradiation (GaAlAs laser, continuous wave, 808 nm, 30 mW, 48 s, 30 J/cm 2 , 0.028 cm 2 , 1.07 mW/cm 2 ) was performed immediately after surgery and every 24 h at 4 points (on the middle of tibialis anterior and diaphragm, bilaterally), through the punctual contact technique. All sepsis animals were sacrificed at 6, 24, 48 and 72 h postsurgery. The immunohistochemistry analysis was used to verify the expression of proteins related to the regulation of muscle wasting (MuRF-1 and atrogin). In order to investigate the action of LLLT on inflammatory mediators in the rat sepsis model, two inflammatory cytokines IL-6 and IL-10, were evaluated. Results:The results showed that the laser-treated animals presented a lower IL-6 activity and decreased atrogin and MuRF-1 immunoexpression. However, no difference was observed in muscle cross-sectional area between the experimental groups. Conclusion: These results suggest that LLLT was able to decrease the systemic inflammation and muscle atrophy markers, preventing muscle protein degradation. Zusammenfassung Zielsetzung: Ziel dieser Studie war es, die Wirksamkeit der Low-Level-Laser-Therapie (LLLT) auf die Modulation der systemischen Entzündungsprozesse und Skelettmuskel-Morphologie in einem experimentellen Sepsismodell (Blinddarm-Ligation und Punktions-induzierte Sepsis, CLP) zu untersuchen. Studiendesign: 72 männliche Wistar-Ratten wurden nach dem Zufallsprinzip in drei Gruppen eingeteilt: Kontrollgruppe (CG); Sepsis-Gruppe (SG) mit Ratten, die einer CLP aber keiner LLLT-Behandlung unterzogen wurden; und Sepsis + Laser-behandelte Gruppe (SLG). Die Laserbestrahlung (kontinuierlich abstrahlender GaAlAs-Laser mit 808 nm, 30 mW, 48 s, 30 J/cm 2 , 0,028 cm 2 , 1.07 mW/cm 2 ) erfolgte mittels punktueller Kontakttechnik Brought to you by | Kungliga Tekniska Högskolan Authenticated Download Date | 8/8/15 7:21 AM 242 L. Assis et al.: Laser therapy modulates systemic inflammatory processesunmittelbar nach der Operation und alle 24 h an 4 ausgewählten Punkten (in der Mitte des M. tibialis anterior und Zwerchfell, bilateral). Alle Sepsis-Tiere (Gruppen SG und SLG) wurden 6, 24, 48 und 72 h nach der Operation euthanasiert. Zur Verifizierung der Expression der mit der Regulierung von Muskelschwund in Verbindung stehenden Proteine (MuRF-1 und Atrogin) wurde die immunhistochemische Analyse verwendet. Um die Wirkung der LLLT auf die Entzündungsmediatoren im Ratten-Sepsismodell zu untersuchen, wurden zwei inflammatorische Zytokine (IL-6 und IL-10) evaluiert. Ergebnisse: Im Ergebnis wurde bei den Laser-behandelten...

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Low-level laser therapy (LLLT) acts as cAMP-elevating agent in acute respiratory distress syndrome

Cited by 43 publications

References 33 publications

Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy

Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy

Low-Level Laser Therapy Rescues Dendrite Atrophy via Upregulating BDNF Expression: Implications for Alzheimer's Disease

Laser therapy modulates systemic inflammatory processes and muscle atrophy in an experimental model of sepsis in rats

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