Low risk of resistance evolution of Spodoptera frugiperda to chlorfenapyr in Brazil

Kanno, Rubens Hideo; Bolzan, Aline Adriana; Kaiser, Ingrid Schimidt; Lira, E. da C.; Amaral, Fernando Semmelroth de Assunção e; Guidolin, Aline Sartori; Nascimento, Antônio Rogério Bezerra do; Omoto, Celso

doi:10.1007/s10340-019-01165-x

Cited by 15 publications

(13 citation statements)

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“…Also, a low level of resistance was recorded for the carbamate thiodicarb for Sf_Des supporting the recommendation of this compound for soybean seed treatment to control early damage by FAW 57 . Chlorfenapyr also has shown a lack of resistance towards the Sf_Des strain, confirming the results recently published for FAW from Brazil 58 . Chlorfenapyr is a pro‐insecticide, which has to be activated by P450 enzymes 59 .…”

Section: Discussionsupporting

confidence: 80%

“…Chlorfenapyr is a pro‐insecticide, which has to be activated by P450 enzymes 59 . Therefore, the hypothesis that the overall high activity of P450 enzymes might contribute to chlorfenapyr toxicity even in resistant insects, has been considered previously 58, 60 . Indoxacarb also is a pro‐insecticide, yet it is activated by esterases through cleavage of the N ‐carbomethoxy group, resulting in an active metabolite that potently blocks the VGSC 61 …”

Section: Discussionmentioning

confidence: 99%

“… 57 Chlorfenapyr also has shown a lack of resistance towards the Sf_Des strain, confirming the results recently published for FAW from Brazil. 58 Chlorfenapyr is a pro‐insecticide, which has to be activated by P450 enzymes. 59 Therefore, the hypothesis that the overall high activity of P450 enzymes might contribute to chlorfenapyr toxicity even in resistant insects, has been considered previously.…”

Section: Discussionmentioning

confidence: 99%

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Toxicological and molecular profiling of insecticide resistance in a Brazilian strain of fall armyworm resistant to Bt Cry1 proteins

Boaventura

Buer

Hamaekers

et al. 2020

Pest Management Science

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Background Spodoptera frugiperda, fall armyworm (FAW) is the major pest of maize in Brazil and has readily acquired field resistance to a broad range of synthetic insecticides and to Bacillus thuringiensis (Bt) insecticidal proteins expressed in important crops. This study aims to understand patterns of cross‐resistance in FAW by investigating the toxicological profile of a Bt‐resistant Brazilian strain (Sf_Des) in comparison to a Bt‐susceptible strain (Sf_Bra). Results Laboratory bioassays with 15 active substances of nine mode of action classes revealed that Sf_Des has a medium level of resistance to deltamethrin and chlorpyrifos. Very high cross‐resistance was observed among Cry1 toxins, but high susceptibility against Vip3A. Strain Sf_Des exhibited – depending on the substrate – up to 19‐fold increased cytochrome P450 activity in comparison to Sf_Bra. RNA‐Seq data support a major role of P450 enzymes in the detoxification of insecticides because we detected 85 P450 transcripts upregulated in Sf_Des. Quantitative reverse transcription polymerase chain reaction (RT‐qPCR) analysis confirmed that CYP9A‐like and CYP6B39 are significantly upregulated (>200‐fold) in Sf_Des in comparison to Sf_Bra strain. No target‐site mutation linked to pyrethroid resistance was detected, but mutations in the AChE linked to organophosphate resistance were observed in Sf_Des. A Gene Ontology (GO) analysis of differentially expressed genes (DEG) categorized most of them into the biological process category, involved in oxidation–reduction and metabolic processes. Conclusion Our results indicate that multiple/cross‐resistance mechanisms may have developed in the Sf_Des strain to conventional insecticides and Bt insecticidal proteins. The systematic toxicological analysis presented will help to guide recommendations for an efficient resistance management. © 2020 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Toxicological and molecular profiling of insecticide resistance in a Brazilian strain of fall armyworm resistant to Bt Cry1 proteins

