Low oxygen concentrations improve yak oocyte maturation and enhance the developmental competence of preimplantation embryos

He, Honghong; Zhang, Huizhu; Li, Qin; Fan, Jinjiang; Zhang, Tongxiang; Niayale, Robert; Zhao, Ling; Hu, Xuequan; Han, Xiaobin; Yang, Shanshan; Cui, Yan; Yu, Sijiu

doi:10.1016/j.theriogenology.2020.06.022

Cited by 16 publications

(11 citation statements)

References 68 publications

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“…Then, the COCs were randomly divided into four groups, and approximately 40–50 COCs were cultured in 400 µl of IVM medium in four‐well dishes (Nunc, Roskilde, Denmark) and cultured with mineral oil overlay. The COCs matured in incubators with different oxygen concentrations (20%, 10%, 5% or 1% O 2 ) and a balance of nitrogen, at 38°C in ambient humidified air with 5% CO 2 for 24 h (He et al., 2020). After 24 h of maturation, the morphology of COCs and expansion of cumulus cells were observed under light microscope (Nikon).…”

Section: Methodsmentioning

confidence: 99%

Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF‐1 and VEGF

Zhang

et al. 2022

Reprod Domestic Animals

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The gas-phase environment of in vitro culture system plays an important role in the development of oocytes, and oxygen concentration is one of the important factors. In the present study, we aimed to explore the effect of different oxygen concentrations (20%, 10%, 5% or 1% O 2 ) in yak oocyte maturation and to detect the expression of hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF) and cell apoptosis in yak COCs. First, the maturation rate of oocytes, cleavage rate and blastocysts rate following parthenogenetic activation in the group with 5% oxygen concentration were significantly higher (p < .05) than the other groups. Then, TUNEL analysis showed that the 5% oxygen concentration group significantly inhibited apoptosis of cumulus-oocyte complexes (COCs) compared to the other group, and the transcription and protein levels of pro-apoptotic factor Bax, HIF-1α and VEGF in yak COCs significantly reduced, while anti-apoptotic factor Bcl-2 significantly increased.Furthermore, immunohistochemical staining results indicated that HIF-1α protein was mainly located in theca follicle interna, mural follicular stratum granulosum, corona radiata and ovarian stroma in the follicular ovarian tissue, while VEGF protein was mainly located in the granulosa and theca cell layers. In summary, our findings demonstrate that 5% oxygen concentration may promote maturation and inhibit apoptosis of oocytes through HIF-1α-mediated VEGF expression.

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Section: Methodsmentioning

confidence: 99%

Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF‐1 and VEGF

Zhang

et al. 2022

Reprod Domestic Animals

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“…Ovaries were collected from a commercial slaughterhouse (Xining, Qinghai, China) and stored at 30°C in sterile saline (0.9% NaCl) with two antibiotics (100 IU/mL penicillin and 0.05 mg/mL streptomycin), and sent to the laboratory within 4 h ( 4 , 27 ). Upon arrival at the laboratory, ovarian samples were cleaned three times with sterile saline containing two antibiotics at 35°C to minimize external contamination.…”

Section: Methodsmentioning

confidence: 99%

Leukemia inhibitory factor enhances the development and subsequent blastocysts quality of yak oocytes in vitro

Zhao

Ding

et al. 2022

Front. Vet. Sci.

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Leukemia inhibitory factor (LIF) is a multipotent cytokine of the IL-6 family which plays a critical role in the maturation and development of oocytes. This study evaluated the influence of LIF on the maturation and development ability of yak oocytes, and the quality of subsequent blastocysts under in vitro culture settings. Different concentrations of LIF (0, 25, 50, and 100 ng/mL) were added during the in vitro culture of oocytes to detect the maturation rate of oocytes, levels of mitochondria, reactive oxygen species (ROS), actin, and apoptosis in oocytes, mRNA transcription levels of apoptosis and antioxidant-related genes in oocytes, and total cell number and apoptosis levels in subsequent blastocysts. The findings revealed that 50 ng/mL LIF could significantly increase the maturation rate (p < 0.01), levels of mitochondria (p < 0.01) and actin (p < 0.01), and mRNA transcription levels of anti-apoptotic and antioxidant-related genes in yak oocytes. Also, 50 ng/mL LIF could significantly lower the generation of ROS (p < 0.01) and apoptosis levels of oocytes (p < 0.01). In addition, blastocysts formed from 50 ng/mL LIF-treated oocytes showed significantly larger total cell numbers (p < 0.01) and lower apoptosis rates (p < 0.01) than the control group. In conclusion, the addition of LIF during the in vitro maturation of yak oocytes improved the quality and the competence of maturation and development in oocytes, as well as the quality of subsequent blastocysts. The result of this study provided some insights into the role and function of LIF in vitro yak oocytes maturation, as well as provided fundamental knowledge for assisted reproductive technologies in the yak.

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“…All experimental animals were handled according to the Animal Ethics Procedures and Guidelines of the People's Republic of China, and the study was approved by the Institutional Animal Care and Use Committee (IACUC) of the College of Veterinary Medicine of Gansu Agricultural University. The maturation culture of oocytes was carried out in accordance with the previous methods used in our laboratory (He et al, 2020). Briefly, ovaries were collected from slaughterhouses (Xining, Qinghai, China) and transported to the lab in 35°C saline (0.9% NaCl).…”

Section: Methodsmentioning

confidence: 99%

Ascorbic acid protects the toxic effects of aflatoxin B₁ on yak oocyte maturation

Zhao

et al. 2022

Animal Science Journal

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High-quality oocytes are a prerequisite for successful fertilization. Mammals feeding on aflatoxin-contaminated feed can cause reproductive toxicity, including follicular atresia, poor oocyte development and maturation, and aberrant epigenetic modifications of oocytes. In addition, the important role of ascorbic acid (AA) in reproductive biology has been confirmed, and AA is widely used as an antioxidant in cell culture.

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Low oxygen concentrations improve yak oocyte maturation and enhance the developmental competence of preimplantation embryos

Cited by 16 publications

References 68 publications

Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF‐1 and VEGF

Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF‐1 and VEGF

Leukemia inhibitory factor enhances the development and subsequent blastocysts quality of yak oocytes in vitro

Ascorbic acid protects the toxic effects of aflatoxin B₁ on yak oocyte maturation

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Low oxygen concentrations improve yak oocyte maturation and enhance the developmental competence of preimplantation embryos

Cited by 16 publications

References 68 publications

Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF‐1 and VEGF

Low oxygen concentration improves yak oocyte maturation and inhibits apoptosis through HIF‐1 and VEGF

Leukemia inhibitory factor enhances the development and subsequent blastocysts quality of yak oocytes in vitro

Ascorbic acid protects the toxic effects of aflatoxin B1 on yak oocyte maturation

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Product

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Ascorbic acid protects the toxic effects of aflatoxin B₁ on yak oocyte maturation