Low-serum culture system improves the adipogenic ability of visceral adipose tissue-derived stromal cells

Nagasaki, Hiroshi; Shang, Qing Long; Suzuki, Takeshi; Hashimoto, Hiroyuki; Yoshimura, Tomoko; Kondo, Takafumi; Ozaki, Tomoya; Maruyama, Shogo; Jomori, Takahito; Oiso, Yutaka; Hamada, Yoji

doi:10.1042/cbi20100406

Cited by 14 publications

(10 citation statements)

References 25 publications

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“…Hauner's group showed that serum is not required for survival of human preadipocytes during adipogenesis and exposure to serum actually inhibits differentiation (9,14,25), and we confirm these results herein. When our modified 7d‐induction protocol along with well‐differentiating preadipocytes were used, up to 3% FBS did not significantly affect adipogenesis.…”

Section: Discussionsupporting

confidence: 89%

A Modified Protocol to Maximize Differentiation of Human Preadipocytes and Improve Metabolic Phenotypes

Lee

Fried

2012

Obesity

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Adipose stromal cells proliferate and differentiate into adipocytes, providing a valuable model system for studies of adipocyte biology. We compared differentiation protocols for human preadipocytes and report on their metabolic phenotypes. By simply prolonging the adipogenic induction period from the first 3 days to 7 days, the proportion of cells (passage 5–6) acquiring adipocyte morphology increased from 30–70% to over 80% in human subcutaneous preadipocytes. These morphological changes were accompanied by increases in the adipogenic marker expression and improved adipocyte metabolic phenotypes: enhanced responses to beta-adrenergically-stimulated lipolysis and to insulin-stimulated glucose metabolism into triglyceride. Confirming previous studies, FBS dose-dependently inhibited adipogenesis. However, in subcutaneous preadipocytes that differentiate well (donor-dependant high capacity and subcultured fewer than 2 times), the use of 7d-induction protocols in both 3% FBS and serum-free conditions allowed >80% differentiation. Responsiveness to β-adrenergically stimulated lipolysis was lower in 3% FBS. Rates of insulin-stimulated glucose uptake were higher in adipocytes differentiated with 3% FBS, while the sensitivity to insulin was almost identical between the two groups. In summary, extending the length of the induction period in adipogenic cocktail improves the degree of differentiation and responses to key metabolic hormones. This protocol permits functional analysis of metabolic phenotypes in valuable primary human adipocyte cultures through multiple passages.

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Section: Discussionsupporting

confidence: 89%

A Modified Protocol to Maximize Differentiation of Human Preadipocytes and Improve Metabolic Phenotypes

Lee

Fried

2012

Obesity

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“…ADSCs are also a convenient source because of their high proliferation and easy maintenance. 1 × 10 8 ADSCs can be expanded to 10 g of rat epididymal fat pads or human omental fat pads over five passages [42]. Therefore, in the clinical setting, ADSCs could be considered more profitable than BM-MSCs [17].…”

Section: Resultsmentioning

confidence: 99%

Mesenchymal Stem Cells Transplantation following Partial Hepatectomy: A New Concept to Promote Liver Regeneration—Systematic Review of the Literature Focused on Experimental Studies in Rodent Models

Papanikolaou

Katselis

Αποστόλου

et al. 2017

Stem Cells International

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Mesenchymal stem cells (MSCs) are an attractive source for regenerative medicine because they are easily accessible through minimally invasive methods and have the potential to enhance liver regeneration (LG) and improve liver function, following partial hepatectomy (PH) and acute or chronic liver injury. A systematic review of the literature was conducted for articles published up to September 1st, 2016, using the MEDLINE database. The keywords that were used in various combinations were as follows: “Mesenchymal stem cells”, “transplantation”, “stem cells”, “adipose tissue derived stem cells”, “bone marrow-derived stem cells”, “partial hepatectomy”, “acute liver failure”, “chronic liver failure”, “liver fibrosis”, “liver cirrhosis”, “rats”, “mice”, and “liver regeneration”. All introduced keywords were searched for separately in MeSH Database to control relevance and terminological accuracy and validity. A total of 41 articles were identified for potential inclusion and reviewed in detail. After a strict selection process, a total of 28 articles were excluded, leaving 13 articles to form the basis of this systematic review. MSCs transplantation promoted LG and improved liver function. Furthermore, MSCs had the ability to differentiate in hepatocyte-like cells, increase survival, and protect hepatocytes by paracrine mechanisms. MSCs transplantation may provide beneficial effects in the process of LG after PH and acute or chronic liver injury. They may represent a new therapeutic option to treat posthepatectomy acute liver failure.

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“…On the other hand, the outcomes of longer experiments examining the effects of leptin on collagen synthesis and mineralization may be altered by the choice of low serum conditions. Low serum (2%) conditions have been shown to be beneficial for adipogenic differentiation [15] and are detrimental for osteoblast differentiation in general. Ideally, an experiment should examine osteoblast proliferation, differentiation and apoptosis with varying concentrations of leptin, and low and high serum conditions.…”

Section: Contradictory Results From In Vitro Studiesmentioning

confidence: 99%

Understanding leptin-dependent regulation of skeletal homeostasis

Motyl

Rosen

2012

Biochimie

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Despite growing evidence for adipose tissue regulation of bone mass, the role of the adipokine leptin in bone remodeling remains controversial. The majority of in vitro studies suggest leptin enhances osteoblastic proliferation and differentiation while inhibiting adipogenic differentiation from marrow stromal cells. Alternatively, some evidence demonstrates either no effect or a pro-apoptotic action of leptin on stromal cells. Similarly, in vivo work has demonstrated both positive and negative effects of leptin on bone mass. Most of the literature supports the idea that leptin suppresses bone mass by acting in the brainstem to reduce serotonin-dependent sympathetic signaling from the ventromedial hypothalamus to bone. However, other studies have found partly or entirely contrasting actions of leptin. Recently one study found a significant effect of surgery alone with intracerebroventricular administration of leptin, a technique crucial for understanding centrally-mediated leptin regulation of bone. Thus, two mainstream hypotheses for the role of leptin on bone emerge: 1) direct regulation through increased osteoblast proliferation and differentiation and 2) indirect suppression of bone formation through a hypothalamic relay. At the present time, it remains unclear whether these effects are relevant in only extreme circumstances (i.e. models with complete deficiency) or play an important homeostatic role in the regulation of peak bone acquisition and skeletal remodeling. Ultimately, determining the actions of leptin on the skeleton will be critical for understanding how the obesity epidemic may be impacting the prevalence of osteoporosis.

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Low-serum culture system improves the adipogenic ability of visceral adipose tissue-derived stromal cells

Cited by 14 publications

References 25 publications

A Modified Protocol to Maximize Differentiation of Human Preadipocytes and Improve Metabolic Phenotypes

A Modified Protocol to Maximize Differentiation of Human Preadipocytes and Improve Metabolic Phenotypes

Mesenchymal Stem Cells Transplantation following Partial Hepatectomy: A New Concept to Promote Liver Regeneration—Systematic Review of the Literature Focused on Experimental Studies in Rodent Models

Understanding leptin-dependent regulation of skeletal homeostasis

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