Low-Level Laser Therapy with 810 nm Wavelength Improves Skin Wound Healing in Rats with Streptozotocin-Induced Diabetes

Abstract

Objective: The aim of present study was to evaluate whether low-level laser therapy (LLLT) can reverse the impaired wound healing process in diabetic rats. Background data: Impaired wound healing in diabetic patients represents a major health problem. Recent studies have indicated that LLLT may improve wound healing in diabetic rats, but the optimal treatment parameters are still unknown. Materials and methods: Male SpragueDawley rats (n = 21) were randomly divided into three groups: a healthy control group, a diabetic sham-treated group, and a diabetic LLLT-treated group. Diabetes mellitus was then induced by streptozotocin administration to the two diabetic groups. One 4 cm long full thickness skin incision and one full thickness circular excision (diameter = 4 mm) were performed on the back of each rat. An infrared 810 nm laser with an output of 30 mW, a power density of 30 mW/cm 2 , and a spot size of 1 cm 2 was used to irradiate each wound for 30 sec (daily dose of 0.9 J/cm 2 /wound/day). Results: In diabetic rats, the histology of LLLT-treated excisions revealed a similar healing response to that in nondiabetic controls, with significantly more mature granulation tissue than in the sham-treated diabetic control group. LLLT reduced the loss of tensile strength, and increased the incision wound stiffness significantly compared with sham-irradiated rats, but this did not achieve the same level as in the nondiabetic controls. Conclusions: Our study demonstrates that infrared LLLT can improve wound healing in diabetic rats. Nevertheless, further research needs to be performed to evaluate the exact underlying mechanism and to further optimize LLLT parameters for clinical use.

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

“…The PBM of 810 nm irradiation improve neurological performance in traumatic brain injury [16], accelerate both normal and chronic dermal wound healing [17–20] and prevent the appearance of surgical scar [21]. Furthermore, 810 nm laser irradiation exhibited an anti-inflammatory effect on murine bone marrow derived dendritic cells that could be governed by cyclic adenosine monophosphate (cAMP) and reduced nuclear factor (NF)-kB signaling [22].…”

Low‐level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

“…Nevertheless, there is a lack of studies that focus on the maturation and differentiation of the wounded epithelium after LLLT and throughout the healing process, although a study has demonstrated that the use of green LEDs increased the production of HB-EGF and VEGF [15], which do promote the migration of keratinocytes [16]. Most of the published literature is concerned with the clinical and morphological response of wound healing [17, 18] or the response of mesenchymal cells in either soft [19, 20] or hard tissues [21, 22] in tissue repair.…”

Effects of the led therapy on the global DNA methylation and the expression of Dnmt1 and Dnmt3a genes in a rat model of skin wound healing

“…In vitro previous studies associated the low-level laser therapy to the modulation of cellular mechanisms such as DNA synthesis and gene expression [4,5], production of fibrotic cells [6], stimulation of cell proliferation [7][8][9], and cell differentiation [10][11][12][13], thus providing evidences to support a hypothesis of the implication of epigenetic mechanisms in the molecular responses that are evoked in the cells by the exposure to light sources [14].…”