Low Molecular Weight Polyethyleneimine Conjugated to Magnetic Nanoparticles as a Vector for Gene Delivery

Delyagina, Evgenya; Li, Wenzhong; Schade, Anna; Kuhlo, Anna‐L.; Ma, Nan; Steinhoff, Gustav

doi:10.1063/1.3530058

Cited by 4 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with high molecular weight PEI, low molecular weight PEI possesses weak ability for compressing plasmid DNA because of its low cation density while its cytotoxicity is acceptable. 555 Therefore, crosslinking low molecular weight PEI via inert polymers or crosslinking reagents has become one of the most promising strategies for enhancing the cationic charge of this low molecular weight PEI, and further for promoting its ability to condense DNA. Forrest et al synthesized a degradable PEI derivative by combining PEI 800 Da with a diacrylate crosslinker.…”

Section: Non-viral Gene Carriers In Gene Delivery For the Endothelial...mentioning

confidence: 99%

Surface modification and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications

et al. 2015

Self Cite

View full text Add to dashboard Cite

Surface modification and endothelialization of vascular biomaterials are common approaches that are used to both resist the nonspecific adhesion of proteins and improve the hemocompatibility and long-term patency of artificial vascular grafts. Surface modification of vascular grafts using hydrophilic poly(ethylene glycol), zwitterionic polymers, heparin or other bioactive molecules can efficiently enhance hemocompatibility, and consequently prevent thrombosis on artificial vascular grafts. However, these modified surfaces may be excessively hydrophilic, which limits initial vascular endothelial cell adhesion and formation of a confluent endothelial lining. Therefore, the improvement of endothelialization on these grafts by chemical modification with specific peptides and genes is now arousing more and more interest. Several active peptides, such as RGD, CAG, REDV and YIGSR, can be specifically recognized by endothelial cells. Consequently, graft surfaces that are modified by these peptides can exhibit targeting selectivity for the adhesion of endothelial cells, and genes can be delivered by targeting carriers to specific tissues to enhance the promotion and regeneration of blood vessels. These methods could effectively accelerate selective endothelial cell recruitment and functional endothelialization. In this review, recent developments in the surface modification and endothelialization of biomaterials in vascular tissue engineering are summarized. Both gene engineering and targeting ligand immobilization are promising methods to improve the clinical outcome of artificial vascular grafts.

show abstract

Section: Non-viral Gene Carriers In Gene Delivery For the Endothelial...mentioning

confidence: 99%

Surface modification and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

Investigation of the Sequential Actions of Doxorubicin and p53 on Tumor Cell Growth Via Branched Polyethylenimine-β-cyclodextrin Conjugates

et al. 2016

View full text Add to dashboard Cite

The combination of gene therapy and chemotherapy has showed increased therapeutic efficacy in the treatment of cancers, but it is not well investigated about the actual coordination pattern between therapeutic gene and chemical drug. In this work, p53/BPEI-β-CD/AD-dox complex was fabricated and employed to investigate the interaction manner between p53 and doxorubicin (Dox). Briefly, branched polyethylenimine (BPEI) was conjugated with β-cyclodextrin hydrate (β-CD) to form BPEI-β-CD backbone, and p53 plasmid was condensed by positively charged BPEI via electrostatic interaction, while Dox was first conjugated with adamantine (AD) and then assembled with BPEI-β-CD backbone via the host-guest interaction. It was found that the BPEI-β-CD backbone possessed high endocytosis efficiency but low cytotoxicity. Moreover, p53/BPEI-β-CD/AD-dox complex released Dox and enabled the expression of p53 gene in a sequential manner, and the released Dox and expressed p53 gene showed successive inhibition of the growth of HeLa cells in vitro. With the ability to co-deliver chemical drug and therapeutic gene and exert their inhibitory actions on tumor cell growth in a sequential manner, this DNA/BPEI-β-CD/AD-drug complex via electrostatic interaction and host-guest assembly not only achieved long-term efficacy in inhibiting tumor cell growth but also can be employed as a platform to investigate the coordination pattern between chemical drugs and therapeutic genes for other purposes.

show abstract

Magnetic Targeting Strategies in Gene Delivery

Delyagina

et al. 2011

Nanomedicine

View full text Add to dashboard Cite

Gene delivery is a process of the insertion of transgenes into cells with the purpose to obtain the expression of encoded protein. The therapeutic application of this process is termed gene therapy, which is becoming a promising instrument to treat genetic and acquired diseases. Although numerous methods of gene transfer have already been developed, including biological, physical and chemical approaches, the optimal strategy has to be discovered. Importantly, it should be effective, selective and safe to be translated to the clinic. Magnetic targeting has been demonstrated as an effective strategy to decrease side effects of gene transfer, while increasing the selectivity and efficiency of the applied vector. This article will focus on the latest progress in the development of different magnetic vectors, based on both viral and nonviral gene delivery agents. It will also include a description of magnetic targeting applications in stem cells and in vivo, which has gained interest in recent years due to the rapid development of technology.

show abstract

Low Molecular Weight Polyethyleneimine Conjugated to Magnetic Nanoparticles as a Vector for Gene Delivery

Cited by 4 publications

References 0 publications

Surface modification and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications

Surface modification and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications

Investigation of the Sequential Actions of Doxorubicin and p53 on Tumor Cell Growth Via Branched Polyethylenimine-β-cyclodextrin Conjugates

Magnetic Targeting Strategies in Gene Delivery

Contact Info

Product

Resources

About