Low serum thyroxine due to anti-thyroxine antibody.

Ikekubo, Katsuji; Konishi, Junji; Nakajima, Kazue; Endo, Keigo; Mori, Toru; Torizuka, K

doi:10.1507/endocrj1954.25.275

Cited by 6 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The incidence of anti-T3 or anti-T4 antibodies with varied specificity and dissociation constants in a euthyroid healthy population is about 1.8% (5). The presence of these antibodies interferes with the estimation of total and free hormones by single step immuno assay (3,4,5,6) and may result in very low (undetectable) values (in competitive RIA/EIA that uses precipitation for the separation of bound and free ligand) or spuriously high values (in immunoassays using antibody coated tubes) of either or both T3 and T4 (7,8). The antibodies in…”

Section: Introductionmentioning

confidence: 99%

Interference of anti-T4 autoantibodies in the single step free T4 radioimmunoassays

Rothman

Chawla

1998

Indian J Clin Biochem

View full text Add to dashboard Cite

The thyroid hormone profile of a hypothyroid patient, with moderate sized diffuse goiter and history of exogenous thyroid hormone therapy, was found to be highly ambiguous. The total as well as free T4 levels were consistently high (out of range) with suppressed T3 (total and free) and elevated thyrotropin levels. We incubated the serum with the radio-iodine labelled T4 and observed that the patient's serum bound about 50.5% of the total radioactivity resulting in 85.3% interference in the radioimmunoassay. On resolving the serum proteins by cellulose acetate electrophoresis, the radioactivity was detected in the gamma globulin bend. The protein profile showed mildly raised levels of IgG. Therefore it was established that the patient's serum carried T4 reactive antibodies (IgG) which were interfering in the immunoassays. Elevated anti-thyroid microsomal antibodies were also present in the patient's serum. The anti-T4 antibodies were highly specific for T4 and did not show any cross reactivity with the T3 or its analogues used in the estimation of free T3. We stripped the serum by precipitating the immunoglobulins and were successful in estimating the levels of free T4 in the stripped serum by single step radioimmunoassy.

show abstract

Section: Introductionmentioning

confidence: 99%

Interference of anti-T4 autoantibodies in the single step free T4 radioimmunoassays

Rothman

Chawla

1998

Indian J Clin Biochem

View full text Add to dashboard Cite

show abstract

“…The presence of these antibodies in serum does not onty complicate the clinical manifestations of the disease but also interferes with the estimation of total and free hormones by immuno assay (3,4,5). Their presence may be indicated when very low (undetectable) values of either or both T3 and T4 are observed in a competitive RINEIA that uses precipitation for the separation of" bound and free ligand (6). However, in immunoasays using antibody coated tubes extremely higddh values are…”

mentioning

confidence: 99%

Interference of anti-T3 autoantibodies in the measurement of total and free T3 in serum

Rothman

Chawla

1997

Indian J Clin Biochem

View full text Add to dashboard Cite

A case of anti-tdlodothyronine sutoantibodies is presented in this report. The thyroid hormone profile of a hypertensive patient, with multinodular goiter and history of exogenous thyroid hormone therapy, was found to be highly ambiguous. The total as well as free T3 levels were consistently high (out of range) whereas the T4 (total and flee) values were always within normal limits. The thyrotropin was found to be partially suppressed. Very low T3-Uptake indicated some kind of interference in the immunoassays. We incubated the sera with the radio-iodine labelled T3 and observed that the patient's serum bound about ten times more radioactivity than a control run in parallel. On further resolving the serum proteins on cellulose acetate electrophoresis, the radioactivity was detected in the y-globulin band. Therefore it was established that the patient's serum carried the antibodies reactive with T3 which were interfering in the immunoassays. Elevated anti-thyroid microsomal antibodies were also present in the patient's serum. The anti-T3 antibodies were highly specific for T3 and did not show any cross reactivity with the T4 or its analogues used in the estimation of free T4.

show abstract

Klinische Bedeutung und Nachweismethoden von Autoantikörpern gegen Schilddrüsenhormone

Teuber

Helmke

Mäser

et al. 1980

Verhandlungen Der Deutschen Gesellschaft Für Innere Medizin

View full text Add to dashboard Cite

Low serum thyroxine due to anti-thyroxine antibody.

Cited by 6 publications

References 3 publications

Interference of anti-T4 autoantibodies in the single step free T4 radioimmunoassays

Interference of anti-T4 autoantibodies in the single step free T4 radioimmunoassays

Interference of anti-T3 autoantibodies in the measurement of total and free T3 in serum

Klinische Bedeutung und Nachweismethoden von Autoantikörpern gegen Schilddrüsenhormone

Contact Info

Product

Resources

About