Low pH-responsive proteins revealed by a 2-DE based MS approach and related physiological responses in Citrus leaves

Zhang, Jiang; Li, Qiang; Qi, Yi-Ping; Huang, Wei-Lin; Yang, Lin-Tong; Lai, Ning-Wei; Ye, Xin; Chen, Li‐Song

doi:10.1186/s12870-018-1413-3

Cited by 18 publications

(20 citation statements)

References 67 publications

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“…At least two of matched peptides were necessary for each protein. Protein identifications were accepted if MASCOT score was ≥ 70, and the sequence coverage was ≥ 20% or the number of matched peptides (NMP) was ≥ five [84,85]. DAPs were classified according to KEGG (http://www.kegg.jp/), GO (http://www.geneontology.org/) and Uniprot (http://www.uniprot.org/) databases [86,87].…”

Section: Maldi-tof/tof-ms-based Protein Identification and Bioinformamentioning

confidence: 99%

Excess Copper-Induced Alterations of Protein Profiles and Related Physiological Parameters in Citrus Leaves

Huang

et al. 2020

Plants

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This present study examined excess copper (Cu) effects on seedling growth, leaf Cu concentration, gas exchange, and protein profiles identified by a two-dimensional electrophoresis (2-DE) based mass spectrometry (MS) approach after Citrus sinensis and Citrus grandis seedlings were treated for six months with 0.5 (control), 200, 300, or 400 μM CuCl2. Forty-one and 37 differentially abundant protein (DAP) spots were identified in Cu-treated C. grandis and C. sinensis leaves, respectively, including some novel DAPs that were not reported in leaves and/or roots. Most of these DAPs were identified only in C. grandis or C. sinensis leaves. More DAPs increased in abundances than DAPs decreased in abundances were observed in Cu-treated C. grandis leaves, but the opposite was true in Cu-treated C. sinensis leaves. Over 50% of DAPs were associated with photosynthesis, carbohydrate, and energy metabolism. Cu-toxicity-induced reduction in leaf CO2 assimilation might be caused by decreased abundances of proteins related to photosynthetic electron transport chain (PETC) and CO2 assimilation. Cu-effects on PETC were more pronounced in C. sinensis leaves than in C. grandis leaves. DAPs related to antioxidation and detoxification, protein folding and assembly (viz., chaperones and folding catalysts), and signal transduction might be involved in Citrus Cu-toxicity and Cu-tolerance.

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Section: Maldi-tof/tof-ms-based Protein Identification and Bioinformamentioning

confidence: 99%

Excess Copper-Induced Alterations of Protein Profiles and Related Physiological Parameters in Citrus Leaves

Huang

et al. 2020

Plants

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“…Considerable research has been performed to clarify the mechanisms of Al-toxicity and -tolerance in plants [5–11]. Nevertheless, limited data are available on the responses of plants to low pH [12–14]. Although some plants grow well in acidic soils with high level of active Al, the adaptive mechanisms of plants to low pH and Al-toxicity are not exactly the same [3, 15].…”

