Low resolution meets high: towards a resolution continuum from cells to atoms

The Requirement for Mechanical Coupling Between Head and S2 Domains in Smooth Muscle Myosin ATPase Regulation and its Implications for Dimeric Motor Function

“…The deviations of the two-heptad and three-heptad models are within acceptable limits for docking to the 20 Å resolution cryo electron microscopy data, 23 which is the resolution at which the inhibited-state structure was produced. The conformational change deposits strain energy into both S1 and S2 domains.…”

A 3-D Reconstruction of Smooth Muscle α-Actinin by CryoEm Reveals Two Different Conformations at the Actin-binding Region

“…When the initial rotation of the rod atomic model was varied up to 758 from its ideal location and then fit allowing free movement, the rms deviation from the best position averaged 3.8 Å (range 2.7 Å -5.3 Å ), values well below the resolution of the Smooth Muscle a-Actinin Structure reconstruction but within expectations based on other cryoEM reconstructions. 27 The accuracy in position of the R1 -R4 domain model within the long density of the reconstruction was quantified by starting the refinement at different locations along the rod density and proceeding while fixing this "axial" position, i.e. rotation about the long axis and Z-shift were minimized.…”

Assembly of a Tailed Bacterial Virus and Its Genome Release Studied in Three Dimensions

“…This will be possible because ϕ29 mutant infections yield well-characterized assembly intermediates and because in vitro assembly of ϕ29 rivals in vivo assembly in efficiency (Anderson and Reilly, 1993). More generally, the union of 3D reconstruction with physiological studies of mutants and the extension of structural analysis to higher resolutions (Baker and Johnson, 1996) should yield a better understanding of viral morphogenesis and infection.…”