Boaventura

Buer

Hamaekers

et al. 2020

Pest Management Science

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“…Similarly, low intraspecific variation (from 2.3-to 4.8-fold) was observed in baseline susceptibility to diamide, emamectin benzoate, indoxacarb, and the Bacillus thuringiensis (Bt) toxins Cry2Ab and Vip3Aa across H. armigera populations from Australia, Pakistan, India, and Brazil (Ahmad et al, 2003;Kranthi et al, 2009;Bird, 2015;Leite et al, 2018;Pereira et al, 2020). More recently, the response of S. frugiperda populations from Brazil, Mexico, India, and China to organophosphates, pyrethroids, sodium channel block-ers, spinosad, emamectin benzoate, chlorfenapyr, the Bt toxin Cry1F and the S. frugiperda multiple nucleopolyhedrovirus has been reported, with among-population variation in toxicity of individual insecticides ranging from 1.1-to 12-fold within geographical locations (Bentivenha et al, 2019;Kanno et al, 2020;Rivero-Borja et al, 2020;Kaiser et al, 2021;Zhang et al, 2021;Kumar & Mohan, 2022).…”

Section: Discussionmentioning

confidence: 99%

Baseline susceptibility of Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda to the meta‐diamide insecticide broflanilide

et al. 2022

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Broflanilide is a novel meta-diamide insecticide that acts as a γ -aminobutyric acid-gated chloride channel allosteric modulator. With its unique mode of action, broflanilide has no known cross-resistance with existing insecticides and is expected to be an effective tool for the management of insecticide resistance. Establishing the baseline susceptibility to this insecticide is an essential step for developing and implementing effective resistance management strategies. Here we evaluated the baseline susceptibility to broflanilide for 3 cosmopolitan lepidopteran pest species, Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda. Broflanilide exhibited high activity against populations sampled in the major distribution range of these pests in China, with median lethal concentrations (LC 50 ) ranging between 0.209 and 0.684, 0.076 and 0.336, and 0.075 and 0.219 mg/L for H. armigera, P. xylostella, and S. frugiperda, respectively. Amongpopulation variability in susceptibility to broflanilide was moderate for H. armigera (3.3fold), P. xylostella (4.4-fold), and S. frugiperda (2.9-fold). The recommended diagnostic concentrations for H. armigera, P. xylostella, and S. frugiperda were 8, 4, and 2 mg/L, respectively. Little or no cross-resistance to broflanilide was detected in 3 diamide-resistant strains of P. xylostella and 1 spinosyns-resistant strain of S. frugiperda. Our results provide critical information for the development of effective resistance management programs to sustain efficacy of broflanilide against these key lepidopteran pests.

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“…For example, Sheppard and Joyce 27 hypothesize that the NCR they observed is due to the increased enzyme activity of the resistant strain more completely bioactivating chlorfenapyr to its insecticidal form. In another recent analysis not involving NCR, a reduced risk of chlorfenapyr resistance development in Spodoptera frugiperda in Brazil was proposed to be due its obligatory bioactivation 43 …”

Section: Examples Of Pro‐insecticides With Negative Cross‐resistancementioning

confidence: 99%

The potential of pro‐insecticides for resistance management

David

2021

Pest Management Science

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Pro‐insecticides have been a significant part of the insecticide market for decades. Bioactivation of such compounds is generally an enzyme‐controlled process, in which the target insect metabolizes the pro‐form into an active compound. This approach has several potential advantages, including improved bio‐kinetic properties and safety profiles of the pro‐insecticide relative to the active form. A less common advantage of pro‐insecticides is increased activity on metabolically resistant strains. Specific cases in which a pro‐insecticide demonstrates negative cross‐resistance (NCR) on a metabolically resistant strain due to increased bioactivation of the pro‐insecticide have been noted sporadically over the past 50+ years but have not been reviewed before. The purpose of this mini‐review is to catalog the cases in which a pro‐insecticide demonstrated improved activity on an insect strain resistant to a second insecticide via a metabolic mechanism. Cases are relatively rare, but where it does occur the mechanism of NCR is generally recognized as being due to the increased metabolic activity of the resistant strain. These observations can provide learnings with potential application for resistance management if the correct pro‐insecticide is selected for a resistant strain which is better able to bioactivate it. A better understanding of the bioactivation of pro‐insecticides by resistant insects could also aid in insecticide discovery, potentially leading to improved pro‐insecticide design. © 2021 Society of Chemical Industry

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Low risk of resistance evolution of Spodoptera frugiperda to chlorfenapyr in Brazil

Cited by 15 publications

References 59 publications

Toxicological and molecular profiling of insecticide resistance in a Brazilian strain of fall armyworm resistant to Bt Cry1 proteins

Toxicological and molecular profiling of insecticide resistance in a Brazilian strain of fall armyworm resistant to Bt Cry1 proteins

Baseline susceptibility of Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda to the meta‐diamide insecticide broflanilide

The potential of pro‐insecticides for resistance management

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