Section: Introductionmentioning

confidence: 99%

Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves

Long

Huang

et al. 2019

BMC Plant Biol

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BackgroundLimited data are available on the responses of reactive oxygen species (ROS) and methylglyoxal (MG) metabolisms to low pH in roots and leaves. In China, quite a few of Citrus are cultivated in acidic soils (pH < 5.0). ‘Xuegan’ (Citrus sinensis) and ‘Sour pummelo’ (Citrus grandis) (C. sinensis were more tolerant to low pH than C. grandis) seedlings were irrigated daily with nutrient solution at a pH of 2.5, 3 or 5 for nine months. Thereafter, we examined low pH effects on growth, and superoxide anion production rate (SAP), malondialdehyde (MDA), MG, antioxidants, and enzymes related to ROS and MG detoxification in roots and leaves in order to (a) test the hypothesis that low pH affected ROS and MG metabolisms more in roots than those of leaves, and (b) understand the roles of ROS and MG metabolisms in Citrus low pH-tolerance and -toxicity.ResultsCompared with control, most of the physiological parameters related to ROS and MG metabolisms were greatly altered at pH 2.5, but almost unaffected at pH 3. In addition to decreased root growth, many fibrous roots became rotten and died at pH 2.5. pH 2.5-induced changes in SAP, the levels of MDA, MG and antioxidants, and the activities of most enzymes related to ROS and MG metabolisms were greater in roots than those of leaves. Impairment of root ascorbate metabolism was the most serious, especially in C. grandis roots. pH 2.5-induced increases in MDA and MG levels in roots and leaves, decreases in the ratios of ascorbate/(ascorbate+dehydroascorbate) in roots and leaves and of reduced glutathione/(reduced+oxidized glutathione) in roots were greater in C. grandis than those in C. sinensis.ConclusionsLow pH affected MG and ROS metabolisms more in roots than those in leaves. The most seriously impaired ascorbate metabolism in roots was suggested to play a role in low pH-induced root death and growth inhibition. Low pH-treated C. sinensis roots and leaves had higher capacity to maintain a balance between ROS and MG production and their removal via detoxification systems than low pH-treated C. grandis ones, thus contribute to the higher acid-tolerance of C. sinensis.

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“…Moreover, soil acidity is becoming an increasing urgent problem in some Citrus orchards [22]. Although the physiological and molecular responses of Citrus to Al-toxicity or low pH have been investigated in some details [9, 23–25], very scarcity is known on Citrus responses to pH and Al interactions. Preliminary study showed that increasing nutrient solution pH prevented Al-toxic effects on Citrus growth and photosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Increasing Nutrient Solution pH Alleviated Aluminum-Induced Inhibition of Growth and Impairment of Photosynthetic Electron Transport Chain in Citrus sinensis Seedlings

Yang

Cai

et al. 2019

BioMed Research International

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Although the physiological and molecular responses of Citrus to Al-toxicity or low pH have been examined in some details, little information is available on Citrus responses to pH and aluminum (Al) interactions. Citrus sinensis seedlings were irrigated for 18 weeks with nutrient solution at a concentration of 0 or 1 mM AlCl3•6H2O and a pH of 2.5, 3.0, 3.5, or 4.0. Thereafter, biomass, root, stem, and leaf concentrations of Al and nutrients, leaf gas exchange, chlorophyll a fluorescence (OJIP) transients, and related parameters were investigated to understand the physiological mechanisms underlying the elevated pH-induced alleviation of Citrus toxicity. Increasing the nutrient solution pH from 2.5 to 4.0 alleviated the Al-toxic effects on biomass, photosynthesis, OJIP transients and related parameters, and element concentrations, uptake, and distributions. In addition, low pH effects on the above physiological parameters were intensified by Al-toxicity. Evidently, a synergism existed between low pH and Al-toxicity. Increasing pH decreased Al uptake per root dry weight and its concentration in roots, stems, and leaves and increased nitrogen, phosphorus, calcium, magnesium, sulfur, and boron uptake per plant and their concentrations in roots, stems, and leaves. This might be responsible for the elevated pH-induced alleviation of growth inhibition and the impairment of the whole photosynthetic electron transport chain, thus preventing the decrease of CO2 assimilation.

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Low pH-responsive proteins revealed by a 2-DE based MS approach and related physiological responses in Citrus leaves

Cited by 18 publications

References 67 publications

Excess Copper-Induced Alterations of Protein Profiles and Related Physiological Parameters in Citrus Leaves

Excess Copper-Induced Alterations of Protein Profiles and Related Physiological Parameters in Citrus Leaves

Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves

Increasing Nutrient Solution pH Alleviated Aluminum-Induced Inhibition of Growth and Impairment of Photosynthetic Electron Transport Chain in Citrus sinensis Seedlings

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